PMA-5S csfB yaan yaa mno... USB yahoo divic daca. yabÐ yabc yaat yaba yazA yabr yaad yaa E sers. KSGA. mmV. gyrA yaaj dnax. A-165. MMA-235 yabyabe.
Table S1. Primers used for the construction of plasmids used in this study
a
Primer
Sequence a
Location on the genome b
dnaA.f
cgcgaattcTTATTACAGATATCACACCG c
1215...1234
dnaA.r
gcgcccgggTTTAAGCTGTTCTTTAATTTCTTTTAC
1747...1721
spo0J.f
cgcgaattcAGCGTGAAGATTTATCTCCG
4204583...4204603
spo0J.r
gcgctcgagTGATTCTCGTTCAGACAAAAG
4205088...4205069
1dnaA-HiF
cgcaagcttGATCTAGAAACGAGAATTGC
1238...1257
1dnaA-LinkR
tgcgatcctctcatttcaagTTTAAGCTGTTCTTTAATTTC
1747...1727
2dnaA-HisF
caccaccaccatcatcaccatTAGCAGGACCGGGGATCAATC
1748...1768
2dnaA-BaR
gcgggatccTCGTCAAATACTGATTTTGG
2251...2232
Linker-12His
cttgaaatgagaggatcgcatcaccatcaccatcaccaccaccatcatcaccat
Capital and lowercase letters indicate sequences derived from the B. subtilis genome and others, respectively. b Numbers refer to the SubtiList database (http://genolist.pasteur.fr/SubtiList/index.html). c Underlined sequences represent the recognition sites of restriction enzymes.
Table S2. Genes up- or downregulated by DnaA gene a
x1/5 DnaA b
x5 DnaA c
ratio
function
downregulated dnaA (dnaAN) d 0.65 0.39 0.13 replication 0.48 0.18 0.38 replication dnaN (dnaAN) d ykuN (ykuNPO) 1.58 0.73 0.46 similar to flavodoxin ykuO (ykuNPO) 1.84 0.73 0.40 unknown ykuP (ykuNPO) 2.22 0.89 0.40 similar to flavodoxin dhbA (dhbACEB) 1.04 0.59 0.57 biosynthesis of siderophore group nonribosomal peptides 0.70 0.34 0.49 unknown ywlC 0.70 0.45 0.64 unknown yydA noc (thdF/gidA/gidB/noc) 2.78 1.76 0.63 cell division gidB (thdF/gidA/gidB/noc) 3.11 1.70 0.55 translation gidA (thdF/gidA/gidB/noc) 2.10 1.16 0.55 translation thdF (thdF/gidA/gidB/noc) 1.77 1.00 0.56 translation upregulated 1.17 3.52 3.00 sporulation sda d yveL (yveKL) 0.19 0.56 2.95 similar to capsular polysaccharide biosynthesis protein yveK (yveKL) 0.24 0.65 2.71 similar to capsular polysaccharide biosynthesis protein yvdD (yvdDyvdC) 1.01 2.88 2.85 unknown yvdC (yvdDyvdC) 1.28 3.60 2.81 unknown protein with MazG nucleotide pyrophosphohydrolase domain 0.34 1.97 5.79 arginine catabolism rocA rocD (rocCDE) 0.76 2.06 2.71 arginine and proline metabolism a Genes that are downregulated (2.5) in a DnaA-dependent manner in two independent experiments are specified. The operon structure is indicated in parentheses. b Expression levels in NIS2022 cells grown in the absence of IPTG to repress the DnaA level to 1/5 times that in the wild-type strain, CRK6000. Expression level is the average of signal intensities of probes in coding sequences after trimming two of the lowest and highest intensities. c Expression level in NIS2022 cells grown in the presence of 0.1M IPTG to stimulate the DnaA level to 5 times that in the wild-type strain, CRK6000. d Expression levels of dnaA, dnaN and sda were calculated using probes in regions 168..333, 1751..1938, and 2646601..2646849, respectively.
840 210
420
630
1020 680
0
340 0
Cell Counts
1360
1050
1700
Figure S1
0
200
400
2 4
600
8
800
1000
0
200
400
2 4 origin/cell
600
800
8
1000
40,000bp
60,000bp
80,000bp
Spo0J
DnaA
20,000bp
dnaA
dnaN
recF yaaA
gyrB yaaB
gyrA
rrnO-16S
rrnO-23S
guaB rrnO-5S
dacA
yaaD yaaE serS
dck dgk yaaH
yaaC
recR bofA yaaK yaaL rrnA-16S
yaaJ dnaX
rrnA-23S
xpaC rrnA-5S csfB yaaN
holB yaaR yabB yabC tdk yaaQ yaaT yabA yazA
yaaO
