A simple, scalable, isothermal process for nanoemulsion formation
Srikanth Kakumanu 2 Mathieu Schmitt 1 Xiaoyin He 1 Carl Beckett 1 James Laugharn
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PROCESS TECHNOLOGIES Covaris, Inc. • 14 Gill Street, Unit H, Woburn, Massachusetts 01801-1721 USA Tel: +1 781-932-3959 • Fax: +1 781-932-8705 • Email:
[email protected] Web: www.covarisinc.com
High Performance Emulsification using Adaptive Focused Acoustics
1. Covaris, Incorporated, Woburn, MA, 01801, USA
[email protected] 2. Novagali Pharma, Genavenir IV, F-91058 Evry cedex, France.
Introduction
Continuous FLOW process
Nanoemulsions are a very effective vehicle for both the encapsulation of and the delivery of FIGURE 1: Key unit operations for preparing emulsions biologically active ingredients (1). However, High Shear Mixer current methods for the formation of nanoemulsions include extremely high heat, high shear force methods such as Microfluidizers, and complex chemistry, high temperature, low energy methods such as Self Oil Phase Aqueous Phase Course Emulsion High Power/High Control Fine Emulsion Assembling Nano Emulsions (SANE) are Coarse (1-4) technically limiting . Findings show that the Filtration (5μl) Key Process Parameters use of focused acoustic energy is very effective • Rate of Mixing • Temprature to form nanoemulsions across a wide range of • Rate of Cooling • Pressure sample volumes from under 0.50ml through • Number of Cycles • Sterilization duration continuous process flow. The ability to Heat Sterilzation formulate monodisperse, nanoemulsions, with average particle sizes down to 20nm was previously demonstrated with a poly-dispersity index below 0.100. All results were achieved in very fast process time and with sample temperatures held below room temperature in all cases (e.g.,
