folding, antigen-antibody recognition, stabilization of enzyme-substrate complexes, ... Fundamentals and Applications in Biomedical Engineering. 605. Fig. 1.
cal repertoire of protein chemistry as has affinity chromatography, although a ... can be very effectively adsorbed but an elution in a native state is often practi-.
Gel filtration (also referred to as size exclusion chromatography, SEC) separates ... Unlike ion exchange or affinity chromatography, molecules do not bind to the.
Online Coupling of Reverse-Phase and Hydrophilic Interaction. Liquid Chromatography for Protein and Glycoprotein. Characterization. Maggie P. Y. Lam, S. O. ...
Sep 29, 2004 - Key words: Gene therapy, hydrophobic interaction chromatography, plasmid, purification ... fast protein liquid chromatography (FPLC) system.
SPAC gels like thiophilic gel (T-gel), mercapthylene pyridine- derivatized agarose gel (MP), tricyanoaminopropeneâdivinyl sulphone agarose gel (DVS-TCP), ...
Jul 24, 2008 - Hydrophobic interaction chromatography (HIC) has been developed as a ... green fluorescent protein; hydrophobic interaction chromatography; ...
Oct 3, 2011 - [15] Hydrophobic interaction chromatography is rela- ..... http://www.fm.uit.no/info/imb/amb/courses/bio360s/pdf/hydrophobic_interaction. 16.
Jul 14, 1976 - metho-p-toluene sulphonate from the Aldrich Chemi- cal Corporation, Inc. .... thought to exert its toxic action (Allison et al., 1966), it has no ...
The hydrophobic interaction, the tendency for nonpolar molecules to aggregate in solution, is a major driving force in biology. In a direct approach to the physical ...
May 30, 2015 - 1Maharani's Science College for Women, Bangalore University, ... University of Mississippi/Northwest Mississippi Community College, DeSoto ...
May 30, 2018 - unfolding upon adsorption by isothermal titration calorimetry ... to Toyopearl Butyl-650 M was studied with isothermal titration calorimetry to ...
these conditions the gel usually shrinks up to 50% [Demiroglou & Jennissen,. 1990]. ...... Fausnaugh, J.L., Kennedy, L.A and Regnier, F.E. (1984) Comparison of ...
[P1.110]. Toward therapeutic plasmids purification by hydrophobic interaction membrane chromatography. M. Mateus*, L. Raiado-Pereira, D.M.F. Prazeres.
Nov 3, 1995 - attributed primarily to removal of nonpolar surface area of the molecule .... stationary phase consisting
Jul 15, 2010 - chromatography (HILIC) online in a micro-flow scheme. In this approach ..... In contrast, those retained by the HILIC system were either short ..... Manual examination identified further glycopeptide signals such as protonated y11-. Ma
1Department of Pharmacy, IIMT College of Medical Sciences, Meerut, Uttar Pradesh, India. 2Department of Pharmacy, Annamalai. University, Chidambaram ...
parameters tested and method is free from interference of the other active ... 2006, 941. 2. Sean C. Sweetman., Martindale: The complete drug reference, 37th.Missing:
Jun 24, 2013 - recrystallization fraction variable, fRX; and (8) the fraction variable, f ..... annealing temperatures. 10. 100. 1000. 160. 180. 200. 220. 240. 260.
Feb 20, 2007 - water, as a model for hydrophobic association. A stable configu- ... simulations but agree well with a simple hydrophobic burial model which is ...
(PEG) 6000 (Carbowax 6000;Union Carbide, New. York, N.Y.) and 6.2% (wt/wt) dextran T500 (Phar- macia Fine Chemicals AB, Uppsala, Sweden) in 0.03.
bovine S100B calibrator with the various human recom- binant S100 proteins in concentrations of 10â30 μg/L. There was no interference/cross-reactivity by the ...
can later be used as the basis for specifying a new graphical user interface tailored to the ... an instance of the screen that corresponds to the node. An edge may be ...... FL, Nov 10, 1993. [10] H.A. Mueller, M.A. Orgun, S.R. Tilley, & J.S. Uhl:.
philic/hydrophobic mechanism requires large wettability difference. ... The relationship between the surface area and the contact angle is defined by Wenzel's equa- .... [2] âSurface Adhesion Control of Patterned Perfluoro Polymer for Release ...
Hydrophobic Interaction. Chromatography(HIC). Hydrophobic Interaction and Reverse Phase. Chromatographies. THE 2ND SUMMER SCHOOL IN.
P7
Hydrophobic Interaction and Reverse Phase Chromatographies
THE 2ND SUMMER SCHOOL IN INTERDISCIPLINARY SCIENCES 21-24 september 2009
Presenter: Z. Behzadian Supervised by Dr.R.Yousefi (Assistant Professor of Biochemistry) Co- Supervisor:Dr.H.R.Karbalaei (Assistant Professor of Biochemistry) Department of Biology,College of Sience,Shiraz University,Shiraz 71454,Iran
Hydrophobic Interaction Chromatography(HIC)
Stationary phase of HIC
HIC is temperature sensitive
Hydrophobic residues of proteins usually are buried internally. The number and distribution of hydrophobic residues are varied on the surface of proteins which can be used in HIC. When a hydrophobic region of a biopolymer binds to the surface of a mildly hydrophobic stationary phase, hydrophilic water molecules are effectively released from the surrounding hydrophobic areas causing a thermodynamically favorable change in entropy.
Reverse- phase chromatography (RPC)
Temperature and ionic strength play strong roles in HIC.
Reverse- phase chromatography includes any chromatographic method that uses a non-plar stationary phase.
Ammonium sulfate, by virtue of its good salting-out properties and high solubility in water is used as an eluting buffer
Principles of RPC
Hydroponic interactions is ionic strength sensitive Elution of bind protein in HIC