yaaI
metG
yabD yabE
ispE ksgA sspF spoVG rnmV yabG veg purR yabJ gcaD
mfd ctc spoVC yabK
prs
yabM spoVT
yabN
yabQ yabP divIC yabO yabR
spoIIE
yabT yabS yacA
ftsH hprT
yacB
hslO
yacD cysK pabB
pabC pabA sul
folK yacF folB yazB lysS
rrnJ-16S rrnJ-23S
rrnW-16S rrnW-23S
abrB
140,000bp
160,000bp
180,000bp
Spo0J
DnaA
120,000bp
rrnW-23S
ctsR mcsA rrnW-5S mcsB clpC
radA
yacK
yacL
ispF gltX
cysS cysE
ispD
nusG yacO sigH secE rplK rplL yazC yacP rpmG rplA rplJ ybxB rpoB
rpsL fusA ybxF rpsG
rpoC
tuf
rplV rpmC rplX rpsH rpsE secY rplC rplW rpsC rpsQ rplE rplF rpmD ybaC rpsJ rplD rplB rpsS rplP rplN rpsN rplR rplO
adk map
infA rpsK rpsM rpoA ybxA ybaF rpsI cwlD rpmJ rplQ ybaE truA rplM ybaJ ybaK ybaL
kbaA
gerD
240,000bp
rrnI-23S
rrnH-16S rrnH-23S
rrnG-16S rrnG-23S
ybaR rrnG-5S
ybbM sigW ybbP ybbR
ybaS
feuC ybbA
ybaN
260,000bp
feuB feuA ybbB
ybbC
ybbD
ybbE
ybbF
ybbH ybbI
ybbT
ybbK ybbJ
280,000bp
Spo0J
DnaA
220,000bp
rrnI-16S
glmS
ybbU
adaB adaA ndhF
ybcC ybcD
ybcI ybcH ybcF
ybcL
ybcP ybcO ybcM
ybcT ybcS ybdAybdB
ybdE ybdK ybdD ybdG ybdJ ybdL
alkA
ybdO
ybxG
ybxH csgA
ybdMybdN
cypC
ybxI
ybfA ybeF ybfB
ybeC
ybyB
purT
ybfF ybfE ybfG
glpQ glpT
ybgB ybgA ybgE
ybfP ybfQ
ybfH ybfI
gltP
gamP
340,000bp
gamA
ycbA
ybgF
mmuM
ybgH
ycbB
ycbC ycbD
ycbE
ycbF
ycbGycbH
ycbJ
ycbK ycbM yczA ycbL ycbN ycbO
ybgJ
ycbR cwlJ phoD
ycbP
360,000bp
tatCD tatAD
ycbU
pcp
yccC
lmrB
lmrA
lip
natB natA
yccF
yczC
yccK
yccG yccH
ycdA
380,000bp
Spo0J
DnaA
320,000bp
ybfK ybfM ybfO mpr ybfJ pssA psd ybfN
ycdB
ycdA
ycdC
rapJ
ycdD
ycdFycdG
ycdH
ycdI yceA
yceD yceF yceC yceE yceG
yceB
yceH
opuAC opuAA opuAB
yceI
yceJ
yceK
amhX
ycgA
amyE ycgB
ldh
ycgF ycgE ycgG
lctP
mdr
ycgH
ycgI nadE
tmrB
aroK
ycgJ
ycgK cah
ycgL ycgM ycgN
ycgO
ycgP
ycgT
ycgR ycgQ ycgS
nasA
nasF
Figure S2-1
nasD nasE
nasC
nasB
yckD yckC yckE
yciA yciB yciC
yckB yckA
hxlR srfAA
nucA nin tlpC
hxlB hxlA
srfAB
comS
srfAC
440,000bp
460,000bp
480,000bp
Spo0J
DnaA
420,000bp
ycxA srfAD
srfAC
yclC yclB
ycxD
ycxC ycxB
yckK
sfp yczE yckI yckJ
yclDyclE
yclA
yclG
yclF
gerKA
gerKC gerKB
yczF
yclJyclK
yclH
rapC
phrC
yclI
yclN yclO yclP yclQ
yclM
yczG
ycnB
gabT
gabD
ycnE ycnC ycnD gabR
ycnJ ycnI
mtlD
ycsA
sipU
ycnK
ycsD
ycsE
ycsF ycsG
ycsI kipI kipA
kipR ycsKyczI pbpC
yczH
ycsN mtlR
yczJ
540,000bp
ydaE ydaG ydaD ydaF ydaH
ydaB
ydaC
lrpC topB
ydaJ ydaK ydaL
ydaM
ydaN
ydaO
ydaP mutT
ydzA
mntH
560,000bp
ydbB ydbA gsiB ydbC
ydaQ
ydaT ydaS
dctS
dctR dctP
ydbD dctB
580,000bp
Spo0J
DnaA
520,000bp
mtlA ycnL
glcU gdh
dctP
ydbI
ydbL ydbJ ydbK ydbM
ydbO
ydbN
ddl
murF
ydbT ydbS
ydbR
ydbP
ydcC acpS
alr
rsbR rsbW ydcG ydcE rsbT sigB ydcH ydcD rsbS rsbU rsbV rsbX ydcF ydcI
ydcO ydcQ sacV ydcP
ydcK
ydcA
ydcL
ydcR
ydcT yddD yddA yddE ydcS yddB yddC
yddG yddF
yddH
yddI yddJ
ydcM ydcN
rapI
yddM phrI
yddK
yddN
yddQ yddR yddS
yddT
ydeAcspC
ydeD
ydeC ydeB ydzE
lrpB
640,000bp
ydeF
ydeG
ydeI ydeH
ydeE
ydeL
ydeM
ydeO
ydzF ydeN
ydeJ ydeK
ydeQ
ydeP
ydfA ydeS ydeT
ydfB
ydeR
660,000bp
ydfD
ydfC
ydfF
ydfE
ydfH
ydfO ydfQ ydfN ydfP
ydfI ydfJ
ydfG
nap
ydfK ydfL ydfM
ydzH ydfR
680,000bp
Spo0J
DnaA
620,000bp
lrpA
ydfS
ydfR
ydgD ydgC ydgE
ydgB cotP ydgA
ydgH ydgG
expZ
ydgF
ydgK
ydgJ ydgI
dinB
ydhCydhD
ydhB
ydhE
ydhG
ydhI ydhH
ydhFphoB
ydhO ydhN ydhM
ydhJ ydhK
ydhP
ydhR ydhQ ydhS ydhT
ydhL
ydiC gcp rrnE-5S ydiB ydiD
rrnE-16S rrnE-23S
ydhU
ydiH tatCY moaC tatAY
ydiM
ydiN ydiO
ydiP
ydiQ
ydiR ydiS
ydjA
ydiL ydiK
ydiF
ydjBydjC
gutB
gutP
