Quality Assessment of Ojeok-San, a Traditional Herbal Formula, Using ...

Hindawi Publishing Corporation Journal of Analytical Methods in Chemistry Volume 2015, Article ID 607252, 11 pages http://dx.doi.org/10.1155/2015/607252

Research Article Quality Assessment of Ojeok-San, a Traditional Herbal Formula, Using High-Performance Liquid Chromatography Combined with Chemometric Analysis Jung-Hoon Kim,1,2 Chang-Seob Seo,1 Seong-Sil Kim,1 and Hyeun-Kyoo Shin1 1

Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea Division of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 626-870, Republic of Korea

2

Correspondence should be addressed to Hyeun-Kyoo Shin; hkshin@kiom.re.kr Received 22 October 2014; Revised 16 March 2015; Accepted 17 March 2015 Academic Editor: Shixin Deng Copyright © 2015 Jung-Hoon Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Ojeok-san (OJS) is a traditional herbal formula consisting of 17 herbal medicines that has been used to treat various disorders. In this study, quantitative analytical methods were developed using high-performance liquid chromatography equipped with a photodiode array detector to determine 19 marker compounds in OJS preparations, which was then combined with chemometric analysis. The method developed was validated in terms of its precision and accuracy. The intra- and interday precision of the marker compounds were