Volume 17 Number 6 1989 - BioMedSearch

Nucleic Acids Research

Volume 17 Number 6 1989 The nucleotide sequence of Atlantic salmon growth hormone cDNA

James Lorens, Audun H.Nerland, Rune Male, Ivar Lossius, Wenche Telle and Geir Totland Marine Genetics A/S, Laboratory of Biotechnology, University of Bergen, PO Box 3152, Aarstad, 5001 Bergen, Norway EMBL accession no. X14305 Submitted February 17, 1989

A cDNA library constructed from Atlantic salmon (Salmo salar) pituitary mRNA in AgtlO, was screened with oligonucleotide probes derived from conserved growth hormone sequences. Several clones were isolated and two were selected for sequencing. The 1169 bp nucleotide sequence (Figure 1) encodes a 210 amino acid prepeptide with a putative 22 amino acid leader sequence. cc-c cicccCAACOGACCCCCGGA

1 1

93 13

NA iG GGA CAA GTG TTT CM C-r ATG CCA GTC TTA CrG

A

M

G

Q

V

F

L

L

GTC AGT TGT TTT CTG AGC CAA GGG GCA GCG ATG GAA AAC CAA CGG CrC TTC AAC ATC GCG GTC AAC CGG GTG N V R F G A A E N R L F I A V N Q L S Q M C V S

TiT GAA GGT ACC CTG TiG CCT GAT GAA

CGC AGA CAG CTG AAC AAG ATA R Q L R K I N

M

C-A Q

TiC

V

P

L

L

CAT CTC CAC CTA L H L H CTG CTG GAC TTC L F L D

180 42

ATG GCr CAG AAG ATG TTC AAT GAC N A K F N D M Q

267 71

TGT MC TCT GAC TCC ATC GTG AGC cCA ATC GAC AAG C-T GAG ACT CAG AAG AGT TCA GTC CTG AAG CTG CTC CAT ATC TCT TiC CGT K S F L L H I R S P I D K L T K S S V L E Q I V N S D S C

F

E

G

T

L

L

P

D

E

F

354 100

CMc ATr GM TCC TGG GAG TAC CCT AGC CAG ACC Crc ACC ATC TCC MC AGC CrA ATG GTC AGA

441 129

C-C AGC GAC CTC AAA GTG GGC ATC AAC CIG C-C ATC AAG GGG AGC CAG GAT GGC GrA CMG AGC CTG GAT GAC AAT GAC TICT CAG CAG

528 158

CTG CCC L P

C-CC TAC GGG AAC TAC TAC CAG AAC CTG GGG GGC GAC GGC AAC GTC AGG AGG AAC TAT GAG TTG Tic GCC TGC TTC AAG AAG

615 187

GAC ATG N D

CAC- MG

L L

I S

E D P H

S

L Y K

W

K G

E V N

Y G Y

P

I Y

S

N Q

Q

L N

T L L

L I G

T K G

I

G D

S

N

S

Q N

G

S D V

GTC GAG ACC TAC CTG ACC GTC GCC AAG TGC AGG AAG TCA R K S A K V T Y L T V C E

L G R

CT-G L

M

V R

V L

N

R

S Y

AAC TCC AAC CAG ATC TCT GAG AAG I S E K S N Q N

L E

D L

GAG GCC AAC TGC ACT C A N E

D L

N A

S

D

F

C

Q

K

Q

K

C-G TAG ACGlGGGc-IGGAGAG L

-

705 820

935

1050 1165

TCTiC

Figure 1.

The encoding sequence demonstrates a high degree of similarity with salmonid growth hormone sequences (Table 1). Table 1. Degree of identity (%)

Chum salmon Coho salmon Rainbow trout

Nucleotides (coding region) 95.6 97.0 96.5

Signal peptide (aa 1-22) 100 100 91

Hormone (aa 23-210) 96.3 93.8 96.3

Ref. 1 2 3

The 5'-untranslated region is nearly identical among the salmonids, while the 3'-untranslated region is relatively less conserved. Genomic cloning has revealed two distinct sgh genes (unpublished results). This sequence corresponds to the locus denoted sgh I.

References: T Sekine S., et al. (1985) Proc Natl Acad Sci USA 82:4304-4310 2. Nicoll, C.S., et al. (1987) Gen Comp Endocrinol 68:387-399 3. Agellon, L.B. and Chen, T.T. (1986) DNA 5:463-471 2352