The influence of high concentration of monosaccharides on development and physiology in house cricket, Acheta domesticus. House cricket as a model in diabetes research M. Potrzebska1, K. Berger2, M. Olszanecka2, L. Ząbek2, K. Boraczewska2, M. Ludwik2, B. Kochańska3, J. Francikowski1, M. Krzyżowski1 1Department
of Animal Physiology and Ecotoxicology University of Silesia in Katowice, Bankowa 9, 40-007 Katowice 2Academy of Young Biologists, Youth Palace in Katowice 3Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences email:
[email protected]
Introduction The fruit fly, Drosophila melanogaster is a model organism in the studies of the origins of many human diseases, including diabetes. In the area of these studies, white-eye gene mutants are used, which allows testing an association of tryptophan with type 2 diabetes. Nevertheless, the fruit fly is an insect with holometabolous development, and the body size can often be a limitation in conducting many laboratory measurements. Usage of the new line of whiteeyed house cricket (Acheta domesticus) mutants (Fig.1) creates new possibilities, because of its hemimetabolous development which is much more similar to mammalian. This allows to create a better simulation of the processes of diabetes development in a growing insect. Results of the studies with the use of various concentrations of glucose and fructose in food confirmed that their high doses cause changes in the physiology (Fig. 2) and development (Fig. 3) of crickets, similar as in case of the fruit fly.
B Fig.1. Photos of eyes of black (A) and white (B) stain Acheta domesticus, (scale bar A = 88mm, B = 82 mm.)
Materials and methods 10 days larvae 50 insects per container 3 container for each group
30 day Body length Larvae mortality
Food with additional sugar F= 20% Fructose G= 20% Glucose
40 day Body length Larvae mortality
Counting imago crickets
Results 16
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Fig.3. Changes in maturing success for black and white strain males and females of A. domesticus treated with glucose and fructose (20%). Cox regression analysis.
Fig.2. Changes in size (A) and mortality (B) in 30 and 40 days of larvae for black and white strain males of A. domesticus treated with glucose and fructose (20%). Tukey’s multiple comparisons test.
Conclusions No differences in body length in larvae was observed during development on food containing tested sugars No significant mortality of larvae during development on food containing tested sugars Increased mortality of larvae treated with sugars just before the transformation The research was founded by Grant of Rector of University of Silesia in Katowice
Males tended to show lower maturing success White eye crickets matured in a significantly lower proportion than black ones Treatment with sugars caused significant decrease in maturing success Effect of fructose appeared to be significantly milder in white eye crickets In males the effect of fructose showed marginally weaker effect on maturation success Background uploaded from:sweetapolita.myshopify.com
