Table S1. Primers used for the construction of

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Table S1. Primers used for the construction of plasmids used in this study

a

Primer

Sequence a

Location on the genome b

dnaA.f

cgcgaattcTTATTACAGATATCACACCG c

1215...1234

dnaA.r

gcgcccgggTTTAAGCTGTTCTTTAATTTCTTTTAC

1747...1721

spo0J.f

cgcgaattcAGCGTGAAGATTTATCTCCG

4204583...4204603

spo0J.r

gcgctcgagTGATTCTCGTTCAGACAAAAG

4205088...4205069

1dnaA-HiF

cgcaagcttGATCTAGAAACGAGAATTGC

1238...1257

1dnaA-LinkR

tgcgatcctctcatttcaagTTTAAGCTGTTCTTTAATTTC

1747...1727

2dnaA-HisF

caccaccaccatcatcaccatTAGCAGGACCGGGGATCAATC

1748...1768

2dnaA-BaR

gcgggatccTCGTCAAATACTGATTTTGG

2251...2232

Linker-12His

cttgaaatgagaggatcgcatcaccatcaccatcaccaccaccatcatcaccat

Capital and lowercase letters indicate sequences derived from the B. subtilis genome and others, respectively. b Numbers refer to the SubtiList database (http://genolist.pasteur.fr/SubtiList/index.html). c Underlined sequences represent the recognition sites of restriction enzymes.

Table S2. Genes up- or downregulated by DnaA gene a

x1/5 DnaA b

x5 DnaA c

ratio

function

downregulated dnaA (dnaAN) d 0.65 0.39 0.13 replication 0.48 0.18 0.38 replication dnaN (dnaAN) d ykuN (ykuNPO) 1.58 0.73 0.46 similar to flavodoxin ykuO (ykuNPO) 1.84 0.73 0.40 unknown ykuP (ykuNPO) 2.22 0.89 0.40 similar to flavodoxin dhbA (dhbACEB) 1.04 0.59 0.57 biosynthesis of siderophore group nonribosomal peptides 0.70 0.34 0.49 unknown ywlC 0.70 0.45 0.64 unknown yydA noc (thdF/gidA/gidB/noc) 2.78 1.76 0.63 cell division gidB (thdF/gidA/gidB/noc) 3.11 1.70 0.55 translation gidA (thdF/gidA/gidB/noc) 2.10 1.16 0.55 translation thdF (thdF/gidA/gidB/noc) 1.77 1.00 0.56 translation upregulated 1.17 3.52 3.00 sporulation sda d yveL (yveKL) 0.19 0.56 2.95 similar to capsular polysaccharide biosynthesis protein yveK (yveKL) 0.24 0.65 2.71 similar to capsular polysaccharide biosynthesis protein yvdD (yvdDyvdC) 1.01 2.88 2.85 unknown yvdC (yvdDyvdC) 1.28 3.60 2.81 unknown protein with MazG nucleotide pyrophosphohydrolase domain 0.34 1.97 5.79 arginine catabolism rocA rocD (rocCDE) 0.76 2.06 2.71 arginine and proline metabolism a Genes that are downregulated (2.5) in a DnaA-dependent manner in two independent experiments are specified. The operon structure is indicated in parentheses. b Expression levels in NIS2022 cells grown in the absence of IPTG to repress the DnaA level to 1/5 times that in the wild-type strain, CRK6000. Expression level is the average of signal intensities of probes in coding sequences after trimming two of the lowest and highest intensities. c Expression level in NIS2022 cells grown in the presence of 0.1M IPTG to stimulate the DnaA level to 5 times that in the wild-type strain, CRK6000. d Expression levels of dnaA, dnaN and sda were calculated using probes in regions 168..333, 1751..1938, and 2646601..2646849, respectively.

840 210

420

630

1020 680

0

340 0

Cell Counts

1360

1050

1700

Figure S1

0

200

400

2 4

600

8

800

1000

0

200

400

2 4 origin/cell

600

800

8

1000

40,000bp

60,000bp

80,000bp

Spo0J

DnaA

20,000bp

dnaA

dnaN

recF yaaA

gyrB yaaB

gyrA

rrnO-16S

rrnO-23S

guaB rrnO-5S

dacA

yaaD yaaE serS

dck dgk yaaH

yaaC

recR bofA yaaK yaaL rrnA-16S

yaaJ dnaX

rrnA-23S

xpaC rrnA-5S csfB yaaN

holB yaaR yabB yabC tdk yaaQ yaaT yabA yazA

yaaO

yaaI

metG

yabD yabE

ispE ksgA sspF spoVG rnmV yabG veg purR yabJ gcaD

mfd ctc spoVC yabK

prs

yabM spoVT

yabN

yabQ yabP divIC yabO yabR

spoIIE

yabT yabS yacA

ftsH hprT

yacB

hslO

yacD cysK pabB

pabC pabA sul

folK yacF folB yazB lysS

rrnJ-16S rrnJ-23S

rrnW-16S rrnW-23S

abrB

140,000bp

160,000bp

180,000bp

Spo0J

DnaA

120,000bp

rrnW-23S

ctsR mcsA rrnW-5S mcsB clpC

radA

yacK

yacL

ispF gltX

cysS cysE

ispD

nusG yacO sigH secE rplK rplL yazC yacP rpmG rplA rplJ ybxB rpoB

rpsL fusA ybxF rpsG

rpoC

tuf

rplV rpmC rplX rpsH rpsE secY rplC rplW rpsC rpsQ rplE rplF rpmD ybaC rpsJ rplD rplB rpsS rplP rplN rpsN rplR rplO

