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AGING 2016, Vol. 8, No. 12
Research Paper
Proteins that accumulate with age in human skeletal‐muscle aggregates contribute to declines in muscle mass and function in Caenorhabditis elegans
Srinivas Ayyadevara1,2, Meenakshisundaram Balasubramaniam2,3, Pooja Suri2, Samuel G. Mackintosh4, Alan J. Tackett4, Dennis H. Sullivan1,2,5, Robert J. Shmookler Reis1,2,3,4,5, Richard A. Dennis1,2,5 1
Central Arkansas Veterans Healthcare System, Little Rock, AR 72205, USA Reynolds Institute on Aging, Dept. of Geriatrics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA 3 BioInformatics Program, University of Arkansas at Little Rock and University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA 4 Department of Biochemistry & Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA 5 Geriatric Research, Education and Clinical Center, Central Arkansas Veterans Healthcare System, Little Rock, AR 72205, USA 2
Correspondence to: Srinivas Ayyadevara, Robert J. Shmookler Reis; email:
[email protected];
[email protected] Keywords: protein aggregation, skeletal muscle, aging, sarcopenia, proteostasis Received: September 20, 2016 Accepted: December 3, 2016 Published: December 15, 2016
ABSTRACT Protein aggregation increases with age in normal tissues, and with pathology and age in Alzheimer’s hippocampus and mouse cardiac muscle. We now ask whether human skeletal muscle accumulates aggregates with age. Detergent‐insoluble protein aggregates were isolated from vastus lateralis biopsies from 5 young (23–27 years of age) and 5 older (64–80 years) adults. Aggregates, quantified after gel electrophoresis, contain 2.1‐fold more protein (P