ydjE
ydjG pspA ydjH ydjI
gutR
740,000bp
ydjN ydjM
ydjK
ydjJ
ydjP ydjO yeaA cotA
ydjL
760,000bp
yeaB yeaC
yeaD yebA
guaA
pbuG
yebE purE yebC yebD yebG purK
purB
gabP
780,000bp
Spo0J
DnaA
720,000bp
groEL groES
purQ purB purC purS purL
purF
purH purM purN
purD
yecA
yezC
yerA
yerB yerC
yerD
pcrB pcrA
ligA
yerH
gatA gatC
yerI
opuE sapB
gatB
yerP
yerO
yerQ
yeeA yefC
yefA
yefB
yeeB
yeeC
yeeG
rapH
yeeI
cotJC yeeK yesE cotJB yesJ yesL yezE yesF cotJA yesK yesM
yezA yeeD yeeF
Figure S2-2
yesN
yesO
yesP yesQ yesR yesS
yesT yesV yesU yesW
yesX
yesZ yesY
yetA
lplA
lplB
lplC lplD
yetF
yetI
yetH yetG
yetJ yezD yetK yezB
yetM
yetL
yetO
yetN
yfnI
yfnH yfnG yfnF
840,000bp
860,000bp
880,000bp
Spo0J
DnaA
820,000bp
yfnF yfnE
yfnD
yfmT
yfnC
yfmP yfmQ yfmO
yfmS yfmR
yfnB yfnA
yfmN
yfmL
yfmM
pel yflT yfmA
yfmG yfmH
yfmK
yfmJ
yfmI
yflS
citS
citT yflP
citM
yflN
yflM
yfmFyfmE yfmD yfmC yfmB
yflK
yflL
yflC
yflI yflG yflJyflH
yflE
treP
yfkT
yfkR yfkS
treA
treR yfkO
yfkQ
yfkI yfkJ yfkH
yfkM
yfkN
yfkL
940,000bp
yfkK
yfkE
yfjS yfjT
yfkD
yfkA yfkC yfkB
yfkF
yfjP
yfjR
yfjO
yfjN
yfjL yfjM
acoA acoB acoC acoL
acoR
sspH
malA
yfjE yfjC yfjF yfjD yfjB
yfjQ
960,000bp
yfiA malP
yfiB
yfiC
yfiDyfiE
yfiF
yfiG
yfjA
980,000bp
Spo0J
DnaA
920,000bp
yflD yflA yflB
nagP
yfiG
yfiH
yfiI
yfiJ
yfiK yfiL
yfiM
yfiN
lipB yfiQ
yfiT
yfiS yfiR
padR
yfiU
yfiWyfiX
yfiZ
yfiV
yfhA
yfhB yfhC
yfhG yfhH yfhI
yfhF yfhE yfhD
yfiY
yfhK yfhM yfhJ yfhL csbB
yfhO
yfhQ
sspK
yfhP
fabL
sspEygaC ygaB ygaD
yfhS
ygaE
gsaB
rrnD-16S rrnD-23S
ygaJ ygzA thiC
rrnD-5S
ygzB
senS
spo0M
ygaK
1,040,000bp
ssuB
ssuA
ssuCssuD
yhzA ygaN
katA
yhbA
yhbD yhbF yhbB cspR yhbE prkA
yhbH
yhbJ yhbI yhcA
yhcE yhcC yhcF yhcH yhcB yhcD yhcG yhcI
ygaO yhzB
yhcJ
cspB
1,060,000bp
yhcL
yhcK
yhcO yhcN yhcP
yhcM
yhcR
yhcS
yhcQ
yhcV yhcX yhcU yhcW
yhxA
yhcT
1,080,000bp
Spo0J
DnaA
1,020,000bp
perR ygaF ygxA
yhxA
glpF glpP glpK
glpD
yhxB
yhcY
yhdA yhcZ yhdC
lytF yhdB
spoVR
ygxB yhdE
lytE
phoA
citA
yhdF
yhdG
yhdH
citR
yhdJ
yhdI
yhdN yhdO
yhdL yhdK sigM
yhdP
yhdR
yhdQ
yhdY yhdX yhdZ
yhdT
ccrB ccrB yhdW
yhdS
yheN
nhaC
yheH
yheB
sspB yheF yheD yheG yheE
nhaX
yheC
yheA
yhaZ
yhaX hemZ
yhaY
1,140,000bp
yhaR yhaQ yhaP
yhaU
yhaO
yhaN
yhaM yhaL
yhaT yhaS
prsA
ecsB ecsA
yhaI
yhaJ yhaG yhaK hpr yhaH serC
hit
1,160,000bp
ecsC
pbpF
yhaA
yhfA
hemE
hemH hemY
yhgE yhgD
yhfC
yhgC yhgB
fabHB
yhfE yhfF
yhfD
gltT
yhfI yhfJ
yhfK
yhfH
1,180,000bp
Spo0J
DnaA
1,120,000bp
yheI yheJ
dat
yhfL yhfK
yhfP yhfO yhfQ
yhfN
yhfM
aprE
yhxC
yhfS yhfR yhfT
hemAT yhfU yhfW
comK
yhzC yhxD
yhjA
yhjB
sipV yhjE yhjG
yhjD yhjC
yhjH
yhjM
glcP
yhjJ
yhjK yhjL
yhjP
yhjN
yhjO
addB
yhjR yhjQ
addA
sbcD yirY
yisB
yisK
yisL wprA
gerPD gerPA gerPF gerPC yisI gerPE gerPB yisJ
Figure S2-3
asnO
yisN
yisP
yizA
yisR degA
yisQ
yisS
yisV
yisU yisT
yisY
yisX
yitC yitD
yitA yisZ
yitB
yitL
yitE yitF
yitG
yitH yitI yitJ
yitK
yitQ
yitN yitP yitM yitO
yitR nprB
yitT ipi
yitS
yitV
yitU
yitW yitY
yitZ argC argJ
argB argD
carA carB
1,240,000bp
1,260,000bp
1,280,000bp
Spo0J
DnaA
1,220,000bp
carB
argF yjzC
yjaU
med fabHA yjaV comZ fabF
yjzD
yjaZ appD appF appA
appB appC yjbA
yjzB
oppA
oppB oppC oppD oppF yjbB
yjbF mecA
yjbC spxA
trpS
yjbM yjbL ppnK yjbO
yjbG
yjbE
yjbH yjbI yjbJ yjbK
yjbV fabI yjbX
yjcA
yjcB yjcC
cotZ cotX cotV cotY cotW
yjbP
yjcI
yjcD
yjcJ
yjcE yjcG yjcF yjcH
1,340,000bp
trnSL-Val2
yjcL yjcK
yjcQ yjcP yjcS yjcR fabG
yjcN yjcO
yjcM
manR
manP
manA yjdF
