Supplementary Table S1. 16S rRNA gene sequence ...

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rpo-77 rpo-115 rpo-68 rpo-74 rpo-04 rpo-92 rpo-37b rpo-11 rpo-95 rpo-05 rpo-03 rpo-88 rpo-17 rpo-40 rpo-67 rpo-01. rpoB-Anaeslundii MG1 rpo-60 rpo-102.
Supplementary Table S1. 16S rRNA gene sequence similarity between groups Values are mean percentages calculated using Kimura’s two-parameter correction. Group A. naeslundii A. johnsonii sp. nov. A. viscosus

A. oris sp. nov. Genospecies 2 99.2 98.7 99.2

A. naeslundii Genospecies 1 – 98.9 99.2

A. johnsonii sp. nov. Serotype WVA 963 – 98.5

Supplementary Table S2. Strains used and accession numbers for partial 16S rRNA gene sequences Strain P2G P6N P5K P6K P7K P8K P9K Pn4D Pn5D Pn1GA Pn16E P10N Pn20E P5N [A. viscosus] ATCC 27044T P11N (=CCUG 33920) PK1259 (=CCUG 33932) VPI 12593 (=CCUG 34285) VPI D163E-3 (=CCUG 34286) 461 (=CCUG 34725) TF 11 (=CCUG 35334) R709-03041/97 (=CCUG 37599) CCUG 34287T

Accession number EU667389 EU667390 EU667391 EU667392 EU667393 EU667394 EU667395 EU667396 EU667397 EU667398 EU667399 EU667400 EU667401 EU667402 EU667403 EU667404 EU667405 EU667406 EU667407 EU667408 EU667409 EU667410 EU667411

Species name A. oris A. oris A. oris A. oris A. oris A. oris A. oris A. oris A. oris A. naeslundii A. naeslundii A. oris A. naeslundii A. oris A. oris A. oris A. johnsonii A. oris A. oris A. naeslundii A. naeslundii A. naeslundii A. johnsonii

Source Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg Strömberg ATCC CCUG CCUG CCUG CCUG CCUG CCUG CCUG CCUG

Study no. 95 96 97 98 99 100 101 102 103 104 105 106 108 110 119 111 117 115 116 112 113 114 118

Henssge, U., Do, T., Radford, D. R., Gilbert, S. C., Clark, D. & Beighton, D. (2009). Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963. Int J Syst Evol Microbiol 59, 509–516.

Supplementary Table S3. Details of oral and non-oral isolates and reference and type strains used in this study and GenBank accession numbers of partial sequences Study no.

Isolate

Oral and non-oral clinical isolates 01 A18A-3 02 A19A-1 03 A2A-1 04 A3A-1 05 A6A-1 06 A7A-1 07 E1-20 08 F11C1 09 F12B1 11 F1A1 12 F21A1 13 F23A1 14 F24C1 15 F2A12 16 F4D1 17 F5A10 18 F6E1 19b G126D 20b G127B 21 G128C 23 G140C 24 G48B 25 G51C 26 G53E 27 G54C 28 T14P-1 29b T17-3 30 T20P-1 31 T22P-A1 32 T23P-1 33 T5P-1 34 T6P-2 35 T8-1 36 T9P-1 37b UN4B6c 38 W11-1-1 39c W8-2-3 40 WE10B-1 41 WE1Aa1

Origin

Leathery lesion Leathery lesion Leathery lesion Leathery lesion Leathery lesion Leathery lesion Plaque (caries –) Plaque (caries +) Plaque (caries +) Plaque (caries –) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries +)

atpA

gltA

EU620779 EU620780 EU620781 EU620782 EU620783 EU620784 EU620785 EU620786 EU620787 EU620788 EU620789 EU620790 EU620791 EU620792 EU620793 EU620794 EU620795 EU620796 EU620797 EU620798 EU620799 EU620800 EU620801 EU620802 EU620803 EU620804 EU620805 EU620806 EU620807 EU620808 EU620809 EU620810 EU620811 EU620812 EU620813 EU620814 EU620815 EU620816 EU620817

