0.000871 cell,projection,assembly. 0.000298 endopeptidase,inhibitor,activity. 0.000883 regulation,of,wound,healing. 0.000319 positive,regulation,of,response ...
Allatostatin A receptor 1 (comp36292) Allatostatin C receptor 1 (comp29390) Allatostatin neuropeptide (comp11680) Allatostatin neuropeptide (comp23606) Arylalkylamine N-acetyltransferase (comp26883) Arylalkylamine N-acetyltransferase (comp34439) Diapause hormone receptor (comp29733) Dopamine receptor (comp31794) Ecdysis triggering hormone receptor (comp36183 a) Ecdysis triggering hormone receptor (comp36183 b) Ecdysone receptor (comp16498) -
-
* *
* Juvenile hormone epoxide hydrolase (comp32778) Juvenile hormone epoxide hydrolase (comp34461) Melatonin receptor (comp20872) Methoprene-tolerant (comp27650) Prothoracicotropic hormone (comp22961) - * RACK1 (comp30484) Serotonin receptor (comp28638) Serotonin receptor (comp33893) Sex peptide receptor (comp13465) Sex peptide receptor (comp245397) Sex peptide receptor (comp30920) Sex peptide receptor (comp34224) Sex peptide receptor (comp36082) Sex peptide receptor (comp36800) Takeout/ JHBP (comp25385) Takeout/ JHBP (comp25618) Takeout/ JHBP (comp26377) Takeout/ JHBP (comp26537) Takeout/ JHBP (comp28071) Takeout/ JHBP (comp29016) Takeout/ JHBP (comp29764) Takeout/ JHBP (comp30339) Takeout/ JHBP (comp30385) Takeout/ JHBP (comp30466) Takeout/ JHBP (comp30593) Takeout/ JHBP (comp33588) -Takeout/ JHBP (comp35402) Tyramine beta-hydroxylase (comp33029) I
>
0
0
0
.c
0 .c 0..
z
a given contrast.
Table S1. Summary statistics of transcriptome assembly in Trinity.
Summary Sta)s)cs Unique Reads Transcripts Components Minimum Read Length Maximum Read Length Mean Read Length Median Read Length N50
61.5 M 78,236 40,811 201 17,430 1,136 691 1,918
Supplemental Table 2. Overlapping differentially expressed genes between daily and seasonal time courses. Candidate PDD Interval PDD Interval PDD Interval
Levy Comp comp9904_c0_seq1 comp48611_c0_seq1 comp36669_c0_seq10
Wadsworth Comp comp34828_c0_seq1 comp19186_c0_seq1 comp25161_c0_seq1
Gene Symbol CG30427 CBG16133 Magu
DE Diapause D1 D1 D1
PDD Interval comp35947_c0_seq1 PDD Interval comp27771_c0_seq2 PDD Interval comp26528_c0_seq1
comp9449_c0_seq1 comp19707_c0_seq1 comp18687_c0_seq1
GAPsec CG10338 Plod
D1 D7 D7
PDD Interval PDD Interval PDD Interval Hormonal Hormonal Hormonal Hormonal
comp21518_c0_seq1 comp32386_c0_seq3 comp31831_c0_seq2 comp26537_c0_seq1 comp30466_c0_seq1 comp30593_c0_seq1 comp33588_c0_seq1
comp117543_c0_seq1 comp78006_c0_seq1 comp9302_c0_seq1 comp33893_c0_seq1 comp28293_c0_seq1 comp22834_c0_seq1 comp188240_c0_seq1
Ttc39b Sca Rngo to to to to
D7 D7 D7 D1 D1 D1,D7 D7
Hormonal
comp36183_c1_seq1
comp26395_c0_seq1
ETHR
D7
DE Daily Hr4 P,Hr1.3,Hr4 Hr4
Gene Name Fatty acyl-CoA reductase NA NA GTPase-activating, SECISP,Hr1.3,Hr4 dependent read-through Hr1.3,Hr4 NA P,Hr1.3,Hr4 Procollagen lysyl hydroxylase Tetratricopeptide repeat protein 39B P P,Hr4 Scabrous Hr1.3 Rings lost Hr4 Takeout Hr1.3,Hr4 Takeout Hr1.3 Takeout P,Hr4 Takeout ecdysis triggering hormone Hr1.3,Hr4 receptor
Function Determination of lifespan NA Regulation of life span, BMP signalling Translation NA Collagen IV biosynthesis NA Neural patterning and interacts with the Notch pathway Development Juvenile hormone binding protein Juvenile hormone binding protein Juvenile hormone binding protein Juvenile hormone binding protein Ecdysone binding
Table S3. Summary of GO term enrichment among significantly upregulated genes within each strain at each time point.
E"Strain Photophase
Process
p"value
0 2 light,absorption reactive,nitrogen,species,metabolic,process
0.000846 0.000846
5 pigmentation developmental,pigmentation phenol"containing,compound,metabolic,process cuticle,pigmentation regulation,of,melanization,defense,response
0.000538 0.000538 0.000545 0.000697 0.000903
Process
p"value
Hour1
Hour4
Z"Strain
Photophase
Hour1
Hour4
6 positive,regulation,of,wound,healing cell,projection,assembly regulation,of,wound,healing positive,regulation,of,response,to,wounding lamellipodium,assembly lamellipodium,organization 5 nucleobase,biosynthetic,process DNA,metabolic,process de,novo,pyrimidine,nucleobase,biosynthetic, process pyrimidine,nucleobase,biosynthetic,process nucleobase,metabolic,process 0
0.000183 0.000298 0.000319 0.000515 0.00068 0.00068 0.000184 0.000443 0.000671
Function 1 Hydrolase"activity 5 cation:,chloride,symporter,activity anion,transmembrane,tranporter,activity inorganic,anion,transmembrane,tranporter,activity chloride,transmembrane,transporter,activity secondary,active,transmembrane,transporter, activity 0
Function
p"value
Component
p"value
0 0.00062 0 0.0000954 0.000215 0.000271 0.000965 0.000983 0
p"value
Component
2 catalytic,activity endopeptidase,inhibitor,activity
0.000871 0.000883
3 structural,constituent,of,chitin"based,cuticle structural,constituent,of,cuticle 2"methylpropanoyl,transfering,activity
2 0.0000721 extracellular,matrix 0.000124 lipoamide,complex 0.000559
1 carboxylic,ester,hydrolase,activity
0.000608
p"value
0
0.000671 0.000891 0
0.000253 0.000559
