Improved Detection of Metastases by Step Sectioning and Immuno ...
Recommend Documents
Breast surgery (radical mastectomy or breast conserving therapy) with axillary lymph node dissection (ALND) is the most common method for breast cancer ...
Abstract. Clinical usefulness of sentinel lymph node (SLN) biopsy has been demonstrated in the management of early vulvar cancer. However, what constitutes ...
Keywords: Immuno-PCR, STV, biotin, monoclonal antibody, D-dimer, canine ... including the avidity of the ligand, the amount of non-specific binding of the.
Mar 27, 2006 - samples from healthy volunteers. ... None of the volunteers had taken antibiotics or other drugs known to .... Orange cells have hybridized with.
A novel method based on immuno-chemiluminescence and image analysis using charge coupled device (CCD) for .... mined by adding 1 mM luminol (100 μl); 0.1 mM urea H2O2 ..... nol and hydrogen peroxide (Bostick and Hercules, 1975).
HFQRS intensity analysis. Results: Thirty-two patients (31%) had significant CAD. .... Incomplete study with exercise intolerance. (n=40) ... RBBB. (n=17). IVCD. (n=2). Eligible patients (n=440). Patients enrolled for final analysis (n=175). Fig. 1.
Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD,. Ho PL, Coviello GM, ... Nakayama J, Saito M, Nakamura H, Matsuura A and. Ishikawa F. (1997). Cell ...
Jan 14, 2017 - molecular weight cut-off of 100 kDa (100 kDa MWCO,. Millipore .... Thermowell 96 Well Polycarbonate PCR Microplate (Corning. Costar Cat.
May 21, 2010 - ics of Air Pollution (Wiley, New York, 1986). 22. ... cation capability and specificity of PCR, this immuno-PCR technology has a sensitivity.
ther, our work establishes ID-seq as a fiexible solution for large-scale high- .... 1 Immuno-detection by sequencing (ID-seq) technology development. a Concept ...
... Section of Thoracic. Surgery, Suite 300 Kaufmann Bldg., 3471 Fifth Ave., Pittsburgh, PA 15213, USA. ... CA). Tissue lysis and homogenization were performed.
Nov 21, 2013 - Keywords: colorectal cancer; 5-aminolevulinic acid; lymph node ..... Le Frere-Belda, M.A.; Bats, A.S.; Gillaizeau, F.; Poulet, B.; Clough, K.B.; Nos ...
Jun 25, 2009 - U.S.. Environmental Protection Agency method 1622/23 provides an ... Improved assessment of the risk for designated sources or in treated .... Inc., Burlington, Ontario, Canada), 0.1 g kanamycin liter 1, 100,000 U penicillin ... oocyst
Jul 23, 2014 - effective analytics to guide clinical assessors towards likely drug safety ... tional reporting; the number of reports with free-text descriptions of the ...
Apr 22, 2015 - cient optimal cutting temperature compound (OCT) embedding media to fully submerse the scaffolds. We have found that Fisher, flat-bottom ...
Nov 23, 2016 - This protocol modifies a paraffin-based sectioning technique and improves ..... Cut the 2nd and 3rd axillary bud tissue slices (10 µm thickness) ...
MRI Ñ USPIO had surplus value in one patient. Not all lymph node stations could be compared due to unforeseen explorations. No adverse effects occurred ...
ABSTRACT. We propose sliding-window multiedge detectors and reflectivity es- timators for complex SAR images. The novel detectors and estima- tors allow to ...
Jul 19, 1989 - 7W73. Franklin, IA. and Dusseault, MB. (1989) Rock Engineering, Chapter 13: Blasting, pp. 415â452,. McGraw-Hill Book, Company. New York.
after a monochromatic point source for producing a pair of twin, fully coherent, virtual point sources. The resulting interference fringes are projected into the 3D ...
rus (PDV), Apple mosaic virus (ApMV), and American plum line pattern virus ... in infected orchards (Stouffer and Uyemoto, 1976). Detection of ilarviruses is ...
âOpenCV Binary folderâ should be defined in Windows environmental path ... In Visual Studio 2010 C++ developer, create new application by following one of ...
Easy German Step-by-Step features: A unique âbuilding-blockâ approach to mastering grammar, verbs, and. vocabulary E
Improved Detection of Metastases by Step Sectioning and Immuno ...
positive lymph nodes increases (De Boer et al., 2010). Breast surgery (radical mastectomy or breast conserving therapy) with axillary lymph node dissection ...
DOI:http://dx.doi.org/10.7314/APJCP.2013.14.10.5731 Better Metastatic Detection with Step Sectioning of Lymph Nodes for Breast Cancer
RESEARCH ARTICLE Improved Detection of Metastases by Step Sectioning and Immuno-Histochemical Staining of Axillary Sentinel Nodes in Patients with Breast Carcinoma Fereshteh Ensani1*, Ladan Enayati1, Afsaneh Rajabiani2, Ramesh Omranipour1, Nasrinalsadat Alavi3, Sara Mosahebi3 Abstract Background: The object of this study was to examine whether a new protocol including step-sectioning and immunohistochemistry (IHC) staining of axillary sentinel nodes (SN) would lead to detection of more metastases in patients with breast cancer. Materials and Methods: Sixty-nine tumor free sentinel lymph nodes were examined. Step frozen sectioning was performed on formalin fixed SN and stained both by hematoxylin and eosin (H and E) and cytokeratin markers using IHC. Any tumoral cell in IHC stained slides were considered as a positive result. Metastases up to 0.2 mm were considered as isolated tumor cells and 0.2 up to 2 mm as micrometastasis. Results: Mean age of the patients was 48.7±12.2 years. Step sectioning of the SN revealed 11 involved by metastasis which was statistically significant (p0.05 3±1.7 3.5±1.8 Presence of Vascular invasion 15/69 9/11 0.001* 14/69 10/15 Presence of Perineurial invasion 6-69 5/11 0.01* 6/69 5/15 *Immunohistochemistry staining, **p value 0.05
