Figure S1 Clinical chemistry analysis of mice injected with NAG-MLP plus chol-siRNA. ... ALKP, alkaline phosphatase; BUN, blood urea nitrogen; mean + SD.
Supplementary Materials Figure S1 Clinical chemistry analysis of mice injected with NAG-MLP plus chol-siRNA. ICR mice were injected intravenously with 2 mg/kg chol-siF7 or chol-siLuc and 1 – 16 mg/kg NAGMLP (n = 4). Serum samples were collected 48 hr later and evaluated for markers of liver or kidney damage; IG, isotonic glucose; ALT, alanine transaminase; AST, aspartate transaminase; ALKP, alkaline phosphatase; BUN, blood urea nitrogen; mean + SD.
1
Figure S2 Controls for NAG-MLP and chol-siRNA co-injection in the pHBV mouse model. (a) NOD SCID mice were given a hydrodynamic tail vein injection with 10 µg MC-HBV1.3. Three weeks later, mice were given one 200 µL IV injection of isotonic glucose alone (n = 8) or 6 mg/kg NAG-MLP alone (n = 7); an injection of 6 mg/kg chol-siHBV-74 plus chol-siHBV-77 (3 mg/kg each) without NAG-MLP (n = 4); a co-injection of 6 mg/kg NAG-MLP with 6 mg/kg chol-siF7p (primate F7) (n = 4); or a co-injection of the indicated doses of NAG-MLP with equal doses of chol-siHBV-74, chol-siHBV-77, or chol-siHBV-74 plus chol-siHBV-77 (n = 4-11). HBsAg was measured in serum at the indicated time points before and after injection, mean ± SD. (b) ALT and AST were measured in serum from the mice shown in (a) that were injected with isotonic glucose or co-injected with 3 mg/kg NAG-MLP and 3 mg/kg chol-siHBVs (cholsiHBV-74 or chol-siHBV-77), or 6 mg/kg NAG-MLP and 6 mg/kg chol-siHBVs (chol-siHBV74, chol-siHBV-77, or chol-siHBV-74+77). Measurements were taken at the indicated times relative to IV injection of samples and combined for the indicated treatment groups. ALT and AST levels are also shown for a control group of NOD SCID mice that had been injected with 10 µg MC-HBV1.3 but with no IV injection of sample (no treatment) for comparison.
2
3
Figure S3 Clinical chemistry in transgenic HBV1.3.32 mice. HBV1.3.32 mice were injected on day 1 and day 8 with 6 mg/kg NAG-MLP and 3 mg/kg of the indicated chol-siRNA or a combination of 3 mg/kg chol-siHBV-74 plus 3 mg/kg chol-siHBV-77 (n = 3 - 4). Serum was collected one day before the first injection and one day after each injection and evaluated for clinical chemistries; ALT, alanine transaminase; AST, aspartate transaminase; GLDH, glutamate dehydrogenase; BUN, blood urea nitrogen; mean + SD.
4
Table S1. Cytokine/chemokine evaluation of NAG-MLP-injected non-human primates. Serum was collected from cynomolgus monkeys 4 days prior to NAG-MLP plus chol-siRNA injection, 6 hr post-injection, and 4 days post-injection, and then evaluated for levels of cytokines and chemokines.
