Osteopontin attenuates aging-associated phenotypes ...

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ST-HSC. MPP. 0. 20. 40. 60. % o. f d o n o. r-d e riv e d. L. S. K s. BM 20wks ... PN. K. O c n tr. O. PN. K. O. A. O. PN. K. O. B. Yo u n g c n tr. A. Appendix Figure S3.
Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells! Guidi et al.! Appendix! Table of contents:! Appendix Figures S1-S3!

A

Ly5.2 recipients!

Young BM! 2x10^6 BM cells! !

Young

D

C! *

*

60 40 20

60

PB 20wks

60

40

20

0

0

Y/Yin Y Y/KO in Y Y/O in Y

* %CD45.1 cell

80

Y-KO O

% of donor-derived LSKs

B! 100

% donor derived cells

20 wks after secondary transplantation !

Y

40

20

0 LT-HSC

ST-HSC

MPP

BM 20wks

B cells

T cells

myeloid

PB 20wks

Appendix Figure S1. Aging associated phenotypes of LT-HSC are attenuated upon secondary transplantation in young mice (A) Schematic representation of the experimental setup (B) Frequency of young donor contribution (Ly5.1+ cells) to total WBC in PB in young recipients (Ly5.2+) mice. (C) Frequency of young LT-HSC, ST-HSC, and MPP cells in BM among donor-derived LSKs cells in young recipients (Ly5.2+) mice. (D) Frequency of young B cells (B220+), T cells (CD3+), and myeloid cells among donorderived Ly5.1+ cells in PB in young recipients (Ly5.2+) mice (n=4-10 mice per group). Shown are means values + 1 SEM, *p