adk map

infA rpsK rpsM rpoA ybxA ybaF rpsI cwlD rpmJ rplQ ybaE truA rplM ybaJ ybaK ybaL

kbaA

gerD

240,000bp

rrnI-23S

rrnH-16S rrnH-23S

rrnG-16S rrnG-23S

ybaR rrnG-5S

ybbM sigW ybbP ybbR

ybaS

feuC ybbA

ybaN

260,000bp

feuB feuA ybbB

ybbC

ybbD

ybbE

ybbF

ybbH ybbI

ybbT

ybbK ybbJ

280,000bp

Spo0J

DnaA

220,000bp

rrnI-16S

glmS

ybbU

adaB adaA ndhF

ybcC ybcD

ybcI ybcH ybcF

ybcL

ybcP ybcO ybcM

ybcT ybcS ybdAybdB

ybdE ybdK ybdD ybdG ybdJ ybdL

alkA

ybdO

ybxG

ybxH csgA

ybdMybdN

cypC

ybxI

ybfA ybeF ybfB

ybeC

ybyB

purT

ybfF ybfE ybfG

glpQ glpT

ybgB ybgA ybgE

ybfP ybfQ

ybfH ybfI

gltP

gamP

340,000bp

gamA

ycbA

ybgF

mmuM

ybgH

ycbB

ycbC ycbD

ycbE

ycbF

ycbGycbH

ycbJ

ycbK ycbM yczA ycbL ycbN ycbO

ybgJ

ycbR cwlJ phoD

ycbP

360,000bp

tatCD tatAD

ycbU

pcp

yccC

lmrB

lmrA

lip

natB natA

yccF

yczC

yccK

yccG yccH

ycdA

380,000bp

Spo0J

DnaA

320,000bp

ybfK ybfM ybfO mpr ybfJ pssA psd ybfN

ycdB

ycdA

ycdC

rapJ

ycdD

ycdFycdG

ycdH

ycdI yceA

yceD yceF yceC yceE yceG

yceB

yceH

opuAC opuAA opuAB

yceI

yceJ

yceK

amhX

ycgA

amyE ycgB

ldh

ycgF ycgE ycgG

lctP

mdr

ycgH

ycgI nadE

tmrB

aroK

ycgJ

ycgK cah

ycgL ycgM ycgN

ycgO

ycgP

ycgT

ycgR ycgQ ycgS

nasA

nasF

Figure S2-1

nasD nasE

nasC

nasB

yckD yckC yckE

yciA yciB yciC

yckB yckA

hxlR srfAA

nucA nin tlpC

hxlB hxlA

srfAB

comS

srfAC

440,000bp

460,000bp

480,000bp

Spo0J

DnaA

420,000bp

ycxA srfAD

srfAC

yclC yclB

ycxD

ycxC ycxB

yckK

sfp yczE yckI yckJ

yclDyclE

yclA

yclG

yclF

gerKA

gerKC gerKB

yczF

yclJyclK

yclH

rapC

phrC

yclI

yclN yclO yclP yclQ

yclM

yczG

ycnB

gabT

gabD

ycnE ycnC ycnD gabR

ycnJ ycnI

mtlD

ycsA

sipU

ycnK

ycsD

ycsE

ycsF ycsG

ycsI kipI kipA

kipR ycsKyczI pbpC

yczH

ycsN mtlR

yczJ

540,000bp

ydaE ydaG ydaD ydaF ydaH

ydaB

ydaC

lrpC topB

ydaJ ydaK ydaL

ydaM

ydaN

ydaO

ydaP mutT

ydzA

mntH

560,000bp

ydbB ydbA gsiB ydbC

ydaQ

ydaT ydaS

dctS

dctR dctP

ydbD dctB

580,000bp

Spo0J

DnaA

520,000bp

mtlA ycnL

glcU gdh

dctP

ydbI

ydbL ydbJ ydbK ydbM

ydbO

ydbN

ddl

murF

ydbT ydbS

ydbR

ydbP

ydcC acpS

alr

rsbR rsbW ydcG ydcE rsbT sigB ydcH ydcD rsbS rsbU rsbV rsbX ydcF ydcI

ydcO ydcQ sacV ydcP

ydcK

ydcA

ydcL

ydcR

ydcT yddD yddA yddE ydcS yddB yddC

yddG yddF

yddH

yddI yddJ

ydcM ydcN

rapI

yddM phrI

yddK

yddN

yddQ yddR yddS

yddT

ydeAcspC

ydeD

ydeC ydeB ydzE

lrpB

640,000bp

ydeF

ydeG

ydeI ydeH

ydeE

ydeL

ydeM

ydeO

ydzF ydeN

ydeJ ydeK

ydeQ

ydeP

ydfA ydeS ydeT

ydfB

ydeR

660,000bp

ydfD

ydfC

ydfF

ydfE

ydfH

ydfO ydfQ ydfN ydfP

ydfI ydfJ

ydfG

nap

ydfK ydfL ydfM

ydzH ydfR

680,000bp

Spo0J

DnaA

620,000bp

lrpA

ydfS

ydfR

ydgD ydgC ydgE

ydgB cotP ydgA

ydgH ydgG

expZ

ydgF

ydgK

ydgJ ydgI

dinB

ydhCydhD

ydhB

ydhE

ydhG

ydhI ydhH

ydhFphoB

ydhO ydhN ydhM

ydhJ ydhK

ydhP

ydhR ydhQ ydhS ydhT

ydhL

ydiC gcp rrnE-5S ydiB ydiD

rrnE-16S rrnE-23S

ydhU

ydiH tatCY moaC tatAY

ydiM

ydiN ydiO

ydiP

ydiQ

ydiR ydiS

ydjA

ydiL ydiK

ydiF

ydjBydjC

gutB

gutP

ydjE

ydjG pspA ydjH ydjI

gutR

740,000bp

ydjN ydjM

ydjK

ydjJ

ydjP ydjO yeaA cotA

ydjL

760,000bp

yeaB yeaC

yeaD yebA

guaA

pbuG

yebE purE yebC yebD yebG purK

purB

gabP

780,000bp

Spo0J

DnaA

720,000bp

groEL groES

purQ purB purC purS purL

purF

purH purM purN

purD

yecA

yezC

yerA

yerB yerC

yerD

pcrB pcrA

ligA

yerH

gatA gatC

yerI

opuE sapB

gatB

yerP

yerO

yerQ

yeeA yefC

yefA

yefB

yeeB

yeeC

yeeG

rapH

yeeI

cotJC yeeK yesE cotJB yesJ yesL yezE yesF cotJA yesK yesM

yezA yeeD yeeF

Figure S2-2

yesN

yesO

yesP yesQ yesR yesS

yesT yesV yesU yesW

