1
Article
4
The Organization of Mitochondrial Supercomplexes is Modulated by Oxidative Stress in vivo in Mouse Models of Mitochondrial Encephalopathy
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Mir R. Anwar †, Amy Saldana-Caboverde †, Sofia Garcia and Francisca Diaz *
2 3
6 7 8 9
Department of Neurology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA. * Correspondence:
[email protected] † Those authors contribute equally to this study.
Supplementary Materials
A
B
Hippocampus Cox10 60
UQCRC1 % of Total
UQCRC1 % of Total
Ctrl KO 40
20
0
C
High
CI+CIII
UQCRC1 % of Total
UQCRC1 % of Total
* **
CIII
Cingulate Cortex RISP
**
40
**
20
0
High
CI+CIII
High
CIII
F
Piriform Cortex COX10 Ctrl KO
60 40 20
CI+CIII
CIII
Piriform Cortex RISP KO 100
*
80
UQCRC1 % of Total
UQCRC1 % of Total
CI+CIII
Ctrl KO
80
10 11 12 13 14 15 16 17
High
Ctrl KO
40
0
** 20
60
60
E
Ctrl KO
40
D
Cingulate Cortex COX10
0
**
0
CIII
80
20
Hippocampus RISP 60
Ctrl KO
60
*
40 20
High
CI+CIII
CIII
0
High
CI+CIII
CIII
Fig S1. Quantification of UQCRC1 signal in blue native gels of mitochondria from hippocampus, cingulate cortex and piriform cortex of COX10 and RISP KO mice. Mitochondria were extracted for BN-PAGE from Ctrl and KO mice from: A) and B) Hippocampus form COX10 and RISP KO respectively; C) and D) Cingulate cortex from COX10 and RISP KO respectively; E) and F) Piriform Fig S1 cortex from COX10 and RISP KO. Signals obtained using the UQCRC1 antibody shown in Fig1 were quantified by densitometry using Image J and expressed as % of total signal. Bars represent mean and standard deviation. (*) p
