The alignment consists of transferrin sequences from organisms of various taxa. The disulphide bridges (Cys11-Cys49, Cys21-Cys45, Cys271-. Cys370 ...
Supplementary Figure 1 Multiple sequence alignment of transferrin. The alignment consists of transferrin sequences from organisms of various taxa. The disulphide bridges (Cys11-Cys49, Cys21-Cys45, Cys271Cys370, Cys320-Cys337, Cys334-Cys351 and Cys412-Cys426 in the first lobe; and Cys556-Cys593, Cys566-Cys584, Cys684-Cys788, Cys731-Cys754, Cys751-Cys769 and Cys837-Cys852), iron (Asp65, Tyr245, Tyr360, His434, Asp608, Tyr657, Tyr780 and His860) and anion (Thr273, Arg277, Ala279, Gly280, Thr686, Arg690, Ala692 and Gly693) binding residues can be referred using the number of amino acid position located at the top of this multiple sequence alignment.
Supplementary Table 1 Transferrin sequences obtained from Swiss-Prot and Ensembl database that included in the analysis. PROTEIN Transferrin invertebrate Otolith matrix protein-1
PROTEIN ID ENSCINP00000008273 Q0VIL3 Q9IBF7 ENSGMOP00000015079 ENSGACP00000013229 ENSTNIP00000020342 ENSTRUP00000039551 ENSORLP00000017320
Melanotransferrin
O97490 P08582 Q9R0R1 ENSOANP00000013922 ENSGALP00000032783 ENSACAP00000015439 ENSXETP00000041996 ENSGMOP00000020033 ENSTRUP00000046988 ENSTNIP00000008808 ENSGACP00000008232 ENSORLP00000014067
Novel transferrin
ENSXETP00000049884 ENSACAP00000019684 ENSGALP00000011051 ENSGMOP00000001223 ENSGACP00000001940 ENSTNIP00000000198 ENSTRUP00000038894 ENSORLP00000024770
ORGANISM Ciona intestinalis (Tunicate) Danio rerio1 (Zebrafish) Oncorhynchus mykiss (Rainbow trout) Gadus morhua1 (Cod fish) Gasterosteus aculeatus1 (Stickleback) Tetraodon nigroviridis1 (Spotted green pufferfish) Takifugu rubripes (Fugu) Oryzias latipes1 (Medaka) Oryctolagus cuniculus1 (Rabbit) Homo sapiens1 (Human) Mus musculus1 (Mouse) Ornithorhynchus anatinus (Platypus) Gallus gallus1 (Chicken) Anolis carolinensis1 (Anole lizard) Xenopus tropicalis1 (Western clawed frog) Gadus morhua2 (Cod fish) Takifugu rubripes2 (Fugu) Tetraodon nigroviridis2 (Spotted green pufferfish) Gasterosteus aculeatus2 (Stickleback) Oryzias latipes2 (Medaka) Xenopus tropicalis2 (Western clawed frog) Anolis carolinensis2 (Anole lizard) Gallus gallus2 (Chicken) Gadus morhua3 (Cod fish) Gasterosteus aculeatus3 (Stickleback) Tetraodon nigroviridis3 (Spotted green pufferfish) Takifugu rubripes3 (Fugu) Oryzias latipes3 (Medaka)
REFERENCE Flicek et al. 2011 Murayama et al. 2005 Murayama et al. 2004 Flicek et al. 2011
Kawamoto et al. 1998 Rose et al. 1996 Nakamasu et al. 1999 Flicek et al. 2011
Flicek et al. 2011
ENSDARP00000005435 Fish serotransferrin
JQ247193 ENSTRUP00000013200 P79819 ENSGACP00000017954 P80426 P80429 P79815 ENSGMOP00000011156 O93429
Saxiphilin
P31226
Amphibian serotransferrin Ovotransferrin-like
P20233 ENSACAP00000009871
Ovotransferrin
ENSGALP00000010383 P02789
Inhibitors carbonic anhydrase
Q9DBD0 Q29545
Mammals serotransferrin
P02787 P19134 Q921I1 Q29443 P27425