yesX

yesZ yesY

yetA

lplA

lplB

lplC lplD

yetF

yetI

yetH yetG

yetJ yezD yetK yezB

yetM

yetL

yetO

yetN

yfnI

yfnH yfnG yfnF

840,000bp

860,000bp

880,000bp

Spo0J

DnaA

820,000bp

yfnF yfnE

yfnD

yfmT

yfnC

yfmP yfmQ yfmO

yfmS yfmR

yfnB yfnA

yfmN

yfmL

yfmM

pel yflT yfmA

yfmG yfmH

yfmK

yfmJ

yfmI

yflS

citS

citT yflP

citM

yflN

yflM

yfmFyfmE yfmD yfmC yfmB

yflK

yflL

yflC

yflI yflG yflJyflH

yflE

treP

yfkT

yfkR yfkS

treA

treR yfkO

yfkQ

yfkI yfkJ yfkH

yfkM

yfkN

yfkL

940,000bp

yfkK

yfkE

yfjS yfjT

yfkD

yfkA yfkC yfkB

yfkF

yfjP

yfjR

yfjO

yfjN

yfjL yfjM

acoA acoB acoC acoL

acoR

sspH

malA

yfjE yfjC yfjF yfjD yfjB

yfjQ

960,000bp

yfiA malP

yfiB

yfiC

yfiDyfiE

yfiF

yfiG

yfjA

980,000bp

Spo0J

DnaA

920,000bp

yflD yflA yflB

nagP

yfiG

yfiH

yfiI

yfiJ

yfiK yfiL

yfiM

yfiN

lipB yfiQ

yfiT

yfiS yfiR

padR

yfiU

yfiWyfiX

yfiZ

yfiV

yfhA

yfhB yfhC

yfhG yfhH yfhI

yfhF yfhE yfhD

yfiY

yfhK yfhM yfhJ yfhL csbB

yfhO

yfhQ

sspK

yfhP

fabL

sspEygaC ygaB ygaD

yfhS

ygaE

gsaB

rrnD-16S rrnD-23S

ygaJ ygzA thiC

rrnD-5S

ygzB

senS

spo0M

ygaK

1,040,000bp

ssuB

ssuA

ssuCssuD

yhzA ygaN

katA

yhbA

yhbD yhbF yhbB cspR yhbE prkA

yhbH

yhbJ yhbI yhcA

yhcE yhcC yhcF yhcH yhcB yhcD yhcG yhcI

ygaO yhzB

yhcJ

cspB

1,060,000bp

yhcL

yhcK

yhcO yhcN yhcP

yhcM

yhcR

yhcS

yhcQ

yhcV yhcX yhcU yhcW

yhxA

yhcT

1,080,000bp

Spo0J

DnaA

1,020,000bp

perR ygaF ygxA

yhxA

glpF glpP glpK

glpD

yhxB

yhcY

yhdA yhcZ yhdC

lytF yhdB

spoVR

ygxB yhdE

lytE

phoA

citA

yhdF

yhdG

yhdH

citR

yhdJ

yhdI

yhdN yhdO

yhdL yhdK sigM

yhdP

yhdR

yhdQ

yhdY yhdX yhdZ

yhdT

ccrB ccrB yhdW

yhdS

yheN

nhaC

yheH

yheB

sspB yheF yheD yheG yheE

nhaX

yheC

yheA

yhaZ

yhaX hemZ

yhaY

1,140,000bp

yhaR yhaQ yhaP

yhaU

yhaO

yhaN

yhaM yhaL

yhaT yhaS

prsA

ecsB ecsA

yhaI

yhaJ yhaG yhaK hpr yhaH serC

hit

1,160,000bp

ecsC

pbpF

yhaA

yhfA

hemE

hemH hemY

yhgE yhgD

yhfC

yhgC yhgB

fabHB

yhfE yhfF

yhfD

gltT

yhfI yhfJ

yhfK

yhfH

1,180,000bp

Spo0J

DnaA

1,120,000bp

yheI yheJ

dat

yhfL yhfK

yhfP yhfO yhfQ

yhfN

yhfM

aprE

yhxC

yhfS yhfR yhfT

hemAT yhfU yhfW

comK

yhzC yhxD

yhjA

yhjB

sipV yhjE yhjG

yhjD yhjC

yhjH

yhjM

glcP

yhjJ

yhjK yhjL

yhjP

yhjN

yhjO

addB

yhjR yhjQ

addA

sbcD yirY

yisB

yisK

yisL wprA

gerPD gerPA gerPF gerPC yisI gerPE gerPB yisJ

Figure S2-3

asnO

yisN

yisP

yizA

yisR degA

yisQ

yisS

yisV

yisU yisT

yisY

yisX

yitC yitD

yitA yisZ

yitB

yitL

yitE yitF

yitG

yitH yitI yitJ

yitK

yitQ

yitN yitP yitM yitO

yitR nprB

yitT ipi

yitS

yitV

yitU

yitW yitY

yitZ argC argJ

argB argD

carA carB

1,240,000bp

1,260,000bp

1,280,000bp

Spo0J

DnaA

1,220,000bp

carB

argF yjzC

yjaU

med fabHA yjaV comZ fabF

yjzD

yjaZ appD appF appA

appB appC yjbA

yjzB

oppA

oppB oppC oppD oppF yjbB

yjbF mecA

yjbC spxA

trpS

yjbM yjbL ppnK yjbO

yjbG

yjbE

yjbH yjbI yjbJ yjbK

yjbV fabI yjbX

yjcA

yjcB yjcC

cotZ cotX cotV cotY cotW

yjbP

yjcI

yjcD

yjcJ

yjcE yjcG yjcF yjcH

1,340,000bp

trnSL-Val2

yjcL yjcK

yjcQ yjcP yjcS yjcR fabG

yjcN yjcO

yjcM

manR

manP

manA yjdF

yjdI

yjdH yjdG

yjdB

1,360,000bp

yjeA

yjdJ ctaO

yjfC

cotT

yjhA

yjgC

yjgD

yjgA yjgB

yjfAyjfB

yjhB

yjiB

yjiC

yjlD yjlB yjlC

yjjA

yjiA

uxaC

yjkA yjkB yjlA

1,380,000bp

Spo0J

DnaA

1,320,000bp

tenI thiG tenA goxB thiS thiF

yjbQ

uxaC

yjmB

yjmC yjmD uxuA yjmF exuT

exuR uxaB

uxaA

yjoB

yjnA

rapA

yjoA

xlyB phrA

xpf xkdC xtrA xtmB xkdB xkdD xtmA

yjqByjqC

yjpA

xkdE

xkdFxkdG

xkdI xkdK xkdH xkdJ

xkdS xkdQ xkdT xkdP xkdR xkdUxkdV

xkdN xkdM xkdO

xepA xlyA xkdX xhlB xkdW xhlA

