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S1 Table. X-ray data collection and refinement statistics for the CpGH31 CBM32s. Data collection statistics. CBM32-1 (apo). CBM32-2 (GalNAc). CBM32-3 (apo).

S1 Table. X-ray data collection and refinement statistics for the CpGH31 CBM32s. Data collection statistics Wavelength (Å) Beamline Space group Resolution range Cell dimension α, β, γ, (°) Rmeas Completeness (%) Redundancy Total reflections Unique reflections

CBM32-1 (apo)

CBM32-2 (GalNAc)

CBM32-3 (apo)

CBM32-3 (galactose)

CBM32-3 (GalNAc)

1.033 APS 23-ID-B C2221 30.00-1.35 (1.40-1.35) 55.62, 55.67, 88.70 90.00, 90.00, 90.00 0.063 (0.45) 98.9 (97.7) 23.8 (4.2) 5.7 (5.1) 171557 30335

0.979 CLS 08B1-1 P22121 38.41-2.00 (2.05-2.00) 48.30, 84.75, 86.17 90.00, 90.00, 90.00 0.16 (0.52) 99.9 (100) 13.7 (7.8) 14.0 (14.1) 345760 24623

1.055 NSLS X6A P212121 50.00-1.58 (1.64-1.58) 38.76, 52.56, 70.17 90.00, 90.00, 90.00 0.042 (0.084) 98.9 (91.0) 34.7 (26.6) 7.8 (7.6) 157870 20155

0.98 NSLS X6A P43212 50.00-1.48 (1.53-1.48) 62.33, 62.33, 85.35 90.00, 90.00, 90.00 0.057 (0.38) 99.9 (99.2) 31.5 (6.3) 8.4 (8.0) 242452 28797

0.98 SSRL 14-1 P43212 42.89-2.50 (2.60-2.50) 60.549, 50.549, 83.69 90.00, 90.00, 90.00 0.114 (0.513) 100.0 (100.0) 14.3 (3.8) 6.0 (6.2) 35305 5836

18.9 20.7

19.2 22.1

16.1 19.4

17.6 20.2

17.4 22.1

0.006 1.066

0.020 1.928

0.024 2.248

0.006 1.042

0.007 1.016

15.471 23.924 10.335

14.906 16.384 26.716 30.178 8.660

8.266 20.399 9.189

14.436 26.655 28.518 15.400

28.966 34.197 44.594 39.150

1152 114 2

1175 1156 352 50 2

1277 193 2

1257 229 24 1

1235 52 30 1

97.1 2.9 0.0

98.0 2.0 0.0

98.1 1.9 0.0

96.9 3.1 0.0

96.8 3.2 0.0

4LPL

4UAP

4LQR

4LKL

4P5Y

Refinement statistics Rwork (%) Rfree (%) r.m.s.d. Bond lengths (Å) Bond angles (°) Average B-factors (Å2) Protein atoms chain A Protein atoms chain C Water molecules Ligand Cations Number of atoms Protein atoms chain A Protein atoms chain C Water molecules Ligand Cations Ramachandran statistics Most favoured (%) Additional allowed (%) Disallowed (%) Protein Data Bank code

*Numbers in brackets refer to values from the highest resolution shell