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Fragment Length Distribution

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Figure 2: The sequencing error model summary plot generated by rsem-plot-model after running RSEM on the RNA-Seq data from SRA experiment SRX018974 ...
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Fragment Length Distribution

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Fragment Length Mean = 208.2 , Std = 29.3 Figure 1: The fragment length distribution plot generated by rsem-plot-model after running RSEM on the RNA-Seq data from SRA experiment SRX018974.

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Observed Quality vs. Phred Quality Score



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Phred Quality Score

Figure 2: The sequencing error model summary plot generated by rsem-plot-model after running RSEM on the RNA-Seq data from SRA experiment SRX018974. The Phred quality scores are the scores produced from the sequencer and the “observed quality scores are estimates of the actual quality of the bases. On this data set, RSEM learns that the sequencer-produced quality scores are generally overestimates, although some of this difference can be attributed to the sample transcriptome having polymorphisms relative to the reference sequences.

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Number of fragments

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Histogram of Alignments per Fragment

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Alignments per fragment

Figure 3: A diagnostic plot of the number of alignments per fragment generated by rsem-plot-model after running RSEM on the RNA-Seq data from SRA experiment SRX018974. This plot gives the user an idea of how common multireads are for a given data set.

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