ykcA

pit spoIISA spoIISB

xre xkdA

yjqA

ykcC

ykaA ykbA

proG dppA dppB dppC dppD dppE

ykfAykfB

ykfC ykfD

htrA

1,440,000bp

ykhAhmp

ykgB

ykgA

ykjA

ykkC ykkA ykkD ykkB ykkE

proB proA

ykzH

yklA

ykzA

ykmA

1,460,000bp

ykoH ykoG

ykoB

metE guaD

ispA

ykoC ykoD

ykzD ykoI ykoJ ykoK

ykoF ykoE

ykzB ykoM ykoL

tnrA

ykoP

ykoN

1,480,000bp

Spo0J

DnaA

1,420,000bp

ykcB

ykoP

ykoS

ykoQ

ykoY ykoX

ykoT

ykoU

sigI ykrI

ykrL

ykrM

ykrK sspD

ykoV ykoW

ykzE

ykrP

kinE

ogt

ykrV

ykrS

ykrT

ykrU

ykrW

ykrY ykrX ykrZ

eag spo0E

ykvA

ykvE

kinD

ykvJ ykvL ykvK ykvM

ykvI

motA motB clpE

ykvP

ykvQ

ykvN

ykvR

ykvT ykvU

ykvV ykvW

ykvY

ykvZ glcT

ptsI ptsH

ptsG

splB splA

ykvS

mcpC

ykwC

ykwB

1,540,000bp

ykuA

kinA

ykwD

cheV

ykuF ykuG

ykuC ykyB

patA

ykuI ykuH

ykuK ykuL ykuO ykuQ ykuJ ykzF ccpC ykuN ykuP ykuR ykuS

ykuE ykuD

1,560,000bp

ykuT

ykuV ykuU rok

moeB moaE mobA moeA mobB moaD

yknT

1,580,000bp

Spo0J

DnaA

1,520,000bp

ykvO

yknU

yknV

yknX yknW yknYyknZ

fruR fruK fruA

sipT

ykpA

ykoA

kinC abh

ykpB

ampS

mreBH ykpC

ykqA ykqB adeC

ykrA

ykqC

ykzG

ykyA pdhA pdhB pdhC

def

pdhD

yktA

slp

speA

yktB

yktC ykzI ykzC

yktD

ylaA

nprE

ylaC ylaB ylaD

ylaG

ylaE ylaF

ylaH

ylaJ ylaI

ylaK

ftsW ylaM ylaN

ylaL

Figure S2-4

pycA

ctaB

ctaA

ctaC ctaD

ctaF ctaE ctaG

ylbC ylbB

ylbA

ylbF ylbE ylbH ylbD ylbG ylbI

ylbJ

rpmF ylbN ylbO

ylbK ylbL

ylbM

ylbQ yllA

ylbP

yllB ftsL mraW pbpB

spoVD

murE

mraY murD

ylxW ftsA spoVE murG murB divIB ylxX sbp

ftsZ

bpr

1,640,000bp

1,660,000bp

1,680,000bp

Spo0J

DnaA

1,620,000bp

sigE spoIIGA sigG ylmA ylmB

bpr

ylmF ylmD ylmG divIVA ylmC ylmE ylmH ileS

lspA ylyA ylyB

pyrP pyrR

pyrB pyrC

pyrAA carB

pyrK pyrD pyrFpyrE cysHcysP

sat

ylnD cysC ylnE ylnF

yloB

yloC

ylzA yloI gmkrpoZ

priA

fmt def

yloM

yloN prpCprkC

yloQ rpe

yloS

yloA

recG sdaAA sdaAB

plsX ylpC

rnc fabD fabGacpP smc

ftsY

rpmB

1,740,000bp

ylqD rpsP trmD ylqC rimM rplSylqF rnhB ylqG

ffh ylxM

sucC ylqH sucD smf

topA

gid

clpY codV clpQ

flgC fliF codY flgB fliE

fliG

fliH fliI

fliJ

ylxF

fliK

flgE ylxG

ylqB

1,760,000bp

1,780,000bp

Spo0J

DnaA

1,720,000bp

yloU yloV spoVM

flgE fliLfliM

fliY

fliZ fliR cheY fliP fliQ flhB

flhA

flhF

ylxH cheB cheA

cheC sigD rpsB cheW cheD ylxL tsf

frr pyrH

cdsA uppS dxr

yluC

proS

nusA ylxS

polC

ylxR infB ylxQ

rbfA ylxP truB ribC

pnpA rpsO

ylxY mlpA

spoVFA asd ymxH spoVFB

dapG

dapA ymfA

tepA spoIIIE

ymfC

ymfD ymfF ymfH ymfE ymfG

fabG

ymfL ymfJ ymfM cinA ymfK pgsA

recA

pbpX

ymdA

tdh ymdB spoVS

kbl

ymcB

cotE ymcA mutS

mutL

pksApksB pksC

pksF acpK

pksD pksE

pksG

pksI pksH pksJ

ymcC ymzD

1,840,000bp

1,860,000bp

1,880,000bp

Spo0J

DnaA

1,820,000bp

pksL

pksM

pksN

pksR

ymaCymaD

pksS

1,940,000bp

ebrA ebrB ymaG

ymaH ymaA ymzC nrdE ymzA

nrdF ymaB

spoVK ynbA ynbB

glnA glnR

ynxB ynzG ynaC ynaE ynaG ynzF ynaB ynaD ynaF ynaI

cwlC

xynP

xynB

xylA

xylR

1,960,000bp

xylB

yncF yncE

yncC

yncB

yncD

1,980,000bp

Spo0J

DnaA

1,920,000bp

ymaE ymzB aprX

ymaF miaA

yndD yndA ynzB

thyA

ynzH

yncM

cotC yndB tatAC

yndE yndF

yndH yndG yndJ

yndL yndK

yneB tkt yneA ynzC

yndN

yndM

lexA

yneF yneI yneE ccdA yneJ

ynzD

citB

sspP cotM yneK sspO

sspN tlp yneQ yneP

yneN

yneTparE

yneS yneR

parC

alsT

ynfC

bglC

yngB yngA yngC

ynfE

ynfF

xynD

yngD

yngE

yngFyngG yngH

Figure S2-5

yngI

yngJ

yngK ynzE

ppsE yngL

ppsD

ppsC

ppsB

ppsA

dacC

yoxA

2,040,000bp

2,060,000bp

2,080,000bp

Spo0J

DnaA

2,020,000bp

yoeB

ggt

yogA

yoeD yoeC

yoxA yoeA

yofA

yoxB yoxC yoaA

gltC

