Step 1 Then ---→ Click search option ( from top right side) Real Time

Real Time PCR Primer design Step 1

Then --- Click search option ( from top right side)

Step 2

Then- come down and find (NCBI Reference Sequences (RefSeq)) option.

Step 4

Then  select option or click ( mRNA and Protein) and copy NM_001101.3)

Step 4

Go to

Integrated DNA Technologies - Home https://www.idtdna.com/

Then- Select Tools option  select qPCR assay Design select Real time PCR tool click

Step 5

Then - (Enter refseq accession numbers of interest) Then go to Step 4 enter NM_001101.3. Then  click Design Assays

Step 6

Then - select CDS option. Then Click Design Assays

Step 7

Then  wait for few seconds Then Primer page will display.

Step 8

Then Copy Forward and Reverse Primer Then Place order primer