EU620895 EU620896 EU620897 EU620898 EU620899 EU620900 EU620901 EU620902 EU620903 EU620904 EU620905 EU620906 EU620907 EU620908 EU620909 EU620910 EU620911 EU620912 EU620913 EU620914 EU620915 EU620916 EU620917 EU620918 EU620919 EU620920 EU620921 EU620922 EU620923 EU620924 EU620925 EU620926 EU620927 EU620928 EU620929 EU620930 EU620931 EU620932 EU620933

GenBank accession no. gyrA metG EU621011 EU621012 EU621013 EU621014 EU621015 EU621016 EU621017 EU621018 EU621019 EU621020 EU621021 EU621022 EU621023 EU621024 EU621025 EU621026 EU621027 EU621028 EU621029 EU621030 EU621031 EU621032 EU621033 EU621034 EU621035 EU621036 EU621037 EU621038 EU621039 EU621040 EU621041 EU621042 EU621043 EU621044 EU621045 EU621046 EU621047 EU621048 EU621049

EU621127 EU621128 EU621129 EU621130 EU621131 EU621132 EU621133 EU621134 EU621135 EU621136 EU621137 EU621138 EU621139 EU621140 EU621141 EU621142 EU621143 EU621144 EU621145 EU621146 EU621147 EU621148 EU621149 EU621150 EU621151 EU621152 EU621153 EU621154 EU621155 EU621156 EU621157 EU621158 EU621159 EU621160 EU621161 EU621162 EU621163 EU621164 EU621165

pgi

rpoB

EU603149 EU603150 EU603151 EU603152 EU603153 EU603154 EU603155 EU603156 EU603157 EU603158 EU603159 EU603160 EU603161 EU603162 EU603163 EU603164 EU603165 EU603166 EU603167 EU603168 EU603169 EU603170 EU603171 EU603172 EU603173 EU603174 EU603175 EU603176 EU603177 EU603178 EU603179 EU603180 EU603181 EU603182 EU603183 EU603184 EU603185 EU603186 EU603187

EU621243 EU621244 EU621245 EU621246 EU621247 EU621248 EU621249 EU621250 EU621251 EU621252 EU621253 EU621254 EU621255 EU621256 EU621257 EU621258 EU621259 EU621260 EU621261 EU621262 EU621263 EU621264 EU621265 EU621266 EU621267 EU621268 EU621269 EU621270 EU621271 EU621272 EU621273 EU621274 EU621275 EU621276 EU621277 EU621278 EU621279 EU621280 EU621281

Henssge, U., Do, T., Radford, D. R., Gilbert, S. C., Clark, D. & Beighton, D. (2009). Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963. Int J Syst Evol Microbiol 59, 509–516.

Study no. 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 60 61 62 64 65 66 67 68 69 70 71 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87

Isolate WE3Bc14 WE4Bb1 WE5Ba2 WE6B-3 WE7B1 S24V S29C S33A S38H S41C S43L S44D S47B S49H S53C S55N S56D S62B S64C S65A U136-1 U149-1 MMRCO1-1 MMRCO2-1 MMRCO6-1 M42-1-1 M46-1-1 M47-1-1 F7j1 F28B1 WE8B-23 WE9A10-1 M48-1B-1 M67-1 MB-1 T12-1 T18-1 MMRC12-1 R6630† R8152† R9841† R11372† R13240†