gltB

gltA

proJ

proH rtp fabG

yoaE

yoaB

yoaC

yoaF

yoaD

yozQ

yoaG

yoaI

yoaH

pelB

yoaJ yoaK

yozG yoaS yoaT

yoaQ

yoaN

yoaP yoaO

yoaR yozF

yoaV

penP

yoaZ yoaW

yoaU

yobE yobD yozI

yobB

yobA

pps

xynA

yozH

2,140,000bp

rapK phrK

yobF

yozM

yobN

yozK yobH yozL yobI

yozJ

yobL yobJ yobK

yobO

yobQ yobS yobU csaA yobR yobT yobV

yobM

2,160,000bp

yocD yocC des

yobW yocA

yozA

yocN yozN

yocF yocG

yozB yocB

dhaS

yocK yocM acpD yocL yozO

yocH yocI

2,180,000bp

Spo0J

DnaA

2,120,000bp

yoaM

dhaS

sqhC

sodF

yocS

yocR

yojI

odhB

odhA

yojO

yojN

yojM yojL

yojK

yojH

yozR

yojF yojG yojE

yojJ

yodA

yojB yojA yojC

yodC

yodB

yodI yodH

yodF

yodD yodE

cgeB cgeA

yodM yodN kamA yodJ deoD yodL yozD yozE

ctpA

yodS yodR yodT

cgeD cgeE

cgeC

phy

yotL

sspC

yotE yotH yotD yotJ yotC yotM yotIyotG yotB yotN yotK yotF

yosU

yosX yosP yosW yosS yosQ yosZ yosV yosT yosR

2,240,000bp

ligB yoqW

yosA

yosJ yosF yorY yosM yosI yosE yorX mtbP yosL yosH yosD yosB yorV yosK yosG yosC yorZyorW

yosN

yorP yorS yorO yorR yorN yorL yorT yorQ yorM

2,260,000bp

yorK

yorJ

yorI

yorG yorE yorC yorA yorH yorF yorD yorB

yoqZ

yoqY yoqX

yoqT

yoqO yoqM

yoqH yoqE yoqSyoqP yoqL yoqJ yoqG yoqD yoqU yoqR yoqN yoqK yoqI yoqF

2,280,000bp

Spo0J

DnaA

2,220,000bp

yodPyodQ

yodU

yonO yonP

yopS

yopZ yopV yopY yopT yoqA yopX yopU yoqB yopW yopR yopQ

yopM yopN yopK yopP yopO yopL

yopH yopE yopI yopG yopC yopJ yopF yopD

yopA yopB

yonV yonX

yonN

yonJ yonK

yonT yonR yonU yonS

yonG yonH yonF

yonE

yonD

yomX yonB yomZ yomW yonC yonA yomY yomVyomU

yomR yomO yomS yomP yomM yomT yomQ yomN

yomJ yomK yomI

yomG yomH

yomE yomF

yomD blyA

yomL yozP

yonI

yolJ bdbB

2,340,000bp

yolC

sunT bdbA

yolF sunA uvrX

yokF yokEyokD

yolA yolD yolB

yokK yokI yokL yokJ

2,360,000bp

yokA

yokB yokC

yokH yokG

ypoP

msrA yppQ ypnP

yplP yplQ

ypmS ypmQ ilvA ypmTypmR ypmP

ypkP thyB ypjQ ilvD dfrA ypjP ypiP yphP

2,380,000bp

Spo0J

DnaA

2,320,000bp

bhlB bhlA

metA ugtP

ilvD

ypgR

ypgQ bsaA

ypzA cspD

ypdQ ypdP sspL

ypeQ degR ypeP

ypcP

ponA ypqA yppF sspM recU

kduI kduD

ypbR ypbS

bcsA ypbQ

pbuX

xpt

ypwA

kdgK kdgT kdgA kdgR

ypvA

ypsC yptA

ypsA yprB ypsB cotD

yprA

yppE yppC ypqE yppG yppD

ypjC

nth asnS ypoC dnaD

aspB

ypmB dinG ypmA

panC panD panB birA cca

Figure S2-6

ypjH

ypjG dapB mgsA ypjD

ypjB

ypjA

qcrC

qcrB ypiB qcrAypiF ypiA

aroE

tyrA

hisC trpA trpB

trpF trpC trpD trpE

aroB aroH

aroF

cheR ndk hepT

menH mtrB hbs hepS folE spoIVA

yphF

yphE gpsA yphC

seaA

yphA ypgA ypfD

ypfB cmk ypfAypeB

sleB

ypdC

2,440,000bp

2,460,000bp

2,480,000bp

Spo0J

DnaA

2,420,000bp

fer

ypbD ypbH ypbE ypbG ypbF recQ

ypdA ypdC gudB

serA

ypzE ypaA

ypbB

ypuF

aroD

rsiX

sigX resE

resD

resC

resB

rluB spmA resA spmB dacB

ypuH ypuI scpA

ypzC

ribA ribTribH

ribD ribE

ypuD sipS

ypuE

ypzD ypuA

ypuB ypuC

ppiB

ansR

lysA

drm punA

xerD

fur

spoIIM mleA yqkK mleN

ansB

ansA

yqxK

2,540,000bp

nudF

yqjV yqzH

yqkD

yqkE

yqjU

yqkA yqkB yqjY yqjW yqkC yqjZ yqjX

proI

coaA yqjT dsdA

yqjP yqjQ

yqjM yqjL

yqjN

yqjK

2,560,000bp

yqjG yqzJ yqjF

zwf

yqjI

yqjH

bmrU bmr

yqjE

yqjD

bmrR

yqjB yqiY yqjC yqjA yqiZ yqiX yqiW

bkdB

bkdAA bkdAB lpdV

2,580,000bp

Spo0J

DnaA

2,520,000bp

spoVAA dacF spoVAD spoVAB spoIIAA spoVAF spoVAE spoVAC sigF spoIIAB

yqkF

yqzF

lpdV

buk

bcd

ptb

bkdR

yqiG

yqiQ prpD

mmgA mmgD mmgC mmgB yqiK yqiI yqiH

yqhQ yqhP

spo0A spoIVB recN

yqxC ahrC dxs

yqiD

yqiC yqiB

yqhY folD nusB accC

spoIIIAF spoIIIAD spoIIIAA spoIIIAH spoIIIAE spoIIIAB efp yqhR accB spoIIIAG spoIIIAC yqhV yqhT yqhS

