SuperLooperâa prediction server for the modeling of loops in ...
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SuperLooperâa prediction server for the modeling of loops in ...
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Published online 8 May 2009
Nucleic Acids Research, 2009, Vol. 37, Web Server issue W571–W574 doi:10.1093/nar/gkp338
SuperLooper—a prediction server for the modeling of loops in globular and membrane proteins Peter W. Hildebrand1,*, Andrean Goede2, Raphael A. Bauer3, Bjoern Gruening3, Jochen Ismer1, Elke Michalsky3 and Robert Preissner3 1
Institute of Medical Physics and Biophysics, 2Institute of Biochemistry and 3Institute of Physiology, Charite´, University of Medicine, Berlin, Germany
Received February 20, 2009; Revised April 16, 2009; Accepted April 21, 2009
ABSTRACT SuperLooper provides the first online interface for the automatic, quick and interactive search and placement of loops in proteins (LIP). A database containing half a billion segments of water-soluble proteins with lengths up to 35 residues can be screened for candidate loops. A specified database containing 180 000 membrane loops in proteins (LIMP) can be searched, alternatively. Loop candidates are scored based on sequence criteria and the root mean square deviation (RMSD) of the stem atoms. Searching LIP, the average global RMSD of the respective top-ranked loops to the original loops is benchmarked to be