Origin Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Plaque (caries –) Leathery lesion Leathery lesion Soft lesion Soft lesion Soft lesion Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries –) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries +) Plaque (caries –) Plaque (caries –) Soft lesion Knee fluid IUCD Aortic valve IUCD Subphrenic abscess

atpA EU620818 EU620819 EU620820 EU620821 EU620822 EU620823 EU620824 EU620825 EU620826 EU620827 EU620828 EU620829 EU620830 EU620831 EU620832 EU620833 EU620834 EU620835 EU620836 EU620837 EU620838 EU620839 EU620840 EU620841 EU620842 EU620843 EU620844 EU620845 EU620846 EU620847 EU620848 EU620849 EU620850 EU620851 EU620852 EU620853 EU620854 EU620855 EU620856 EU620857 EU620858 EU620859 EU620860

gltA EU620934 EU620935 EU620936 EU620937 EU620938 EU620939 EU620940 EU620941 EU620942 EU620943 EU620944 EU620945 EU620946 EU620947 EU620948 EU620949 EU620950 EU620951 EU620952 EU620953 EU620954 EU620955 EU620956 EU620957 EU620958 EU620959 EU620960 EU620961 EU620962 EU620963 EU620964 EU620965 EU620966 EU620967 EU620968 EU620969 EU620970 EU620971 EU620972 EU620973 EU620974 EU620975 EU620976

GenBank accession no. gyrA metG EU621050 EU621166 EU621051 EU621167 EU621052 EU621168 EU621053 EU621169 EU621054 EU621170 EU621055 EU621171 EU621056 EU621172 EU621057 EU621173 EU621058 EU621174 EU621059 EU621175 EU621060 EU621176 EU621061 EU621177 EU621062 EU621178 EU621063 EU621179 EU621064 EU621180 EU621065 EU621181 EU621066 EU621182 EU621067 EU621183 EU621068 EU621184 EU621069 EU621185 EU621070 EU621186 EU621071 EU621187 EU621072 EU621188 EU621073 EU621189 EU621074 EU621190 EU621075 EU621191 EU621076 EU621192 EU621077 EU621193 EU621078 EU621194 EU621079 EU621195 EU621080 EU621196 EU621081 EU621197 EU621082 EU621198 EU621083 EU621199 EU621084 EU621200 EU621085 EU621201 EU621086 EU621202 EU621087 EU621203 EU621088 EU621204 EU621089 EU621205 EU621090 EU621206 EU621091 EU621207 EU621092 EU621208

pgi EU603188 EU603189 EU603190 EU603191 EU603192 EU603193 EU603194 EU603195 EU603196 EU603197 EU603198 EU603199 EU603200 EU603201 EU603202 EU603203 EU603204 EU603205 EU603206 EU603207 EU603208 EU603209 EU603210 EU603211 EU603212 EU603213 EU603214 EU603215 EU603216 EU603217 EU603218 EU603219 EU603220 EU603221 EU603222 EU603223 EU603224 EU603225 EU603226 EU603227 EU603228 EU603229 EU603230

rpoB EU621282 EU621283 EU621284 EU621285 EU621286 EU621287 EU621288 EU621289 EU621290 EU621291 EU621292 EU621293 EU621294 EU621295 EU621296 EU621297 EU621298 EU621299 EU621300 EU621301 EU621302 EU621303 EU621304 EU621305 EU621306 EU621307 EU621308 EU621309 EU621310 EU621311 EU621312 EU621313 EU621314 EU621315 EU621316 EU621317 EU621318 EU621319 EU621320 EU621321 EU621322 EU621323 EU621324

Henssge, U., Do, T., Radford, D. R., Gilbert, S. C., Clark, D. & Beighton, D. (2009). Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963. Int J Syst Evol Microbiol 59, 509–516.

Study no.

