Supporting_Information_p Values

Supplementary Table S1 Listing of exact p -Values and Statistical Tests in each sub-Figure Figure 1A 2 weeks Chow

AST C8-WT C8-C8R3 C8-R3

p values 0,0292 0,009 0,0295

2 weeks MCD

C8-WT C8-C8R3 C8-R3 WT-C8R3 WT-R3

0,0119 0,0015 0,0019 0,05 0,0422

Mann Whitney test Unpaired t test with Welch's correction Unpaired t test with Welch's correction Mann Whitney test Mann Whitney test

8 weeks MCD

C8-WT C8-C8R3 C8-R3 WT-C8R3 WT-R3

0,0079 0,0119 0,0119 0,0062 0,0098

Mann Whitney test Mann Whitney test Mann Whitney test Unpaired t-test Unpaired t test with Welch's correction

0,0004 0,0001 0,0001 0,0001

Mann Whitney test Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

ALT C8-WT C8-C8R3 C8-R3

p values 0,0312 0,0091 0,0071

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

C8-WT C8-C8R3 C8-R3 WT-C8R3 WT-R3

0,0128 0,0001 0,0001 0,0377 0,0422

Unpaired t-test Unpaired t-test Unpaired t-test Unpaired t-test Unpaired t test with Welch's correction

0,0001 0,0002 0,0022 0,0005

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Mann Whitney test Mann Whitney test

p values 0,0149 0,0154 0,0159

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

2w MCD vs 2w Chow WT-WT C8-C8 C8R3-C8R3 R3-R3

2 weeks MCD

8 weeks MCD

2w MCD vs 2w Chow WT-WT C8-C8 C8R3-C8R3 R3-R3

2 weeks Chow

2 weeks MCD

GLDH C8-WT C8-C8R3 C8-R3 C8-WT

0,0039

Mann Whitney test Unpaired t-test Unpaired t-test

Mann Whitney test

8 weeks MCD

C8-C8R3 C8-R3

0,0048 0,0006

Mann Whitney test Mann Whitney test

C8-WT C8-C8R3 C8-R3 WT-C8R3 WT-R3

0,0017 0,0001 0,0002 0,0026 0,0071

Unpaired t-test Unpaired t-test Unpaired t test with Welch's correction Unpaired t-test Unpaired t test with Welch's correction

0,0004 0,0057 0,0001 0,0005

Mann Whitney test Mann Whitney test Unpaired t test with Welch's correction Mann Whitney test

RIP3 C8-WT C8-C8R3 C8-R3 WT-C8R3 WT-R3 WT-WTnc C8-WTnc

p values 0,0115 0,0069 0,0068 0,0008 0,0042 0,0179 0,0179

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t-test Unpaired t test with Welch's correction Mann Whitney test Mann Whitney test

Ki67 C8-C8R3 C8-R3 WT-C8R3 WT-R3 WT-WTnc C8-WTnc

p values 0,0089 0,0016 0,0003 0,0005 0,0026 0,0043

Unpaired t test with Welch's correction Unpaired t-test Unpaired t-test Unpaired t-test Unpaired t-test Unpaired t-test

TG WT-C8R3

p values 0,0365

Unpaired t-test

Fibrotic Area C8-WT C8-C8R3 C8-R3 WT-C8R3 WT-R3 WT-WTnc C8-WTnc

p values 0,0366 0,0057 0,0062 0,0417 0,0483 0,0402 0,0056

Unpaired t-test Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

Col1 α 1 C8-WT C8-C8R3

p values 0,0307 0,0478

Unpaired t test with Welch's correction Unpaired t test with Welch's correction

2w MCD vs 2w Chow WT-WT C8-C8 C8R3-C8R3 R3-R3 Figure 1D

Figure 2B 8 weeks MCD Figure 2D

Figure 3B

Figure 3C

Figure 3D

C8-R3 WT-C8R3 WT-R3 WT-WTnc C8-WTnc

0,045 0,0313 0,0426 0,0373 0,0438

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

CD45 C8-WT C8-C8R3

p values 0,0408 0,0252

Unpaired t test with Welch's correction Unpaired t test with Welch's correction

F4/80 C8-C8R3 C8-R3 WT-C8R3 WT-R3 WT-WTnc C8-WTnc

p values 0,0302 0,0355 0,0001 0,0001 0,0097 0,0097

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t-test Unpaired t-test Mann Whitney test Mann Whitney test

