1Alex and Simona Shnaider Laboratory in Molecular Oncology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Joseph & Wolf Lebovic Health ...
Transglutaminase II Expression In Invasive Ductal Carcinomas Jasmeet 1Alex
1 Assi ,
Gunjan
1 Srivastava ,
Ajay
1 Matta ,
Martin C.
2,3 Chang ,
Paul G.
1,2,4,5,6 Walfish
and Ranju
1,2,4,6 Ralhan
and Simona Shnaider Laboratory in Molecular Oncology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Joseph & Wolf Lebovic Health Complex, Toronto, Ontario, Canada, 2Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada, 3Department
of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada. 4Joseph and Mildred Sonshine Family Centre for Head and Neck Diseases, Mount Sinai Hospital, Toronto, Ontario, Canada, 5Department of Medicine, Endocrine Division,
Mount Sinai Hospital and University of Toronto, Toronto, Ontario, Canada , 6Department of Otolaryngology – Head and Neck Surgery, Mount Sinai Hospital, Toronto, Ontario, Canada.
Introduction
Results Archived tissue blocks of 168 IDCs and 40 normal breast tissue (mean age: 59 years; range: 29 to 89 years) undergoing curative cancer surgery during the period 2000 - 2002 were retrieved from the Mount Sinai Hospital. Comprehensive clinicopathologic data were available in digital databases for each of these cases including demography, clinical tumor staging (American Joint Committee on Cancer staging guidelines), histological grade, clinical outcome including recurrence - local, regional, locoregional or distant; treatment, subsequent management and disease status at last clinical review. The hematoxylin and eosin (H & E) stained slides of these cases were reviewed and tumor tissues confirmed by the pathologist (MC). These slides were stained for TG2 by immunohistochemistry (Figure 1). These sections were scored based on percentage of cells stained and intensity as follows: 0, < 10% cells; 1, 11-30% cells; 2, 31-50% cells; 3, 51-70% cells; and 4, >70% cells showed immunoreactivity. Sections were also scored semiquantitatively on the basis of intensity as follows: 0, none; 1, mild; 2, moderate; and 3, intense. Finally, a total score (ranging from 0 to 7) was obtained by adding the scores of percentage positivity and intensity for each of the tissue sections.
Figure 2. TG2 Staining pattern A B
Figure 4. Boxplot analysis of stromal TG2 A B p