yjdI
yjdH yjdG
yjdB
1,360,000bp
yjeA
yjdJ ctaO
yjfC
cotT
yjhA
yjgC
yjgD
yjgA yjgB
yjfAyjfB
yjhB
yjiB
yjiC
yjlD yjlB yjlC
yjjA
yjiA
uxaC
yjkA yjkB yjlA
1,380,000bp
Spo0J
DnaA
1,320,000bp
tenI thiG tenA goxB thiS thiF
yjbQ
uxaC
yjmB
yjmC yjmD uxuA yjmF exuT
exuR uxaB
uxaA
yjoB
yjnA
rapA
yjoA
xlyB phrA
xpf xkdC xtrA xtmB xkdB xkdD xtmA
yjqByjqC
yjpA
xkdE
xkdFxkdG
xkdI xkdK xkdH xkdJ
xkdS xkdQ xkdT xkdP xkdR xkdUxkdV
xkdN xkdM xkdO
xepA xlyA xkdX xhlB xkdW xhlA
ykcA
pit spoIISA spoIISB
xre xkdA
yjqA
ykcC
ykaA ykbA
proG dppA dppB dppC dppD dppE
ykfAykfB
ykfC ykfD
htrA
1,440,000bp
ykhAhmp
ykgB
ykgA
ykjA
ykkC ykkA ykkD ykkB ykkE
proB proA
ykzH
yklA
ykzA
ykmA
1,460,000bp
ykoH ykoG
ykoB
metE guaD
ispA
ykoC ykoD
ykzD ykoI ykoJ ykoK
ykoF ykoE
ykzB ykoM ykoL
tnrA
ykoP
ykoN
1,480,000bp
Spo0J
DnaA
1,420,000bp
ykcB
ykoP
ykoS
ykoQ
ykoY ykoX
ykoT
ykoU
sigI ykrI
ykrL
ykrM
ykrK sspD
ykoV ykoW
ykzE
ykrP
kinE
ogt
ykrV
ykrS
ykrT
ykrU
ykrW
ykrY ykrX ykrZ
eag spo0E
ykvA
ykvE
kinD
ykvJ ykvL ykvK ykvM
ykvI
motA motB clpE
ykvP
ykvQ
ykvN
ykvR
ykvT ykvU
ykvV ykvW
ykvY
ykvZ glcT
ptsI ptsH
ptsG
splB splA
ykvS
mcpC
ykwC
ykwB
1,540,000bp
ykuA
kinA
ykwD
cheV
ykuF ykuG
ykuC ykyB
patA
ykuI ykuH
ykuK ykuL ykuO ykuQ ykuJ ykzF ccpC ykuN ykuP ykuR ykuS
ykuE ykuD
1,560,000bp
ykuT
ykuV ykuU rok
moeB moaE mobA moeA mobB moaD
yknT
1,580,000bp
Spo0J
DnaA
1,520,000bp
ykvO
yknU
yknV
yknX yknW yknYyknZ
fruR fruK fruA
sipT
ykpA
ykoA
kinC abh
ykpB
ampS
mreBH ykpC
ykqA ykqB adeC
ykrA
ykqC
ykzG
ykyA pdhA pdhB pdhC
def
pdhD
yktA
slp
speA
yktB
yktC ykzI ykzC
yktD
ylaA
nprE
ylaC ylaB ylaD
ylaG
ylaE ylaF
ylaH
ylaJ ylaI
ylaK
ftsW ylaM ylaN
ylaL
Figure S2-4
pycA
ctaB
ctaA
ctaC ctaD
ctaF ctaE ctaG
ylbC ylbB
ylbA
ylbF ylbE ylbH ylbD ylbG ylbI
ylbJ
rpmF ylbN ylbO
ylbK ylbL
ylbM
ylbQ yllA
ylbP
yllB ftsL mraW pbpB
spoVD
murE
mraY murD
ylxW ftsA spoVE murG murB divIB ylxX sbp
ftsZ
bpr
1,640,000bp
1,660,000bp
1,680,000bp
Spo0J
DnaA
1,620,000bp
sigE spoIIGA sigG ylmA ylmB
bpr
ylmF ylmD ylmG divIVA ylmC ylmE ylmH ileS
lspA ylyA ylyB
pyrP pyrR
pyrB pyrC
pyrAA carB
pyrK pyrD pyrFpyrE cysHcysP
sat
ylnD cysC ylnE ylnF
yloB
yloC
ylzA yloI gmkrpoZ
priA
fmt def
yloM
yloN prpCprkC
yloQ rpe
yloS
yloA
recG sdaAA sdaAB
plsX ylpC
rnc fabD fabGacpP smc
ftsY
rpmB
1,740,000bp
ylqD rpsP trmD ylqC rimM rplSylqF rnhB ylqG
ffh ylxM
sucC ylqH sucD smf
topA
gid
clpY codV clpQ
flgC fliF codY flgB fliE
fliG
fliH fliI
fliJ
ylxF
fliK
flgE ylxG
ylqB
1,760,000bp
1,780,000bp
Spo0J
DnaA
1,720,000bp
yloU yloV spoVM
flgE fliLfliM
fliY
fliZ fliR cheY fliP fliQ flhB
flhA
flhF
ylxH cheB cheA
cheC sigD rpsB cheW cheD ylxL tsf
frr pyrH
cdsA uppS dxr
yluC
proS
nusA ylxS
polC
ylxR infB ylxQ
rbfA ylxP truB ribC
pnpA rpsO
ylxY mlpA
spoVFA asd ymxH spoVFB
dapG
dapA ymfA
tepA spoIIIE
ymfC
ymfD ymfF ymfH ymfE ymfG
fabG
ymfL ymfJ ymfM cinA ymfK pgsA
recA
pbpX
ymdA
tdh ymdB spoVS
kbl
ymcB
cotE ymcA mutS
mutL
pksApksB pksC
pksF acpK
pksD pksE
pksG
pksI pksH pksJ
ymcC ymzD
1,840,000bp
1,860,000bp
1,880,000bp
Spo0J
DnaA
1,820,000bp
pksL
pksM
pksN
pksR
ymaCymaD
pksS
1,940,000bp
ebrA ebrB ymaG
ymaH ymaA ymzC nrdE ymzA
nrdF ymaB
spoVK ynbA ynbB
glnA glnR
ynxB ynzG ynaC ynaE ynaG ynzF ynaB ynaD ynaF ynaI
cwlC
xynP
xynB
xylA
xylR
1,960,000bp
xylB
yncF yncE
yncC
yncB
yncD
1,980,000bp
Spo0J
DnaA
1,920,000bp
ymaE ymzB aprX
ymaF miaA
yndD yndA ynzB
thyA
ynzH
yncM
cotC yndB tatAC
yndE yndF
yndH yndG yndJ
yndL yndK
