Structural, dynamic and mechanical properties of POPC at low ...
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Structural, dynamic and mechanical properties of POPC at low ...
Tell us about your experience on SpringerLink and you could win a MacBook Pro. Take survey. Download PDF · European Biophysics Journal. February 2003 ...
Eur Biophys J (2003) 31: 575–585 DOI 10.1007/s00249-002-0253-z
AR TICLE
Michael Rappolt Æ Mo´nica Ferna´ndez Vidal Manfred Kriechbaum Æ Milos Steinhart Heinz Amenitsch Æ Sigrid Bernstorff Æ Peter Laggner
Structural, dynamic and mechanical properties of POPC at low cholesterol concentration studied in pressure/temperature space Received: 4 April 2002 / Accepted: 2 August 2002 / Published online: 25 October 2002 EBSA 2002
Abstract We have studied the structural, dynamic and mechanical properties of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC)/cholesterol binary mixtures by small-angle X-ray scattering. Our investigations were concentrated on the biologically most relevant pressure-temperature-cholesterol regime, i.e. the liquid crystalline phase and its phase boundary to the lamellar gel phase within a cholesterol concentration up to 25 mol%. From the dependence of the transition pressure we derived a value of 19 kJ/mol for the transition enthalpy DHm of POPC in excess water. With increasing cholesterol concentration, DHm drops to about 7 kJ/mol at 20 mol% cholesterol. Time-resolved pressure-scan (p-scan) and temperature-jump (T-jump) experiments reveal that at low cholesterol content (
