Carpenter, SR, Carago, NF, Correll, DL, Howarth, RW, Sharpley, AN, Smith,. VH. 1998. Nonpoint pollution of surface waters with phosphorus and nitrogen.
University of Nevada, Reno
Bioavailability of dissolved organic nitrogen from natural sources and wastewater effluent: nutrient limitation on the Lower Truckee River
A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Environmental Science and Health
by Neil Bertrando Dr. R. G. Qualls/Thesis Advisor December, 2008
i
THE GRADUATE SCHOOL
We recommend that the thesis prepared under our supervision by NEIL BERTRANDO entitled Bioavailability of dissolved organic nitrogen from natural sources and wastewater effluent: nutrient limitation on the Lower Truckee River be accepted in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE
Dr. Robert G. Qualls, Advisor
Dr. Glenn Miller, Committee Member
Dr. Sudeep Chandra, Committee Member
Dr. John Sagebiel, Graduate School Representative
Marsha H. Read, Ph. D., Associate Dean, Graduate School
December, 2008
i Abstract Implementation of the Clean Water Act might be further refined to recognize differences in biological availability of different nitrogen species—nitrate-N, ammonium-N, and dissolved organic nitrogen (DON)—in the regulation of total nitrogen (TN) total maximum daily loads (TMDL). This study was conducted to assess the location and importance of N loading and associated nutrient limitation in the Truckee River across the urban gradient. To refine understanding of nutrient loading effects, periphyton nutrient limitation assays were performed across the urban gradient. Since the variety of constituents in DON have not been thoroughly identified and may vary, based on source and season, 67 day bioassays were performed to measure the fraction of DON that is mineralized or converted to particulate matter. Specifically, the bioavailable fraction of wastewater and urban DON was compared to “naturally derived” DON in the receiving river system. Seasonal bioassays in 2007 and 2008 demonstrated that wastewater derived DON consistently had a higher bioavailable fraction (20-51 %) than naturally derived DON, which was usually a small fraction. However during summer 2007 the fraction of bioavailable DON was similar for wastewater and natural sources (40 % and 41 %, respectively). DON derived from urban runoff had the highest degree of variation in bioavailability (3-70 %) as opposed to the more consistent bioavailability of wastewater DON or the summer pulse of bioavailblity in naturally derived DON . In the river, downstream from wastewater discharge, the bioavailable fraction of DON varied seasonally (0-42 %). Periphyton nutrient limitation assays demonstrated variations in significant limitation (p
