Supporting document for identification of Acinetobacter sp CBT01
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Supporting document for identification of Acinetobacter sp CBT01
The isolate is a gram-negative bacteria with short rods (cocobacilli) of 1-2µm in size (figure 1). The morphological and biochemical characteristics of isolate are ...
Supporting document for identification of Acinetobacter sp CBT01 Morphological and Biochemical Characterization: The colony morphology of the isolate was observed to be circular with entire margins and opaque. The isolate is a gram-negative bacteria with short rods (cocobacilli) of 1-2µm in size (figure 1). The morphological and biochemical characteristics of isolate are given in the Table 1 and 2 Table 1: Morphological and physiological characteristics of isolate Tests
Results
Colony morphology Configuration
Circular
Margin
Entire
Elevation
Convex
Surface
Smooth
Pigment
Off-white
Opacity
Opaque
Gram’s reaction
negative
Cell shape
Rods
Size (m)
1-2m
Spore(s)
-
Motility
Non-motile
Physiological characteristics Growth at temperatures 4C
-
10C
+
25C
+
30C
+
37C
+
42C
+
55C
+
Growth at pH pH 4.0
-
pH 5.0
+
pH 6.0
+
pH 7.0
+
pH 8.0
+
pH 9.0
+
pH 10.0
+
pH 11.0
+
Table 2: Biochemical Characteristics of isolate
Substrate, Test
Result
Gram Staining
Negative
Citrate
Positive
Catalase
Positive
Gelatin liquefaction
Negative
Nitrate reduction
Negative
Oxidase
Negative
Acid production from Glucose
Positive
Sucrose
Positive
Mannose
Positive
Lactose
Negative
Indole
Negative
Methyl red
Positive
Voges-Proskauer
Negative
Figure 1 Scanning electron microscope of isolate at magnification of 10x.