Isolate

88 R14746† 89 R19039† 90 R21091† 91 R23087† 92 R23275† 93 R23277† 94 R24330† Reference and type strains 95 P2G 96 P6N 97 P5K 98 P6K 99 P7K 100 P8K 101 P9K 102 Pn4D 103 Pn5D 104 Pn1GA 105 Pn16E 106 P10N 107 Pn6N 108 Pn20E 109 A. naeslundii ATCC 12104T 110 P5N 111 CCUG 33920 112 CCUG 34725 113 CCUG 35334 114 CCUG 37599 115 CCUG 34285 116 CCUG 34286 117 CCUG 33932 118 A. johnsonii CCUG 34287T 119 A. oris ATCC 27044T 120 A. viscosus NCTC 10951T

Origin Axilla Liver abscess Cerebral abscess Blood culture Blood culture Blood culture IUCD

atpA EU620861 EU620862 EU620863 EU620864 EU620865 EU620866 EU620867

gltA EU620977 EU620978 EU620979 EU620980 EU620981 EU620982 EU620983

GenBank accession no. gyrA metG EU621093 EU621209 EU621094 EU621210 EU621095 EU621211 EU621096 EU621212 EU621097 EU621213 EU621098 EU621214 EU621099 EU621215

pgi EU603231 EU603232 EU603233 EU603234 EU603235 EU603236 EU603237

rpoB EU621325 EU621326 EU621327 EU621328 EU621329 EU621330 EU621331

EU620868 EU620869 EU620870 EU620871 EU620872 EU620873 EU620874 EU620875 EU620876 EU620877 EU620878 EU620879 EU620880 EU620881 EU620882 EU620883 EU620884 EU620888 EU620889 EU620890 EU620886 EU620887 EU620885 EU620891 EU620892 EU620893

EU620984 EU620985 EU620986 EU620987 EU620988 EU620989 EU620990 EU620991 EU620992 EU620993 EU620994 EU620995 EU620996 EU620997 EU620998 EU620999 EU621000 EU621004 EU621005 EU621006 EU621002 EU621003 EU621001 EU621007 EU621008 EU621009

EU621100 EU621101 EU621102 EU621103 EU621104 EU621105 EU621106 EU621107 EU621108 EU621109 EU621110 EU621111 EU621112 EU621113 EU621114 EU621115 EU621116 EU621120 EU621121 EU621122 EU621118 EU621119 EU621117 EU621123 EU621124 EU621125

EU603238 EU603239 EU603240 EU603241 EU603242 EU603243 EU603244 EU603245 EU603246 EU603247 EU603248 EU603249 EU603250 EU603251 EU603252 EU603253 EU603254 EU603258 EU603259 EU603260 EU603256 EU603257 EU603255 EU603261 EU603262 EU603263

EU621332 EU621333 EU621334 EU621335 EU621336 EU621337 EU621338 EU621339 EU621340 EU621341 EU621342 EU621343 EU621344 EU621345 EU621346 EU621347 EU621348 EU621352 EU621353 EU621354 EU621350 EU621351 EU621349 EU621355 EU621356 EU621357

EU621216 EU621217 EU621218 EU621219 EU621220 EU621221 EU621222 EU621223 EU621224 EU621225 EU621226 EU621227 EU621228 EU621229 EU621230 EU621231 EU621232 EU621236 EU621237 EU621238 EU621234 EU621235 EU621233 EU621239 EU621240 EU621241

*Caries +, Isolate from subject with active occlusal carie; caries –, isolate from caries-free subject. †Isolates purchased from Dr Val Hall (Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Cardiff, UK).

Henssge, U., Do, T., Radford, D. R., Gilbert, S. C., Clark, D. & Beighton, D. (2009). Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963. Int J Syst Evol Microbiol 59, 509–516.

