Possible strategies for conditional genome engineering using the ...
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Possible strategies for conditional genome engineering using the ...
Supplemental Figure 1: Possible strategies for conditional genome engineering using the loxPint module. The reconstitution of the loxPint module after RAP ...
Supplemental Figure 1: Possible strategies for conditional genome engineering using the loxPint module. The reconstitution of the loxPint module after RAP treatment allows the conditional fusion or deletion of domains. This opens new avenues for conditional epitope or enzyme tagging (for example with biotin ligase A (BirA)), conditional domain swaps, introduction of point mutations or perfect complementation, that is, replacing a genetic element with an identical copy. Using CRIPSR the introduction of two loxPint modules into an open reading frame to delete only parts of a gene will be feasible. Yellow hexagons represent stop codons.