MCP-1 C8-WT C8-C8R3 C8-R3 WT-R3 C8R3-R3 WT-WTnc C8-WTnc

p values 0,0079 0,0119 0,008 0,0173 0,0173 0,0237 0,0001

Unpaired t-test Unpaired t-test Unpaired t-test Unpaired t-test Unpaired t-test Unpaired t test with Welch's correction Unpaired t test with Welch's correction

MCP-1 C8-WT C8-C8R3 C8-R3 WT-C8R3 WT-R3 WT-WTnc C8-WTnc C8R3-WTnc

p values 0,0215 0,0098 0,006 0,0388 0,036 0,0039 0,0045 0,0052

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t-test Unpaired t-test Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

MCP-1 C8-C8R3 C8-R3 WT-C8R3 WT-R3 WT-WTnc C8-WTnc C8R3-WTnc R3-WTnc

p values 0,0295 0,034 0,0226 0,0243 0,0018 0,0121 0,0483 0,0029

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

Figure 4B

Figure 6B

Figure 6C

Figure 6D

Figure 6E

Sup Fig 3B

MCP-1 C8-WT C8-C8R3 C8-R3 WT-R3 WT-WTnc C8-WTnc

p values 0,0465 0,0159 0,0079 0,0003 0,0039 0,0022

Mann Whitney test Mann Whitney test Mann Whitney test Unpaired t-test Unpaired t test with Welch's correction Mann Whitney test

CK-19 C8-WT C8-C8R3 C8-R3 C8-WTnc

p values 0,0259 0,025 0,0084 0,0237

Unpaired t-test Unpaired t-test Unpaired t-test Unpaired t test with Welch's correction

p-cJUN DMSO-SP6

p values 0,0056

Unpaired t test with Welch's correction

AST DMSO-SP6 ALT DMSO-SP6 GLDH DMSO-SP6

p values 0,0135 p values 0,0437 p values 0,0127

Col1 α 1 DMSO-SP6

p values 0,021

CD45 DMSO-SP6 F4/80 DMSO-SP6

p values 0,043 p values 0,0103

Fibrotic Area C8-WT C8-C8R3 C8-R3 WT-C8R3 WT-R3 WT-WTnc C8-WTnc

p values 0,0001 0,0001 0,0001 0,0191 0,0112 0,003 0,0001

Mann Whitney test Mann Whitney test Mann Whitney test Unpaired t test Unpaired t test with Welch's correction Unpaired t test with Welch's correction Mann Whitney test

Col1 α 1 C8-WT C8-C8R3 C8-R3

p values 0,0026 0,0001 0,003

Unpaired t test with Welch's correction Unpaired t-test Unpaired t test with Welch's correction

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

Unpaired t test

Unpaired t test with Welch's correction Unpaired t-test

Sup Fig 4B

Sup Fig 5B

Sup Fig 7A

Sup Fig 7B

Sup Fig 9

WT-WTnc C8-WTnc C8R3-WTnc R3-WTnc

0,0306 0,0016 0,0206 0,0283

Unpaired t-test Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

CCL4 2w WT-WTnc C8-WTnc C8R3-WTnc R3-WTnc

p values 0,0007 0,0025 0,0031 0,0188

Unpaired t test with Welch's correction Mann Whitney test Unpaired t test with Welch's correction Unpaired t test with Welch's correction

CCL4 8w WT-WTnc C8-WTnc C8R3-WTnc R3-WTnc

p values 0,0001 0,0014 0,0073 0,0001

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

F4/80 C8-C8R3 C8-R3

p values 0,0116 0,0159

Unpaired t test with Welch's correction Mann Whitney test

TNF 2w WT-WTnc C8-WTnc C8R3-WTnc

p values 0,0104 0,0043 0,0043

Mann Whitney test Mann Whitney test Mann Whitney test

TNF 8w WT-WTnc C8-WTnc C8R3-WTnc R3-WTnc C8-R3

p values 0,0181 0,0151 0,0001 0,0005 0,0261

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test Unpaired t test Unpaired t test

TGF- β 2 2w C8-WT C8-C8R3 C8-R3 C8-WTnc

p values 0,0139 0,0124 0,0138 0,0151

Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction Unpaired t test with Welch's correction

TGF- β 2 8w C8-C8R3 WT-C8R3 WT-WTnc C8-WTnc

p values 0,0159 0,0079 0,0043 0,0043

Mann Whitney test Mann Whitney test Mann Whitney test Mann Whitney test

MCP-1

p values

DMSO-SP6

0,0055

Unpaired t test with Welch's correction

MCP-1 DMSO-SP6

p values 0,01

Unpaired t test with Welch's correction