yneB tkt yneA ynzC
yndN
yndM
lexA
yneF yneI yneE ccdA yneJ
ynzD
citB
sspP cotM yneK sspO
sspN tlp yneQ yneP
yneN
yneTparE
yneS yneR
parC
alsT
ynfC
bglC
yngB yngA yngC
ynfE
ynfF
xynD
yngD
yngE
yngFyngG yngH
Figure S2-5
yngI
yngJ
yngK ynzE
ppsE yngL
ppsD
ppsC
ppsB
ppsA
dacC
yoxA
2,040,000bp
2,060,000bp
2,080,000bp
Spo0J
DnaA
2,020,000bp
yoeB
ggt
yogA
yoeD yoeC
yoxA yoeA
yofA
yoxB yoxC yoaA
gltC
gltB
gltA
proJ
proH rtp fabG
yoaE
yoaB
yoaC
yoaF
yoaD
yozQ
yoaG
yoaI
yoaH
pelB
yoaJ yoaK
yozG yoaS yoaT
yoaQ
yoaN
yoaP yoaO
yoaR yozF
yoaV
penP
yoaZ yoaW
yoaU
yobE yobD yozI
yobB
yobA
pps
xynA
yozH
2,140,000bp
rapK phrK
yobF
yozM
yobN
yozK yobH yozL yobI
yozJ
yobL yobJ yobK
yobO
yobQ yobS yobU csaA yobR yobT yobV
yobM
2,160,000bp
yocD yocC des
yobW yocA
yozA
yocN yozN
yocF yocG
yozB yocB
dhaS
yocK yocM acpD yocL yozO
yocH yocI
2,180,000bp
Spo0J
DnaA
2,120,000bp
yoaM
dhaS
sqhC
sodF
yocS
yocR
yojI
odhB
odhA
yojO
yojN
yojM yojL
yojK
yojH
yozR
yojF yojG yojE
yojJ
yodA
yojB yojA yojC
yodC
yodB
yodI yodH
yodF
yodD yodE
cgeB cgeA
yodM yodN kamA yodJ deoD yodL yozD yozE
ctpA
yodS yodR yodT
cgeD cgeE
cgeC
phy
yotL
sspC
yotE yotH yotD yotJ yotC yotM yotIyotG yotB yotN yotK yotF
yosU
yosX yosP yosW yosS yosQ yosZ yosV yosT yosR
2,240,000bp
ligB yoqW
yosA
yosJ yosF yorY yosM yosI yosE yorX mtbP yosL yosH yosD yosB yorV yosK yosG yosC yorZyorW
yosN
yorP yorS yorO yorR yorN yorL yorT yorQ yorM
2,260,000bp
yorK
yorJ
yorI
yorG yorE yorC yorA yorH yorF yorD yorB
yoqZ
yoqY yoqX
yoqT
yoqO yoqM
yoqH yoqE yoqSyoqP yoqL yoqJ yoqG yoqD yoqU yoqR yoqN yoqK yoqI yoqF
2,280,000bp
Spo0J
DnaA
2,220,000bp
yodPyodQ
yodU
yonO yonP
yopS
yopZ yopV yopY yopT yoqA yopX yopU yoqB yopW yopR yopQ
yopM yopN yopK yopP yopO yopL
yopH yopE yopI yopG yopC yopJ yopF yopD
yopA yopB
yonV yonX
yonN
yonJ yonK
yonT yonR yonU yonS
yonG yonH yonF
yonE
yonD
yomX yonB yomZ yomW yonC yonA yomY yomVyomU
yomR yomO yomS yomP yomM yomT yomQ yomN
yomJ yomK yomI
yomG yomH
yomE yomF
yomD blyA
yomL yozP
yonI
yolJ bdbB
2,340,000bp
yolC
sunT bdbA
yolF sunA uvrX
yokF yokEyokD
yolA yolD yolB
yokK yokI yokL yokJ
2,360,000bp
yokA
yokB yokC
yokH yokG
ypoP
msrA yppQ ypnP
yplP yplQ
ypmS ypmQ ilvA ypmTypmR ypmP
ypkP thyB ypjQ ilvD dfrA ypjP ypiP yphP
2,380,000bp
Spo0J
DnaA
2,320,000bp
bhlB bhlA
metA ugtP
ilvD
ypgR
ypgQ bsaA
ypzA cspD
ypdQ ypdP sspL
ypeQ degR ypeP
ypcP
ponA ypqA yppF sspM recU
kduI kduD
ypbR ypbS
bcsA ypbQ
pbuX
xpt
ypwA
kdgK kdgT kdgA kdgR
ypvA
ypsC yptA
ypsA yprB ypsB cotD
yprA
yppE yppC ypqE yppG yppD
ypjC
nth asnS ypoC dnaD
aspB
ypmB dinG ypmA
panC panD panB birA cca
Figure S2-6
ypjH
ypjG dapB mgsA ypjD
ypjB
ypjA
qcrC
qcrB ypiB qcrAypiF ypiA
aroE
tyrA
hisC trpA trpB
trpF trpC trpD trpE
aroB aroH
aroF
cheR ndk hepT
menH mtrB hbs hepS folE spoIVA
yphF
yphE gpsA yphC
seaA
yphA ypgA ypfD
ypfB cmk ypfAypeB
sleB
ypdC
2,440,000bp
2,460,000bp
2,480,000bp
Spo0J
DnaA
2,420,000bp
fer
ypbD ypbH ypbE ypbG ypbF recQ
ypdA ypdC gudB
serA
ypzE ypaA
ypbB
ypuF
aroD
rsiX
sigX resE
resD
resC
resB
rluB spmA resA spmB dacB
ypuH ypuI scpA
ypzC
ribA ribTribH
ribD ribE
ypuD sipS
ypuE
ypzD ypuA
ypuB ypuC
ppiB
ansR
lysA
drm punA
xerD
fur
spoIIM mleA yqkK mleN
ansB
ansA
yqxK
2,540,000bp
nudF
yqjV yqzH
yqkD
yqkE
yqjU
yqkA yqkB yqjY yqjW yqkC yqjZ yqjX
proI
coaA yqjT dsdA
yqjP yqjQ
yqjM yqjL
yqjN
yqjK
2,560,000bp
yqjG yqzJ yqjF
zwf
yqjI
yqjH
bmrU bmr
yqjE
yqjD
bmrR