(a) rpoB

0.005

88

83 74

rpo-41 rpo-119T rpo-12 rpo-14 rpo-71 rpo-08 rpo-34 rpo-81 rpo-31 rpo-65 rpo-33 rpo-58 rpo-91 rpo-44 rpo-78 rpo-13 rpo-93 rpo-26 rpo-76 rpo-80 rpo-99 rpo-100 rpo-06 rpo-101 rpo-98 rpo-70 rpo-97 rpo-02 rpo-116 rpo-86 rpo-75 rpo-77 rpo-115 rpo-68 rpo-74 rpo-04 rpo-92 rpo-37b rpo-11 rpo-95 rpo-05 rpo-03 rpo-88 rpo-17 rpo-40 rpo-67 rpo-01 rpoB-Anaeslundii MG1 rpo-60 rpo-102 rpo-103 rpo-47 rpo-61 rpo-90 rpo-73 rpo-16 rpo-42 rpo-43 rpo-27 rpo-21 rpo-38 rpo-48 rpo-24 rpo-23 rpo-57 rpo-54 rpo-83 rpo-35 rpo-66 rpo-15 rpo-49 rpo-111 rpo-110 rpo rpo-96 rpo-106 rpo-120 rpo-114 rpo-18 rpo-82 rpo-107 rpo-64 rpo-32 rpo-51 rpo-50 rpo-07 rpo-56 rpo-30 rpo-19b rpo-109T rpo-25 25 rpo-113 rpo-94 rpo-20b rpo-28 rpo-55 rpo-104 rpo-112 rpo-53 rpo-79 rpo-45 rpo-89 rpo-46 rpo-09 rpo-39c rpo-87 rpo-84 rpo-62 rpo-105 rpo-52 rpo-29b rpo-85 rpo-69 rpo-36 rpo-108 rpo-118T rpo-117 99

(b) gyrA

A. oris sp. nov.

92

81

A. naeslundii

A. johnsonii sp. nov.

0.005

gyr-04 gyr-17 gyr-08 gyr-40 gyr-37b gyr-03 gyr-27 gyr-100 gyr-97 gyr-101 gyr-99 gyr-98 gyr-06 gyr-67 gyr-31 gyr-14 gyr-119T gyr-12 gyr-71 gyr-70 gyr-93 gyr-80 gyr-16 gyr-73 gyr-13 gyr-76 gyr-58 gyr-44 gyr-01 gyr-78 gyr-26 gyr-91 gyr-115 gyr-33 gyr-34 gyr-41 gyr-65 gyr-81 gyrA-Anaeslundii MG1 gyr-102 gyr-103 gyr-60 gyr-11 gyr-95 gyr-42 gyr-57 gyr-38 gyr-54 gyr-23 gyr-66 gyr-35 gyr-43 gyr-48 gyr-49 gyr-21 gyr-15 gyr-88 gyr-24 gyr-83 gyr-77 gyr-75 gyr-68 gyr-74 gyr-05 gyr-92 gyr-111 gyr-61 gyr-90 gyr-47 gyr-106 gyr-96 gyr-110 gyr-116 gyr-02 gyr-86 gyr-118T 99 gyr-117 gyr-120 gyr-36 gyr-79 gyr-55 gyr-112 gyr-94 gyr-07 gyr-53 gyr-19b gyr-28 gyr-32 gyr-62 99 gyr-85 gyr-20b gyr-25 gyr-56 gyr-52 gyr-50 gyr-51 gyr-64 gyr-104 gyr-18 gyr-82 gyr-107 gyr-114 gyr-46 gyr-84 gyr-45 gyr-39c gyr-87 -87 gyr-09 gyr-89 gyr-29b gyr-69 gyr-30 gyr-109T gyr-105 gyr-108 gyr-113

A. oris sp. nov.

A. johnsonii sp. nov.

A. naeslundii

Supplementary Fig. S1. Phylogenetic relationships between A. naeslundii genospecies 1 (A. naeslundii sensu stricto), genospecies 2 (A. oris sp. nov.) and serotype WVA 963 (A. johnsonii sp. nov.). Neighbour-joining trees were determined by partial gene sequence analysis of rpoB (a), gyrA (b), pgi (c) and gltA (d). Type and reference strains are identified by study numbers (see Table 1) and indicated as follows: z, A. oris sp. nov. (genosp. 2); „, A. naeslundii (genosp. 1); ‘, A. viscosus; c, A. johnsonii sp. nov. (genosp. WVA 963). Bootstrap values are indicated at corresponding nodes. Bars, 0.005 substitutions per site. Henssge, U., Do, T., Radford, D. R., Gilbert, S. C., Clark, D. & Beighton, D. (2009). Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963. Int J Syst Evol Microbiol 59, 509–516.