yqhM yqhO mntR

gcvPB

sinI yqhG sinR

yqhH

yqzG

comGD comGG comGB comGFcomGC comGA yqzE comGE yqxL

yqxM tasA sipW

gcvPA gcvT

yqhB

2,640,000bp

yqzD yqzC

yqgY yqgW

yqhA yqgZ yqgX

yqgV yqgU yqgT

yqgS

glcK

yqgQ yqgP

yqgO rpmGA yqgN yqgM yqgL

2,660,000bp

yqfY yqfZ

yqgB

pstBA pstBB pstA pstC pstS pbpA

yqgE

sodA yqgC

yqgA

yqfW

yqfT

yqfX zur yqfU

yqfQ yqfP

yqfS yqfR

yqfO

sigA yqfN cccA

2,680,000bp

Spo0J

DnaA

2,620,000bp

yqhL

antE

sigA

yqeW

yqfL glyS yqxD yqzB

dnaG

glyQ

recO era

dgkA yqfF cdd yqfG

phoH yqfD

yqfB yqfC yqfA yqeZ

rpsU yqeY

rpsT

yqeV

yqeT yqeU dnaJ

dnaK

hrcA grpE

hemN lepA

spoIIP yqxA gpr

yqeN

comER

comEC

nadD yqeL yqeI yqeG yqeM yqeK aroE yqeH

yqeD

yqeF cwlH

yqeB

yqeC

yqzI rapE phrE yqcG

spoIVCB

nucB spoIVCA

yqcF

yqcD yqcB yqbS yqxI yqxG yqcC yqbT yqbQ yqbO yqxJ cwlA yqxH yqcE yqcA yqbR yqbP

arsB arsR arsC yqcK yqcI

2,740,000bp

yqdB

yqbN

2,760,000bp

yqaP

yqbH yqbE yqbL yqbI yqbG yqbC yqbM yqbK yqbJ yqbF yqbD yqbB yqbA

yqaT

yqaR yqaS yqaQ

yqaE

yqaN yqaL yqaO yqaM yqaK yqaJ

yqaI yqaF yqaC yqaG yqaH yqdA yqaD

2,780,000bp

Spo0J

DnaA

2,720,000bp

comEB comEA

sda

spoIIIC yqaB

yrkO

yrkR yrkQ

yrkS

yrkP

yrkM yrkN

yrkL yrkK

blt

yrkJ

yrkI yrkF yrkD yrkB yrkH yrkG yrkE yrkC bltR

bltD

yrkA

yrdQ

yrdR

gltR

trkA

czcD

yrdN

yrpB

brnQ yrdK

azlC cypA azlD azlB yrdF

yrdC yrdD yrdB

aadK yrdA

yrpC

yrpD

yrpG

yrpE sigZ

yraK yraL

yraM

yraO

yraN

csn

yraF yraD yraG adhB yraE

yraI yraJ yraH

Figure S2-7

yraB

adhA yraA

sacC

aapA

levD levG levF levE levR

yrhO

yrhP

sigV yrhM yrhL

yrhK

yrhJ

yrhE

yrhG yrhI yrhH yrzI yrhF

yrhD

yrhB yrhC

yrzA

yrhA mtn yrrT

yrrS yrrR

udk greA yrrO

yrrN yrrM yrrL

yrzB yrzL yrrK alaS

2,840,000bp

2,860,000bp

2,880,000bp

Spo0J

DnaA

2,820,000bp

glnQ glnH

alaS

glnM glnP

yrvN

yrrC yrrD

yrrI

yrrB trmU

yrvO

yrzC

yrvJ

yrvM

aspS

hisS

yrzK

spoVB

relA yrvI

apt

yrvE

yrvC yrvD secDF

yrzD

yrzG yrbD yrzF yrzH

yrzE

yrbG

tgt yajC

csbX ruvB ruvA bofC

queA

yrbE

yrbC

coxA safA

nadA

yrxA

nadC nadB

ysnE ysnD ysnF

ysxD

pheA

pheB obg

rpmA ysxB spoIVFB minD mreD radC spoIIB folC rplU spoIVFA minC mreCmreB maf comC

spo0B

2,940,000bp

valS

ysxE spoVID

hemL

hemB

hemD hemX hemC hemA

2,960,000bp

lonA ysxC

lonB

clpX

tig

ysoA

leuD leuC

leuB

leuA

ilvC

ilvB ilvH

ysnB ysnA

2,980,000bp

Spo0J

DnaA

2,920,000bp

nifS

yslB

rph ysnA gerM

gerE sdhB racE ysmB ysmA sdhA

sdhC

ysfB sspI

rnhC

lysC

uvrC ask

xsa trxA

mutSB yshE

etfA etfB ysiB ysiA lcfA

yshA yshB

yshC

pheT

pheS

ysfC

ysfD

yscB yscA

ysdB

cstA ysfE

ysgA

araQ

araP

araN

araM araL

araD araB

araA

abnA ysdC

rpmI rplT ysdA infC

lytT ysbA ysbB lytS

3,040,000bp

ytbD

dnaI ytxC ytxB dnaB

thrS

gapB ytcG speD

ytbE

ytvI

ytaF polA ytaG mutM

ytcD

phoR

phoP mdh icd

3,060,000bp

citZ

ytrI

pyk ytzA

ytwI

pfkA

accA accD ytsJ

dnaE

ytpI

ytqI

ytoI

ytnM ytnL

3,080,000bp

Spo0J

DnaA

3,020,000bp

abfA

ysaA

ytlI

ytnL

ribR

ytnJ

ytmO ytmM ytmK ytmI ytnI ytmN ytmL ytmJ

ppnK

argH ytkL fabG ytzD

argG

ackA moaB

ytxK

ytfI sppA tpx ytfJ yteJ

braB

ytcJ

ytcI

ytbJ sspA

nifZ

hisJ

ezrA

ytsP

yttP

acuB acuA acuC

rpsD

ytrP

tyrS

ytxEytxD ccpA

aroA

ytxH ytxJ ytxGmurC

ytpT

ytpS

ytzB

ytpR ytpP ytpQ ytoP

3,140,000bp

ytzH

malS

ytkP

ytnP ytmQ ytmP amyX

ytlR

ytlQ ytlP

ytiP

ytjP

ytzE

ythQ

ythP

ytfP

ytzG ytzF ytgP

3,160,000bp

opuD

ytdP

yteU yteV yteT

yteS yteR

yteP yteQ

msmR

ytcQ

ytcP

ytbQ bioI

bioB

bioD bioF

bioA

msmE

amyD