yqjB yqiY yqjC yqjA yqiZ yqiX yqiW
bkdB
bkdAA bkdAB lpdV
2,580,000bp
Spo0J
DnaA
2,520,000bp
spoVAA dacF spoVAD spoVAB spoIIAA spoVAF spoVAE spoVAC sigF spoIIAB
yqkF
yqzF
lpdV
buk
bcd
ptb
bkdR
yqiG
yqiQ prpD
mmgA mmgD mmgC mmgB yqiK yqiI yqiH
yqhQ yqhP
spo0A spoIVB recN
yqxC ahrC dxs
yqiD
yqiC yqiB
yqhY folD nusB accC
spoIIIAF spoIIIAD spoIIIAA spoIIIAH spoIIIAE spoIIIAB efp yqhR accB spoIIIAG spoIIIAC yqhV yqhT yqhS
yqhM yqhO mntR
gcvPB
sinI yqhG sinR
yqhH
yqzG
comGD comGG comGB comGFcomGC comGA yqzE comGE yqxL
yqxM tasA sipW
gcvPA gcvT
yqhB
2,640,000bp
yqzD yqzC
yqgY yqgW
yqhA yqgZ yqgX
yqgV yqgU yqgT
yqgS
glcK
yqgQ yqgP
yqgO rpmGA yqgN yqgM yqgL
2,660,000bp
yqfY yqfZ
yqgB
pstBA pstBB pstA pstC pstS pbpA
yqgE
sodA yqgC
yqgA
yqfW
yqfT
yqfX zur yqfU
yqfQ yqfP
yqfS yqfR
yqfO
sigA yqfN cccA
2,680,000bp
Spo0J
DnaA
2,620,000bp
yqhL
antE
sigA
yqeW
yqfL glyS yqxD yqzB
dnaG
glyQ
recO era
dgkA yqfF cdd yqfG
phoH yqfD
yqfB yqfC yqfA yqeZ
rpsU yqeY
rpsT
yqeV
yqeT yqeU dnaJ
dnaK
hrcA grpE
hemN lepA
spoIIP yqxA gpr
yqeN
comER
comEC
nadD yqeL yqeI yqeG yqeM yqeK aroE yqeH
yqeD
yqeF cwlH
yqeB
yqeC
yqzI rapE phrE yqcG
spoIVCB
nucB spoIVCA
yqcF
yqcD yqcB yqbS yqxI yqxG yqcC yqbT yqbQ yqbO yqxJ cwlA yqxH yqcE yqcA yqbR yqbP
arsB arsR arsC yqcK yqcI
2,740,000bp
yqdB
yqbN
2,760,000bp
yqaP
yqbH yqbE yqbL yqbI yqbG yqbC yqbM yqbK yqbJ yqbF yqbD yqbB yqbA
yqaT
yqaR yqaS yqaQ
yqaE
yqaN yqaL yqaO yqaM yqaK yqaJ
yqaI yqaF yqaC yqaG yqaH yqdA yqaD
2,780,000bp
Spo0J
DnaA
2,720,000bp
comEB comEA
sda
spoIIIC yqaB
yrkO
yrkR yrkQ
yrkS
yrkP
yrkM yrkN
yrkL yrkK
blt
yrkJ
yrkI yrkF yrkD yrkB yrkH yrkG yrkE yrkC bltR
bltD
yrkA
yrdQ
yrdR
gltR
trkA
czcD
yrdN
yrpB
brnQ yrdK
azlC cypA azlD azlB yrdF
yrdC yrdD yrdB
aadK yrdA
yrpC
yrpD
yrpG
yrpE sigZ
yraK yraL
yraM
yraO
yraN
csn
yraF yraD yraG adhB yraE
yraI yraJ yraH
Figure S2-7
yraB
adhA yraA
sacC
aapA
levD levG levF levE levR
yrhO
yrhP
sigV yrhM yrhL
yrhK
yrhJ
yrhE
yrhG yrhI yrhH yrzI yrhF
yrhD
yrhB yrhC
yrzA
yrhA mtn yrrT
yrrS yrrR
udk greA yrrO
yrrN yrrM yrrL
yrzB yrzL yrrK alaS
2,840,000bp
2,860,000bp
2,880,000bp
Spo0J
DnaA
2,820,000bp
glnQ glnH
alaS
glnM glnP
yrvN
yrrC yrrD
yrrI
yrrB trmU
yrvO
yrzC
yrvJ
yrvM
aspS
hisS
yrzK
spoVB
relA yrvI
apt
yrvE
yrvC yrvD secDF
yrzD
yrzG yrbD yrzF yrzH
yrzE
yrbG
tgt yajC
csbX ruvB ruvA bofC
queA
yrbE
yrbC
coxA safA
nadA
yrxA
nadC nadB
ysnE ysnD ysnF
ysxD
pheA
pheB obg
rpmA ysxB spoIVFB minD mreD radC spoIIB folC rplU spoIVFA minC mreCmreB maf comC
spo0B
2,940,000bp
valS
ysxE spoVID
hemL
hemB
hemD hemX hemC hemA
2,960,000bp
lonA ysxC
lonB
clpX
tig
ysoA
leuD leuC
leuB
leuA
ilvC
ilvB ilvH
ysnB ysnA
2,980,000bp
Spo0J
DnaA
2,920,000bp
nifS
yslB
rph ysnA gerM
gerE sdhB racE ysmB ysmA sdhA
sdhC
ysfB sspI
rnhC
lysC
uvrC ask
xsa trxA
mutSB yshE
etfA etfB ysiB ysiA lcfA
yshA yshB
yshC
pheT
pheS
ysfC
ysfD
yscB yscA
ysdB
cstA ysfE
ysgA
araQ
araP
araN
araM araL
araD araB
araA
abnA ysdC
rpmI rplT ysdA infC
lytT ysbA ysbB lytS
3,040,000bp
ytbD
dnaI ytxC ytxB dnaB
thrS
gapB ytcG speD
ytbE
ytvI
ytaF polA ytaG mutM
ytcD
phoR
phoP mdh icd
3,060,000bp
citZ
ytrI
pyk ytzA
ytwI
pfkA
accA accD ytsJ
dnaE
ytpI
ytqI
ytoI
ytnM ytnL
3,080,000bp
Spo0J
DnaA
3,020,000bp
abfA
ysaA
ytlI
ytnL
ribR
ytnJ
ytmO ytmM ytmK ytmI ytnI ytmN ytmL ytmJ
ppnK
argH ytkL fabG ytzD
argG
ackA moaB
ytxK
ytfI sppA tpx ytfJ yteJ
braB
ytcJ
ytcI
ytbJ sspA
nifZ
hisJ
ezrA
ytsP
yttP
acuB acuA acuC
rpsD
ytrP
tyrS
