S1 Supporting Information
Useful Oriented Immobilization Of Antibodies On Chimeric Magnetic Particles: Direct Correlation of Biomacromolecule Orientation with Biological Activity By AFM Studies.
Marzia Marciello1, Marco Filice2, David Olea2, Marisela Velez2, José M. Guisan*2, Cesar Mateo*2
* Co-corresponding authors: Dr. Cesar Mateo / E-mail:
[email protected] Phone: +34-915854768/ Fax: +34-915854760 Dr. José M. Guisán/ E-mail:
[email protected] Phone: +34-915854809/ Fax: +34-915854760
Index
Figure S1…………………………………………………………………………………………...S2 Figure S2..………………………………………………………………………………………….S3 Scheme S1…………………………………………………………………………………………..S4 Scheme S2…………………………………………………………………………………………..S5
S2
Figure S1.
Effect of ionic strength on nonspecific adsorption of horseradish peroxidase on
aspartic-blocked M-RML-OS derivative. (mean values ± se; n = 3).
S3
Figure S2. Dimensional analysis of a) IgG (PDB file: 1IGY) and b) horseradish peroxidase (HRP) (PDB file: 1GWU).
S4
Scheme S1. Preparation of pure M-RML by means of solid phase strategy. I) Lipase hydrophobic reversible immobilization. II) Amino-epoxidation of hydrophilic side of adsorbed lipase. III) Desorption of modified lipase and detergent removal.
S5
Scheme S2. Immobilization of IgG on M-RML-OS and surface inhertization with aspartic acid. I) a) Two-step orienting immobilization of antibody on lipase surface; b) Epoxy groups blocking by incubation with aspartic acid
