Silencing of phosphoglucose isomerase/autocrine motility factor ...
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Silencing of phosphoglucose isomerase/autocrine motility factor ...
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 37: 998-1004, 2016
Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration YANG LI, ZHENQING WEI, BIN DONG, ZHIGANG LIAN and YINGHUI XU Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China Received June 19, 2015; Accepted February 3, 2016 DOI: 10.3892/ijmm.2016.2500 Abstract. Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) is secreted by tumors and influences tumor growth and metastasis. In order to investigate the effects of silencing PGI/AMF on the migration and the sphere forming abilities of human glioblastoma U87 cells, as well as on the side population cells (SPCs), PGI/AMF was silenced using siRNA. Western blot analysis and RT-qPCR were used to assess the expression of PGI/AMF, Akt and SRY (sex determining region Y)-box 2 (SOX2). Wound healing, migration and tumorsphere formation assays were performed to assess invasion and metastatic potential. The proportion of SPCs was determined using Hoechst 33342 dye and flow cytometric analysis. PGI/AMF silencing inhibited the wound healing capacity and migration ability of U87 cells by 52.6 and 80.4%, respectively, compared with the scrambled siRNA (both P