ytxEytxD ccpA
aroA
ytxH ytxJ ytxGmurC
ytpT
ytpS
ytzB
ytpR ytpP ytpQ ytoP
3,140,000bp
ytzH
malS
ytkP
ytnP ytmQ ytmP amyX
ytlR
ytlQ ytlP
ytiP
ytjP
ytzE
ythQ
ythP
ytfP
ytzG ytzF ytgP
3,160,000bp
opuD
ytdP
yteU yteV yteT
yteS yteR
yteP yteQ
msmR
ytcQ
ytcP
ytbQ bioI
bioB
bioD bioF
bioA
msmE
amyD
amyC melA
bioW ytaP
3,180,000bp
Spo0J
DnaA
3,120,000bp
acsA
ytoQ
melA
ytvA
leuS ytwF
ytvB
yttA
yttB
ytqA ytqB
ytsD
ytsC ytsB ytsA ytrF
ytrE ytrD ytrC ytrB ytrA ytzC
ytpB ytpA
ytoA
ytnA
ytlC ytlA ytlB ytlD
pckA
asnB
metK
ytmA ytmB
ytjA
ythA
ytkC ytkA dps luxS ytiB ytiA
ytkD
ythB ythC
mntD mntC
yteA
mntA mntB menC menE
ytxM menB menD
menF
Figure S2-8
ytdA
ytcA
ytcB ytcC
ytaA
cotS ytxO cotSA
ytaB
glgP
yuaJ
glgA
glgD glgC
glgB
rrnB-23S
rrnB-16S
yuaG yuaI
yuaE
yuaF
gbsB yuaD gbsA
yuaB yubG yuaC yuaA
yubC
yubE yubF yubD
uppP yubA
yulF
yulE
yulC yulD
3,240,000bp
3,260,000bp
3,280,000bp
Spo0J
DnaA
3,220,000bp
tgl
yulC
yulB yuxG
tlpB
mcpA
tlpA
yugO
yugT yugU
mcpB
yugS
yuzA
yugM yugN pgi
yugP
yugK
yugJ
yugIalaT
yugF
alaR
patB
kinB
kapB
yugE
yuxJ
pbpD
kapD
yuxK
yufL
yufM
yufN yufO
yufP yufQ
maeN
yufK
mrpD mrpC mrpB
mrpA
mrpG mrpF mrpE
comA yuxO comP
yufS
yuzE
comQ comX degQ yuxH
3,340,000bp
yueK
yueG yueI yueF yueJ yueH
yukF
yueE yueC yuzF yueD yueB
yukA
yukB yukC
ald
yukE yukD
yukJ yuxI
yuiG
dhbF
3,360,000bp
dhbB dhbE
dhbC
dhbA yuiI
yuiH
yuiD
yuiF
pepA
yuiC
yuiA yuiB yumB
3,380,000bp
Spo0J
DnaA
3,320,000bp
yuzC
yumC
yumB
guaC
yutJ
paiA dapF paiB yutM yutK
yuzG
yuzB
yutG
yutI
yuxL yuzD
thrB thrC hom
yutH
yutC
yutF
yunA
yutE yutD lipA
pucR
yunC yunB yunD
pucJ
pucK
pucL
pucM
yunF pucH yunE yunG
yurI
pucD pucE
pucA pucC pucB
yurH
yurJ
yurL yurM yurN yurO
yurP
yurR yurQ
yurT yurU
csd yurV
3,440,000bp
yurX
yurY
yusG yusE gcvH yusJ yusDyusF yusI
yusA yusC yurZ yusB
yusK
yusL
yusT
yusR yusQ yusS
yusM
3,460,000bp
yusZ mrgA
yusV yusX yusU yusW
yusY
cssS cssR
yuxN
yvtA
gerAA
gerAB gerAC
yvqE yvqC
fumC
yvqG yvqI yvqF yvqH yvqJ
yvqK
3,480,000bp
Spo0J
DnaA
3,420,000bp
yurG
yusO yusN yusP
yurS sspG
yurK
yvrD
yvrA yvqK
yvrB
yvrC
oxdC yvrL
yvrE yvrG
yvrH yvrI
yvgJ yvsG
fhuD
yvrN
yvrP yvrO
fhuC fhuG fhuB
yvsH
sspJ
yvgL yvgM
yvgK
yvgN
yvgO
yvgP
yvaO yvaQ yvaM yvaN yvaP
yvaA
yvgQ
yvgR
bdbD yvgTbdbC yvgW
yvgS
yvaE yvaF yvaD yvaG
acpDyvaC
rnr smpB
yvaX yvaW yvaY
opuBC opuBA opuBD opuBB
yvaK secG yvzC
3,540,000bp
yvbA opuCC opuCA yvaZ opuCD opuCB
yvbF
yvbI yvbH
yvbG
yvbK
yvbJ
eno
araR
pgm
3,560,000bp
tpiA pgk
gapA cggR
araE
yvbV
yvbT
yvbU
rsbQrsbP
yvbW
yvbX yvbYyvfW
yvfV yvfUyvfT
yvfS yvfR
3,580,000bp
Spo0J
DnaA
3,520,000bp
yvgZ yvgY
yvgX
yvaV
rsbP
yvfH
yvfO
lacA
yvfM yvfL
yvfK
lacR
yvfI
yvfG
sigL
yvfF yvfE
slr pnbA
yvfB yvfDyvfC
yveT yvfA yveS yveR yveQ yveP
yveN yveO yveM
yveLyveK
sacB
racX yveG padC yveF pbpE
yveB
yveA
yvdS yvdT yvdR
yvdP yvdQ
yvdO
clpP
yvdC
malL pgcM
yvdK
yvdJ yvdI yvdH
Figure S2-9
yvdG
yvdF
yvdE
yvdD yvdB
yvcT
yvdA
yvpB yvpA
yvcS
crh yvcR yvcQ yvcP yvcN yvcL yvcK yvcJ yvcI trxB
yvcE
yvcD
yvcC
yvcB yvzA
yvcA hisI hisF hisA
hisH hisD hisB
hisZ hisG
hprP yvoF yvoD lgt
nagA
hprK
nagB yvoA
yvnB
3,640,000bp
3,660,000bp
3,680,000bp
Spo0J
DnaA
3,620,000bp
yvmA yvmB
yvnA
yvnB
cypX
yvkN
yvlB yvlC yvlD
yvmC
uvrA yvlA yvzB
uvrB
yvhJ yvyE
yvkA yvkB
yvkC
csbA
yvjD
yvzD
yvjB
ftsX ftsE
yvjA cccB prfB
secA
fliS fliT fliD
yvyD
hag yvyC
yviF yviE csrA flgL flgK
degS yviA degU
tagO
tuaH
tuaG
tuaF tuaE
tuaD
tuaC
tuaB
tuaA lytC
3,740,000bp
