Suppl Fig 5 uninfected myeloid exh_v1 - PLOS

A. TIM-3 expression by pulmonary myeloid cells from uninfected mice

100K

Live 68.7

0 0

50K

100K

150K

50K

10

4

3

0

0

200K 250K

0

50K

FSC-A :: FSC-A

100K

150K

0

200K 250K

50K

100K

150K

200K 250K

10

10

10 0

-10

Auto+Ly6Chi 3.29

4

3

2

Auto+Ly6Clo 7.37

2 3

0

SSC-A :: SSC-A

SSC-A :: SSC-A

CD45+

Auto87.4

5

10 10 Comp-B1-A :: Auto-A

4

10

250K

250K

200K

200K

150K 100K 50K

5

0

50K

100K

150K

Single Cells 95.6

150K 100K

Live 78.4

0

50K

Ly6CLoAuto+

10

10 0

-10

4

3

10

CD11c+CD11blo 89.7

10

2

10 0

2 3

4

-10

5

4

CD11cloCD11blo

3

2 2

10

0

50K

10

10 0

-10 103

0

104

10

CD11c+CD11blo 90.8

10

2

10 0

2 0

10

10

4

10

CD11b

10

10

4

10

3

10

CD68+Ly6Glo 99.8

0

0

10

3

10

4

Alv. Macs

10

5

CD68+Ly6G28.1

10

4

10

3

10

DC 62.2

0

5

0

3

10

5

4

10

3

10

4

5

Mac 82.1

10

4

3

CD68-Ly6G11.1

0

0

10

0

10

3

10

4

10

5

0

PMN

CD68+Ly6GNeg

10

3

10

4

10

5

57%

0

10

3

10

4

10

10

10

4

10

3

10

CD68+Ly6Glo 98.3

0

5

0

10

3

10

4

10

3

10

4

10

0

MFI 152

10

3

10

250

68%

4

10

5

0

10

3

5

200K 250K

0

50K

100K

150K

200K 250K

-10

Auto+Ly6Chi 4.02 Auto+Ly6Clo 7.83

2 2

3 4 10 10 Comp-B1-A :: Auto-A

0

SSC-A :: SSC-A

4

3

2

CD11c-CD11bCD11cloCD11bhi 44.3 5.50

2

10

3

10

4

10

5

4

10

3

10

DC 57.3

5

0

10

4

10

5

10

MFI 411

25

95%

20

10

50

5.0

0

0

0

10

3

10

4

4

4

10

3

10

4

10

5

0

10

3

10

4

5

3

0

10

3

10

4

10

CD68-Ly6G21.1

5

0

10

3

10

4

10

5

PMN

Macs

MFI 101 17%

80 60

MFI 319 44%

300

200

100 20

10

0

10

3

10

4

10

0

5

0

DC

10

3

10

4

10

5

0 0

10

3

10

4

10

5

CD68NegLy6GNeg

MFI 44

3%

MFI 27 12%

150

100

50

0 0

TIM-3

Mac 62.7

4

40

150

10

5

0

50

3

10

5

47%

100

10

10

0

15

100

MFI 54

26%

10

CD11bintCD11cint 10

pMN 95.2

Ly6G

CD68NegLy6GNeg

8%

3

5

CD68+Ly6GNeg

MFI 1394

200

10

0

10

CD11bHiCD11clo

CD68+Ly6G28.0

Alv. Macs

MFI 721

1%

DC

0

10 0

3

CD11c+CD11bCD11cloCD11blo 4.49 23.2

5

0

10

150

0

150K

CD11bNegCD11c+

5

Macs

MFI 0

MFI 656

100%

10

PMN

Ly6G

MFI 3723

-10

5

CD11bLoCD11c+

CD68

CD68

10

5

10

CD11b

CD11bLoCD11c+ CD11bNegCD11c+ CD11bHiCD11clo CD11bintCD11cint 10

CD45+ 78.9

Auto83.1

4

Auto

4

105

3

10

5

Neg

10

3

100K

+

5

3

4

SSC-A :: SSC-A

Ly6C Auto 10

5

CD11c

CD11c

10

10

10

10

0

200K 250K

Lo

AutoNeg

5

10

5

0

FSC-A :: FSC-A

10

10

Comp-B2-A :: Ly6C-A

50K

10

10

Comp-V2-A :: CD45-A

100K

150K

CD45+

5

SSC-H :: SSC-H

150K

Comp-V2-A :: CD45-A

200K SSC-H :: SSC-H

SSC-A :: SSC-A

200K

10

Single Cells 92.6

SSC-A :: SSC-A

250K

Comp-B2-A :: Ly6C-A

250K

B. TIM-3 expression by pulmonary myeloid cells from Mtb infected mice

10

3

TIM-3

10

4

10

5

0 0

10

3

10

4

10

5

10

5