amyC melA

bioW ytaP

3,180,000bp

Spo0J

DnaA

3,120,000bp

acsA

ytoQ

melA

ytvA

leuS ytwF

ytvB

yttA

yttB

ytqA ytqB

ytsD

ytsC ytsB ytsA ytrF

ytrE ytrD ytrC ytrB ytrA ytzC

ytpB ytpA

ytoA

ytnA

ytlC ytlA ytlB ytlD

pckA

asnB

metK

ytmA ytmB

ytjA

ythA

ytkC ytkA dps luxS ytiB ytiA

ytkD

ythB ythC

mntD mntC

yteA

mntA mntB menC menE

ytxM menB menD

menF

Figure S2-8

ytdA

ytcA

ytcB ytcC

ytaA

cotS ytxO cotSA

ytaB

glgP

yuaJ

glgA

glgD glgC

glgB

rrnB-23S

rrnB-16S

yuaG yuaI

yuaE

yuaF

gbsB yuaD gbsA

yuaB yubG yuaC yuaA

yubC

yubE yubF yubD

uppP yubA

yulF

yulE

yulC yulD

3,240,000bp

3,260,000bp

3,280,000bp

Spo0J

DnaA

3,220,000bp

tgl

yulC

yulB yuxG

tlpB

mcpA

tlpA

yugO

yugT yugU

mcpB

yugS

yuzA

yugM yugN pgi

yugP

yugK

yugJ

yugIalaT

yugF

alaR

patB

kinB

kapB

yugE

yuxJ

pbpD

kapD

yuxK

yufL

yufM

yufN yufO

yufP yufQ

maeN

yufK

mrpD mrpC mrpB

mrpA

mrpG mrpF mrpE

comA yuxO comP

yufS

yuzE

comQ comX degQ yuxH

3,340,000bp

yueK

yueG yueI yueF yueJ yueH

yukF

yueE yueC yuzF yueD yueB

yukA

yukB yukC

ald

yukE yukD

yukJ yuxI

yuiG

dhbF

3,360,000bp

dhbB dhbE

dhbC

dhbA yuiI

yuiH

yuiD

yuiF

pepA

yuiC

yuiA yuiB yumB

3,380,000bp

Spo0J

DnaA

3,320,000bp

yuzC

yumC

yumB

guaC

yutJ

paiA dapF paiB yutM yutK

yuzG

yuzB

yutG

yutI

yuxL yuzD

thrB thrC hom

yutH

yutC

yutF

yunA

yutE yutD lipA

pucR

yunC yunB yunD

pucJ

pucK

pucL

pucM

yunF pucH yunE yunG

yurI

pucD pucE

pucA pucC pucB

yurH

yurJ

yurL yurM yurN yurO

yurP

yurR yurQ

yurT yurU

csd yurV

3,440,000bp

yurX

yurY

yusG yusE gcvH yusJ yusDyusF yusI

yusA yusC yurZ yusB

yusK

yusL

yusT

yusR yusQ yusS

yusM

3,460,000bp

yusZ mrgA

yusV yusX yusU yusW

yusY

cssS cssR

yuxN

yvtA

gerAA

gerAB gerAC

yvqE yvqC

fumC

yvqG yvqI yvqF yvqH yvqJ

yvqK

3,480,000bp

Spo0J

DnaA

3,420,000bp

yurG

yusO yusN yusP

yurS sspG

yurK

yvrD

yvrA yvqK

yvrB

yvrC

oxdC yvrL

yvrE yvrG

yvrH yvrI

yvgJ yvsG

fhuD

yvrN

yvrP yvrO

fhuC fhuG fhuB

yvsH

sspJ

yvgL yvgM

yvgK

yvgN

yvgO

yvgP

yvaO yvaQ yvaM yvaN yvaP

yvaA

yvgQ

yvgR

bdbD yvgTbdbC yvgW

yvgS

yvaE yvaF yvaD yvaG

acpDyvaC

rnr smpB

yvaX yvaW yvaY

opuBC opuBA opuBD opuBB

yvaK secG yvzC

3,540,000bp

yvbA opuCC opuCA yvaZ opuCD opuCB

yvbF

yvbI yvbH

yvbG

yvbK

yvbJ

eno

araR

pgm

3,560,000bp

tpiA pgk

gapA cggR

araE

yvbV

yvbT

yvbU

rsbQrsbP

yvbW

yvbX yvbYyvfW

yvfV yvfUyvfT

yvfS yvfR

3,580,000bp

Spo0J

DnaA

3,520,000bp

yvgZ yvgY

yvgX

yvaV

rsbP

yvfH

yvfO

lacA

yvfM yvfL

yvfK

lacR

yvfI

yvfG

sigL

yvfF yvfE

slr pnbA

yvfB yvfDyvfC

yveT yvfA yveS yveR yveQ yveP

yveN yveO yveM

yveLyveK

sacB

racX yveG padC yveF pbpE

yveB

yveA

yvdS yvdT yvdR

yvdP yvdQ

yvdO

clpP

yvdC

malL pgcM

yvdK

yvdJ yvdI yvdH

Figure S2-9

yvdG

yvdF

yvdE

yvdD yvdB

yvcT

yvdA

yvpB yvpA

yvcS

crh yvcR yvcQ yvcP yvcN yvcL yvcK yvcJ yvcI trxB

yvcE

yvcD

yvcC

yvcB yvzA

yvcA hisI hisF hisA

hisH hisD hisB

hisZ hisG

hprP yvoF yvoD lgt

nagA

hprK

nagB yvoA

yvnB

3,640,000bp

3,660,000bp

3,680,000bp

Spo0J

DnaA

3,620,000bp

yvmA yvmB

yvnA

yvnB

cypX

yvkN

yvlB yvlC yvlD

yvmC

uvrA yvlA yvzB

uvrB

yvhJ yvyE

yvkA yvkB

yvkC

csbA

yvjD

yvzD

yvjB

ftsX ftsE

yvjA cccB prfB

secA

fliS fliT fliD

yvyD

hag yvyC

yviF yviE csrA flgL flgK

degS yviA degU

tagO

tuaH

tuaG

tuaF tuaE

tuaD

tuaC

tuaB

tuaA lytC

3,740,000bp

lytB

lytA

gtaB

yvyH

tagA tagB

ggaB

ggaA

tagH

tagG tagF

tagE

3,760,000bp

tagC

tagD

gerBA

lytD

gerBB gerBC

pmi

ywtF ywtE

ywtG

ywtD

ywtC ywtB

