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S162

Asian Pac J Trop Med 2014; 7(Suppl 1): S162-S166

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Asian Pacific Journal of Tropical Medicine journal homepage:www.elsevier.com/locate/apjtm

Document heading

The

doi: 10.1016/S1995-7645(14)60224-8

relationship between progesterone and biochemical constituents of amniotic fluid with placenta traits in Iranian crossbred ewes (ArkharMerino伊Ghezel) 1

1

2*

Ali Olfati , Gholamali Moghaddam , Nasroallah Moradi Kor

, Mitra Bakhtiari3

Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran

1

Department of Reproduction Physiologies, Iranian Society of Physiology and Pharmacology, Tehran, Iran

2

Department of Anatomical Sciences, Faculty of Medicine, University of Medical Sciences, Kermanshah, Iran

3

ARTICLE INFO

ABSTRACT

Article history: Received 27 May 2014 Received in revised form 28 Jun 2014 Accepted 15 Jul 2014 Available online 20 Aug 2014

Objective: To determine the values of amniotic fluid (AF) progesterone and biochemical constituents and its associated placenta traits in Iranian crossbred ewes (Arkhar-Merino×Ghezel). Methods: Sixty ewes (2-5 years old, weighing 40-50 kg) were treated with controlled internal drug release induced 30 mg progesterone for 14 d and were injected with 400 IU pregnant mare serum gonadotropin at the time of CIDR withdrawal. After the detection of estrus, ewes were hand-mated. After expelling of each fetus accompanied by fetal membranes, 10 mL AF was taken from fetal sac for spectrophotometer methods (glucose, urea, creatinine, total protein, cholesterol, triglycerides, calcium and phosphorus) and radioimmunoassay (progesterone) analysis. Results: Results indicated that there were highly positive correlations between placental efficiency and cotyledon density (r=0.764, P