Supplementary Material OVEREXPRESSION OF BdMATE GENE IMPROVES ALUMINUM TOLERANCE IN Setaria viridis Ana Paula Ribeiro1,2, Wagner Rodrigo de Souza1, Polyana Kelly Martins1, Felipe Vinecky1, Karoline Estefani Duarte1, Marcos Fernando Basso1, Bárbara Andrade Dias Brito da Cunha1, Raquel Bombarda Campanha1, Patrícia Abrão de Oliveira1, Danilo da Cruz Centeno3, Geraldo Magela de Almeida Cançado4, Jurandir Vieira de Magalhães5, Carlos Antônio Ferreira de Sousa1, Alan Carvalho Andrade2,6, Adilson Kenji Kobayashi1 and Hugo Bruno Correa Molinari1* * Correspondence: Corresponding Author:
[email protected] BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890) BdMATE(Bradig69770.1) SbMATE(Sb03g043890)
MEDG-A----------PTTMTQEKRLA----VVVVAPE-------DPAAAPVLLANGAAA MEEHRSPAHAKPEAEQPPQQQVPAAMAVAVAVDVAAPAALQNSTAAPAENGDVAAAGAAE **: : * :* * *.** ** : * *** EEHKAGEDPPAPPTALLSGWPRRTGLYLFVMNVRSVFKLDELGSEVLRIAVPASLALAAD NGTAASAANGDGGGSELLGGPRWTGLHLFVMNIRSVFKLDELGAEVLGIAVPASLALTAD : *. : * * ** ***:*****:**********:*** *********:** PLASLVDTAFIGRLGSVEIAAVGVSIAIFNQVSKVCIYPLVSVTTSFVAEEDAIISKYLE PLASLIDTAFIGRLGSVEIAAVGVAIAVFNQVMKVCIYPLVSVTTSFVAEEDAVLSKGGA *****:******************:**:**** ********************::** ENSS-----QDLEKAPVDS-----------EASNVPVSG---AECVNSCIPTECT--DLS KVIDNGEEEEELEAGQVGPEKHTAAAGADPEKQQQPADEEAAKNGGEGCAPAVVAGRSSG : . ::** . *. * .: *.. : :.* *: : . . NQGCKRKYIPSVTSALIVGSFLGLVQAVFLIFSAKVVLGIMGVKHDSPMLEPAVRYLKIR KKSGNRRFVPSVTSALIVGALLGLFQTVFLVAAGKPLLRLMGVKPGSPMVMPALRYLTLR ::. :*:::**********::***.*:***: :.* :* :**** .***: **:***.:* SLGAPAVLLSLAMQGVFRGFKDTKTPLYATVVGDAANIILDPILMFVCHMGVTGAAVAHV ALGAPAVLLSLAMQGVFRGFKDAKTPLYAIVAGDAANIVLDPILIFGCRLGVIGAAIAHV :*********************:****** *.******:*****:* *::** ***:*** VSQYLITMILICRLVQQVDVIPPSLKSLKFGRFLGCGFLLLARVVAVTFCVTLASSLAAR LSQYLITLIMLSKLVRKVDVVPPSLKCLKFRRFLGCGFLLLARVVAVTFCVTLAASLAAR :******:*::.:**::***:*****.*** ***********************:***** DGATIMAAFQICCQLWLATSLLADGLAVAGQAVLASAFAKNDNNKVVAATSRVLQLSIVL HGPTAMAAFQICTQVWLATSLLADGLAVAGQAMIASAFAKEDRYKVAATAARVLQLGVVL .* * ******* *:*****************::******:*. **.*:::*****.:** GMGLTVVLGLFMKFGAGVFTKDAAVIDVIHRGIPFVAGTQTINALAFVFDGINFGASDYT GAALTALLGLGLQFGAGVFTSDAAVIKTIRKGVPFVAGTQTLNTLAFVFDGINFGASDYA * .**.:*** ::*******.*****..*::*:********:*:***************: YSAYSMVGVAAISIPCLVYLSAHNGFIGIWVALTIYMSLRTIASTWRMGAARGPWVFLRK FSAYSMIGVAAVSIPSLIFLSSHGGFVGIWVALTIYMGVRALASTWRMAAAQGPWKFLRQ :*****:****:***.*::**:*.**:**********.:*::******.**:*** ***: GW --
Supplementary Figure S5. Alignment of the deduced amino acid sequences. Clustal Omega (Sievers and Higgins, 2014) alignment of the deduced amino acid sequences of Brachypodium distachyon (BdMATE) and Sorghum bicolor (SbMATE) sequences. Symbols under the alignments indicate identical (*), strongly conserved (:) or weakly conserved (.) residues according to the BLOSUM62 matrix.