ywtA ywsC

3,780,000bp

Spo0J

DnaA

3,720,000bp

comFC flgM comFB yvyG yvyF comFA

lytR

rbsA rbsR rbsK rbsD

ywsA rbsC rbsB ywsB

alsR

ywrK

alsD ywrO alsS

cotG ywrFywrE

cotB ywrJ

cotH

ywrB ywrC ywrA

ywqM

ywqN ywqO

ywrD

ywqB

ywqK ywqL ywqJ

ywqH ywqI ywqG ywqF

ywqE

ywqA

ywpF

ywqD ywqC

ywpD

ywpG ywpJ glcR ywpH ywpE

ywpB mscL

spoIIID usd

flhP flhO mbl

nrgA

ywoF

ywoE

ywoD

ywoA nrgB

ywoB ywoC

ywnG ywnH

spoIIQ ywnJ

3,840,000bp

ywnE

ywnF

ywnC

mta

ywmA

ureC ywnA ywnB

ureA ureB

ywmF csbD rapB

ywmD ywzB atpD moaA fdhD ywmE ywmC spoIID murAA ywmB atpC

3,860,000bp

ywkC ywkD

ywlA

atpG atpA

atpF atpB atpH atpE atpI upp glyA

ywlF ywlD ywlG ywlE ywlC

spoIIR ywkE ywlB ywkF prfA

ywkB ywkA

3,880,000bp

Spo0J

DnaA

3,820,000bp

ywoG ywoH

rapD

ywjG

ywkA

rho tdk rpmE

ywjI

murAB

ywjH

fbaA

ywjE

uvsE ywjC

acdA rpoE ywjF

spo0F pyrG

albA sboX sboA

ywiC

ywjA ywjB

ywiE

narI

narJ narH

narG

arfM

fnr

narK

argS

albC albB albD

albE

ywiB

albF

albG

rapF

ywhL

ywhK

ywhE

ywhB

rsfA

ywhC thrZ ywhD ywhA

speB speE

mmr

3,940,000bp

ywgA ywgB ywfO

ywzC

ywfI ywfH

ywfM ywfL ywfK pta

ywdL

ywfG

ywfF

ywfE

ywfD

ywfC ywfA ywfB

rocC

3,960,000bp

rocB

rocA

rocG

spsL spsJ spsE yweA spsK spsI spsG spsF spsD spsC spsB

spsA

ywdH

ywdJ ywdK

ywdI

ywdC

ung ywdF

ywdE ywdD

3,980,000bp

Spo0J

DnaA

3,920,000bp

ywhH phrF

thiD

ywcJ

sacA ywdA

sacP

vpr

ywcI sacT

ywcE

ywcH

nfrA

rodA

qoxC qoxD qoxB

ywcA ywcB

ywzA

qoxA

galT

galK

ywcC ywcD

ywbG ywbH ywbF

ywbN ywbO

ywbM ywbL

thiE

thiM

ywbI

ywbE

ywbD

ywbC

ywbA ywbB

epr

sacX

sacY

tyrZ ywaE

gspA ywaF

ywaD

dltA

dltB

menA ywaC

Figure S2-10

dltD dltC

dltE ywaA

katX

licH

licC licA

licR licB

yxlJ yxzF

yxlH

yxlA

yxlC yxlD yxlG yxlF yxlE sigY

yxkJ

yxkO cydD

cydC

cydB cydA

yxkI

yxzE

yxkH msmX yxkF

aldY

yxkC

yxkD

galE

yxjM yxjO yxjN

yxkA

yxjL pepT

yxjI yxjJ yxjH

yxjG

yxjF

4,040,000bp

4,060,000bp

4,080,000bp

Spo0J

DnaA

4,020,000bp

yxjA

scoB yxjC yxjF scoA

citH

yxiS yxiT katE

yxjB

hutH hutP

yxiO

bglS licT

yxiP

deaD

yxiM

yxiI yxiF yxiK yxzG yxiG wapA yxiL yxiJ yxiH yxzC yxxG

yxxF

yxiE bglH

bglP

yxxD yxxE yxiD

hutI

yxeD yxeE yxeB

hutG hutM

yxiB yxiC yxiA

pdp

nupC

dra deoR yxxB yxeR

yxeQ

yxeN yxeL yxeP yxeO yxeM yxeK

4,140,000bp

yxeI yxeJ

yxeG yxeH yxeF

iolR iolS

yxdM yxeA

yxeC

yxdL yxdK yxdJ fbaB iolI

iolH idh

4,160,000bp

iolF

iolE iolD

iolC

yxcD yxcE csbC

iolB mmsA

yxbG

htpG

yxcA

yxbA asnH yxbB yxnB

aldX

yxbC yxbD

yxbF

4,180,000bp

Spo0J

DnaA

4,120,000bp

hutU

asnH yxaM

yxaI

yxaJ

yxaH

yxaG yxaF

yyaC

yyaB

gntK gntR

yxaC

yxaD

yxaB

gntP

yxaA

gntZ

ahpF ahpC

yydK

bglA

yyzE

fbp

yydG yydJ yydI yydH yydF

yycS

yydD

yydB yydC

yydA

yycR

rapG phrG

yycP yycQ

yycN yycO

rocF rocE

yycC yycD yycB

rocR

rocD

yyxA

yycJ yycI yycH

yycG

yycF

Spo0J

DnaA

yxaL

yxnA

yyaE

yyaD

spo0J soj

yyaA

gidB gidA

thdF

spoIIIJ rpmH jag rnpA

Figure S2-11

purA

dnaC yycE

yyzB

yycA

cotF

rplIyybT

yybS

ppaC

yybR

yybO

yybP

yybM yybK yybN yybL yybJ

yybG

yybI yybH

yybE

yybF

yybD yybC

yybB

yyaS yyaT

yybA

yyaR

yyaP yyaQ

yyaN yyaO yyaM yyaL

tetB

tetL

yyaJ

yyaK

yyaH rpsR yyaF maa ccpB exoA ssbrpsF

Figure S3 10

rocA yvdC

yvdD

sda

NIS2022 + 100µM IPTG

rocD

gidA 1

thdF ykuP

yveL

ykuN ywlC dhbA

ykuO

yydA

0.1 0.1

1

10

NIS2022 – IPTG 10

sda

yvdC

yvdD

NIS2022 + 100µM IPTG

rocA

rocD yyaA gidA

1

thdF yveK ykuN

ykuP

ykuO

dhbA

yveL yydA

ywlC

0.1 0.1

1 NIS2022 – IPTG

10