Relativity of Platelet count with automated cell counter ...
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Relativity of Platelet count with automated cell counter ...
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Original Article
MAJMAAH JOURNAL HEALTH SCIENCE, 2014-vol 2, Issue 1
Relativity of Platelet count with automated cell counter and Manual Haemocytometer using Peripheral Blood Smear Purnendu Sinha1, Moattar Raza Rizvi2, *R.K.Choudhary2,
ABSTRACT
الملخص
Background: Obtaining accurate, precise, and reliable platelet counts as a reference for the calibration of hematology analyzers has been a continuing problem due to varying results. We proposed the relativity of platelet count by Automated Cell Counter and Manual Haemocytometer with Peripheral smear for focusing the accuracy and & cost effectiveness, without compromising the accuracy of test results. Material & Methods: Seventy-Five blood samples in EDTA-anticoagulant vacutainer tubes were obtained and randomly divided into 3 group; Group I-normal control (n = 25), Group IImild thrombocytopenia (n = 25) and Group III-severe thrombocytopenia (n = 25). Each blood sample had two peripheral blood smears made and stained on an automatic slide stainer. Peripheral blood smears were analyzed for platelet count by manual microscopy, an automated hematology analyzer Beckman Counter Diff 2 in all groups. The agreement between the two methodologies were assessed using the paired t-test and correlation coefficient analyses. Results: The coefficient of variation values significantly differed in Group C and were high for Automated cell counter. High postitive correlation was also observed between Automated cell counter values and manual method (r=0.82, p
