Rapid Method for Determination of β-Carotene in ... - Springer Link
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Rapid Method for Determination of β-Carotene in ... - Springer Link
Oct 31, 2010 - Keywords: β-carotene, rapid determination, ultra high performance liquid ..... Rapid isocratic HPLC analysis of beta-carotene in red peppers.
Food Sci. Biotechnol. 19(5): 1199-1204 (2010) DOI 10.1007/s10068-010-0171-2
RESEARCH ARTICLE
Rapid Method for Determination of β-Carotene in Foods Using Ultra High Performance Liquid Chromatography Jaeho Ha, You-Shin Shim, Hye-Young Seo, Hyun-Jin Nam, Masahito Ito, and Hiroaki Nakagawa
Abstract The ultra high performance liquid chromatography (u-HPLC) method for determination of β-carotene in foods was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on a reversed column C18 (particle size 2 µm, i.d. 2 mm, length 50 mm), followed by ultra violet (UV) detection at 450 nm. The recovery of β-carotene was more than 84.4% and the limit of detection and limit of quantitation of u-HPLC analysis were 0.28 and 0.85 µg/mL for β-carotene with butylated hydroxytoluene (BHT) and 0.62 and 1.89 µg/mL for βcarotene without BHT, respectively. The calibration graph for β-carotene was linear from 0.1 to 25.0 µg/mL for uHPLC. The intra- and interday precisions (relative standard deviations) were
