Evidence for Boundary Lipid in Membranes

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the supernatant and pellet were discarded and the fluffy middle layer was retained. After the final ... Micrograph taken by Mr. William. Colquhoun. (Bar = 0.1 ,um).
Proc. Nat. Acad. Sci. USA Vol. 70, No. 2, pp. 480-484, February 1973

Evidence for Boundary Lipid in Membranes (membranous cytochrome oxidase/spin label/electron spin resonance)

PATRICIA C. JOST*, 0. HAYES GRIFFITH*, RODERICK A. CAPALDIt, AND GARRET VANDERKOOIt *Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, Ore. 97403; and tInstitute for Enzyme Research, University of Wisconsin, Madison, Wis. 53706

Communicated by V. Boekelheide, December 4, 1972 ABSTRACT Cytochrome oxidase (EC 1.9.3.1) isolated from beef-heart mitochondria with an appropriate phospholipid content forms vesicular structures. Lipid-protein interactions in this model membrane system were studied with thelipid spin label, 16-doxylstearic acid. As the phospholipid/protein ratio is varied, two spectral components are observed. At low phospholipid/protein ratios (

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