Patch-clamp recording in brain slices with improved slicer technology
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In this context the mechanics of the tissue slicer are an important factor. Ideally, a tissue ... Keywords Adjustable vibroslicer · Auto-resonant mech- ... Received: 4 May 2001 / Revised: 1 June 2001 / Accepted: 12 September 2001 / Published online:
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Patch-clamp recording in brain slices with improved slicer technology
Oct 17, 2001 - Ideally, a tissue slicer should generate large-amplitude and high-frequency move- ments of the cutting blade in a horizontal axis, with minimal ...
Pflügers Arch - Eur J Physiol (2002) 443:491–501 DOI 10.1007/s00424-001-0735-3
INSTRUMENTS AND TECHNIQUES
J. R. P. Geiger · J. Bischofberger · I. Vida · U. Fröbe S. Pfitzinger · H. J. Weber · K. Haverkampf · P. Jonas
Patch-clamp recording in brain slices with improved slicer technology
Abstract The use of advanced patch-clamp recording techniques in brain slices, such as simultaneous recording from multiple neurons and recording from dendrites or presynaptic terminals, demands slices of the highest quality. In this context the mechanics of the tissue slicer are an important factor. Ideally, a tissue slicer should generate large-amplitude and high-frequency movements of the cutting blade in a horizontal axis, with minimal vibrations in the vertical axis. We developed a vibroslicer that fulfils these in part conflicting requirements. The oscillator is a permanent-magnet-coil-leafspring system. Using an auto-resonant mechano-electrical feedback circuit, large horizontal oscillations (up to 3 mm peak-to-peak) with high frequency (≈90 Hz) are generated. To minimize vertical vibrations, an adjustment mechanism was employed that allowed alignment of the cutting edge of the blade with the major axis of the oscillation. A vibroprobe device was used to monitor vertical vibrations during adjustment. The system is based on the shading of the light path between a lightemitting diode (LED) and a photodiode. Vibroprobe monitoring revealed that the vibroslicer, after appropriate adjustment, generated vertical vibrations of
