Metformin potentiates the benefits of dietary restraint ...
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Metformin potentiates the benefits of dietary restraint ...
Supplementary material corresponding to the manuscript:
Metformin potentiates the benefits of dietary restraint: a metabolomic study Marta Riera-Borrull 1,2, Anabel García-Heredia 1, Salvador Fernández-Arroyo 1,3, Anna Hernández-Aguilera 1, Noemí Cabré 1, Elisabet Cuyàs 3,4, Fedra LucianoMateo 1, Jordi Camps 1, Javier A. Mendendez 3,4 and Jorge Joven 1,5*.
Supplementary material and methods S1: Food intake measurement Food intake measurement was performed as previously reported [1]. Mice were group housed and given a known, pre-weighed amount of food in their home cage hopper. Once a week, the remaining food is weighed. Then, new amount of food is administered. Calculations were performed as follow: Food intake = Food provided – Food ingested – food wasted. Food intake measurements were divided by the number of animals per home cage, so assuming a meanintake per animals, and represented in g, although it can be easily converted to kcal, by using data on Table S1. [1] Ellacott KLJ et al., Cell Metab, 2010.
1
Table S1: Nutritional composition of experimental diets.. CD (Chow diet)