(c) pgi

pgi-14 pgi-71 pgi-12 pgi-06 pgi-119T pgi-41 41 pgi-31 pgi-34 pgi-70 pgi-01 pgi-11 pgi-80 pgi-88 pgi-95 pgi-Anaeslundii MG1 pgi-08 pgi-67 pgi-17 pgi-98 pgi-97 pgi-99 pgi-100 pgi-101 pgi-40 pgi-92 pgi-04 pgi-05 pgi-65 pgi-60 pgi-115 pgi-26 pgi-91 pgi-44 pgi-78 pgi-58 pgi-38 pgi-66 pgi-43 pgi-76 pgi-93 pgi-13 pgi-15 pgi-48 pgi-57 pgi-49 pgi-54 pgi-42 pgi-21 pgi pgi-03 pgi-73 pgi-24 pgi-83 pgi-23 pgi-35 pgi-47 pgi-61 pgi-90 pgi-16 pgi-102 pgi-103 pgi-81 pgi-27 pgi-33 pgi-74 pgi-77 pgi-68 pgi-37b pgi-86 pgi-116 pgi-75 pgi-02 pgi-53 pgi-32 pgi-64 pgi-51 pgi-82 pgi-50 70 pgi-104 pgi-46 pgi-87 pgi-09 pgi-89 pgi-45 pgi-84 pgi-20b pgi-25 pgi-28 pgi-36 pgi-52 pgi-19b pgi-79 pgi-07 pgi-18 pgi-39c pgi-114 pgi-107 pgi-56 pgi-55 pgi-108 pgi-29b pgi-94 pgi-62 pgi-109T pgi-85 pgi-69 pgi-112 pgi-105 pgi-30 pgi-113 pgi-110 pgi-106 pgi-96 -96 pgi-111 pgi-120 pgi-118T pgi-117 99

glt-16 glt-83 glt-35 glt-66 glt-49 glt-38 glt-48 glt-43 glt-24 glt-54 glt-21 glt-23 glt-93 glt-13 glt-15 glt-73 glt-76 glt-03 glt-42 glt-57 glt-33 glt-58 glt-65 glt-34 glt-40 glt-41 glt-47 glt-61 glt-90 glt-96 glt-106 glt-110 glt-02

(d) gltA

A. oris sp. nov.

4

44

89

0.005

glt-116 glt-86 glt-77 glt-68 glt-74

A. oris sp. nov.

glt-01 glt-70 glt-80 glt-95 glt-11 glt-27 glt-91 glt-115 glt-31 glt-81 glt-26 glt-88 glt-44 glt-78 glt-71 glt-67 glt-97 glt-100 glt-98 glt-99 glt-101 glt-92

38

glt-102 glt-103 gltA-Anaeslundii MG1 glt-60 glt-05 glt-12 glt-37b glt-08 glt-04 glt-17 glt-75

49

glt-06 glt-14 glt-119T glt-111 glt-120

25

49

A. naeslundii

glt-09 glt-89 glt-84 glt-39c glt-45 glt-46 glt-19b glt-105 glt-108 glt-07 glt-29b glt-69 glt-79 glt-53 glt-52 glt-114 glt-87 glt-18 glt-94 glt-82 glt-107 glt-36 glt-20b glt-28 glt-25 glt-30 glt-55 glt-112 glt-32 glt-50 glt-51 glt-64 glt-104 glt-62 glt-113 glt-109T glt-56 glt-85

A. naeslundii

glt-117

A. johnsonii sp. nov.

99

glt-118T

A. johnsonii sp. nov.

0.005

Supplementary Fig. S1. cont.

Henssge, U., Do, T., Radford, D. R., Gilbert, S. C., Clark, D. & Beighton, D. (2009). Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963. Int J Syst Evol Microbiol 59, 509–516.