lytB
lytA
gtaB
yvyH
tagA tagB
ggaB
ggaA
tagH
tagG tagF
tagE
3,760,000bp
tagC
tagD
gerBA
lytD
gerBB gerBC
pmi
ywtF ywtE
ywtG
ywtD
ywtC ywtB
ywtA ywsC
3,780,000bp
Spo0J
DnaA
3,720,000bp
comFC flgM comFB yvyG yvyF comFA
lytR
rbsA rbsR rbsK rbsD
ywsA rbsC rbsB ywsB
alsR
ywrK
alsD ywrO alsS
cotG ywrFywrE
cotB ywrJ
cotH
ywrB ywrC ywrA
ywqM
ywqN ywqO
ywrD
ywqB
ywqK ywqL ywqJ
ywqH ywqI ywqG ywqF
ywqE
ywqA
ywpF
ywqD ywqC
ywpD
ywpG ywpJ glcR ywpH ywpE
ywpB mscL
spoIIID usd
flhP flhO mbl
nrgA
ywoF
ywoE
ywoD
ywoA nrgB
ywoB ywoC
ywnG ywnH
spoIIQ ywnJ
3,840,000bp
ywnE
ywnF
ywnC
mta
ywmA
ureC ywnA ywnB
ureA ureB
ywmF csbD rapB
ywmD ywzB atpD moaA fdhD ywmE ywmC spoIID murAA ywmB atpC
3,860,000bp
ywkC ywkD
ywlA
atpG atpA
atpF atpB atpH atpE atpI upp glyA
ywlF ywlD ywlG ywlE ywlC
spoIIR ywkE ywlB ywkF prfA
ywkB ywkA
3,880,000bp
Spo0J
DnaA
3,820,000bp
ywoG ywoH
rapD
ywjG
ywkA
rho tdk rpmE
ywjI
murAB
ywjH
fbaA
ywjE
uvsE ywjC
acdA rpoE ywjF
spo0F pyrG
albA sboX sboA
ywiC
ywjA ywjB
ywiE
narI
narJ narH
narG
arfM
fnr
narK
argS
albC albB albD
albE
ywiB
albF
albG
rapF
ywhL
ywhK
ywhE
ywhB
rsfA
ywhC thrZ ywhD ywhA
speB speE
mmr
3,940,000bp
ywgA ywgB ywfO
ywzC
ywfI ywfH
ywfM ywfL ywfK pta
ywdL
ywfG
ywfF
ywfE
ywfD
ywfC ywfA ywfB
rocC
3,960,000bp
rocB
rocA
rocG
spsL spsJ spsE yweA spsK spsI spsG spsF spsD spsC spsB
spsA
ywdH
ywdJ ywdK
ywdI
ywdC
ung ywdF
ywdE ywdD
3,980,000bp
Spo0J
DnaA
3,920,000bp
ywhH phrF
thiD
ywcJ
sacA ywdA
sacP
vpr
ywcI sacT
ywcE
ywcH
nfrA
rodA
qoxC qoxD qoxB
ywcA ywcB
ywzA
qoxA
galT
galK
ywcC ywcD
ywbG ywbH ywbF
ywbN ywbO
ywbM ywbL
thiE
thiM
ywbI
ywbE
ywbD
ywbC
ywbA ywbB
epr
sacX
sacY
tyrZ ywaE
gspA ywaF
ywaD
dltA
dltB
menA ywaC
Figure S2-10
dltD dltC
dltE ywaA
katX
licH
licC licA
licR licB
yxlJ yxzF
yxlH
yxlA
yxlC yxlD yxlG yxlF yxlE sigY
yxkJ
yxkO cydD
cydC
cydB cydA
yxkI
yxzE
yxkH msmX yxkF
aldY
yxkC
yxkD
galE
yxjM yxjO yxjN
yxkA
yxjL pepT
yxjI yxjJ yxjH
yxjG
yxjF
4,040,000bp
4,060,000bp
4,080,000bp
Spo0J
DnaA
4,020,000bp
yxjA
scoB yxjC yxjF scoA
citH
yxiS yxiT katE
yxjB
hutH hutP
yxiO
bglS licT
yxiP
deaD
yxiM
yxiI yxiF yxiK yxzG yxiG wapA yxiL yxiJ yxiH yxzC yxxG
yxxF
yxiE bglH
bglP
yxxD yxxE yxiD
hutI
yxeD yxeE yxeB
hutG hutM
yxiB yxiC yxiA
pdp
nupC
dra deoR yxxB yxeR
yxeQ
yxeN yxeL yxeP yxeO yxeM yxeK
4,140,000bp
yxeI yxeJ
yxeG yxeH yxeF
iolR iolS
yxdM yxeA
yxeC
yxdL yxdK yxdJ fbaB iolI
iolH idh
4,160,000bp
iolF
iolE iolD
iolC
yxcD yxcE csbC
iolB mmsA
yxbG
htpG
yxcA
yxbA asnH yxbB yxnB
aldX
yxbC yxbD
yxbF
4,180,000bp
Spo0J
DnaA
4,120,000bp
hutU
asnH yxaM
yxaI
yxaJ
yxaH
yxaG yxaF
yyaC
yyaB
gntK gntR
yxaC
yxaD
yxaB
gntP
yxaA
gntZ
ahpF ahpC
yydK
bglA
yyzE
fbp
yydG yydJ yydI yydH yydF
yycS
yydD
yydB yydC
yydA
yycR
rapG phrG
yycP yycQ
yycN yycO
rocF rocE
yycC yycD yycB
rocR
rocD
yyxA
yycJ yycI yycH
yycG
yycF
Spo0J
DnaA
yxaL
yxnA
yyaE
yyaD
spo0J soj
yyaA
gidB gidA
thdF
spoIIIJ rpmH jag rnpA
Figure S2-11
purA
dnaC yycE
yyzB
yycA
cotF
rplIyybT
yybS
ppaC
yybR
yybO
yybP
yybM yybK yybN yybL yybJ
yybG
yybI yybH
yybE
yybF
yybD yybC
yybB
yyaS yyaT
yybA
yyaR
yyaP yyaQ
yyaN yyaO yyaM yyaL
tetB
tetL
yyaJ
yyaK
yyaH rpsR yyaF maa ccpB exoA ssbrpsF
Figure S3 10
rocA yvdC
yvdD
sda
NIS2022 + 100µM IPTG
rocD
gidA 1
thdF ykuP
yveL
ykuN ywlC dhbA
ykuO
yydA
0.1 0.1
1
10
NIS2022 – IPTG 10
sda
yvdC
yvdD
NIS2022 + 100µM IPTG
rocA
rocD yyaA gidA
1
thdF yveK ykuN
ykuP
ykuO
dhbA
yveL yydA
ywlC
0.1 0.1
1 NIS2022 – IPTG
10