Mammals lactoferrin
P02788 P08071 P24627
Danio rerio2 (Zebrafish) Lates calcarifer (Asian seabass) Takifugu rubripes4 (Fugu) Oryzias latipes4 (Medaka) Gasterosteus aculeatus4 (Stickleback) Salmo salar1 (Atlantic salmon) Salmo salar2 (Atlantic salmon) Oncorhynchus kisutch (Coho salmon) Gadus morhua4 (Cod fish) Paralichthys olivaceus (Japanese flounder) Rana catesbeiana (American bullfrog) Xenopus laevis (African clawed frog) Anolis carolinensis3 (Anole lizard) Gallus gallus3 (Chicken) Gallus gallus4 (Chicken) Mus musculus3 (Mouse) Sus scrofa (Pig) Homo sapiens2 (Human) Oryctolagus cuniculus2 (Rabbit) Mus musculus2 (Mouse) Bos taurus1 (Bovine) Equus caballus (Horse) Homo sapiens3 (Human) Mus musculus4 (Mouse) Bos Taurus2 (Bovine)
Mohd-Padil et al. unpublished data Flicek et al. 2011 Mikawa et al. 1996 Flicek et al. 2011 Kvingedal et al. 1993 Kvingedal et al. 1993 Lee et al. unpublished data Flicek et al. 2011 Kim et al. unpublished data Morabito and Moczydlowski 1994 Moskaitis et al. 1990 Flicek et al. 2011 Flicek et al. 2011 Jeltsch and Chambon 1982 Church et al. 2009 Wuebbens et al. 1997 Jeffrey et al. 1998 Banfield et al. 1991 Chaudary and Skinner 1998 Retzer et al. 1996 Carpenter and Broad 1993 Rey et al. 1990 Pentecost and Teng 1987 Pierce et al. 1991
Supplementary Table 2 Characterization of the signal peptide, isoelectric point and phosphorylation sites of transferrin homologs using SignalP4.0, compute pI/Mw and NetPhos2 program, respectively. PROTEIN
PROTEIN ID
ORGANISM
SIGNAL PEPTIDE
pI
Transferrin invertebrate Otolith matrix protein-1
ENSCINP00000008273
Ciona intestinalis (Tunicate) Danio rerio1 (Zebrafish) Oncorhynchus mykiss (Rainbow trout) Gadus morhua1 (Cod fish) Gasterosteus aculeatus1 (Stickleback) Tetraodon nigroviridis1 (Spotted green pufferfish) Takifugu rubripes (Fugu) Oryzias latipes1 (Medaka) Oryctolagus cuniculus1 (Rabbit) Homo sapiens1 (Human) Mus musculus1 (Mouse) Ornithorhynchus anatinus (Platypus) Gallus gallus1 (Chicken) Anolis carolinensis1 (Anole lizard) Xenopus tropicalis1 (Western clawed frog) Gadus morhua2 (Cod fish)
Yes
8.22
Yes
4.96
8
5
3
Yes
5.05
8
4
6
No
5.08
8
3
3
Yes
5.30
7
2
4
Yes
5.69
11
2
5
Yes
5.76
7
3
5
Yes
5.17
9
2
5
Yes
5.81
16
19
9
Yes
5.66
19
10
7
Yes
5.75
23
6
11
Yes
5.43
28
8
14
Yes
5.97
11
9
7
No
6.36
23
4
5
Yes
6.36
21
6
11
No
6.09
17
7
16
Q0VIL3 Q9IBF7 ENSGMOP00000015079 ENSGACP00000013229 ENSTNIP00000020342 ENSTRUP00000039551 ENSORLP00000017320
Melanotransferrin
O97490 P08582 Q9R0R1 ENSOANP00000013922 ENSGALP00000032783 ENSACAP00000015439 ENSXETP00000041996 ENSGMOP00000020033
NUMBER OF PHOSPHORYLATIONS Ser Thr Tyr 16 9 8
ENSTRUP00000046988 ENSTNIP00000008808 ENSGACP00000008232 ENSORLP00000014067 Novel transferrin
ENSXETP00000049884 ENSACAP00000019684 ENSGALP00000011051 ENSGMOP00000001223 ENSGACP00000001940 ENSTNIP00000000198 ENSTRUP00000038894 ENSORLP00000024770 ENSDARP00000005435
Fish serotransferrin
JQ247193 ENSTRUP00000013200 P79819 ENSGACP00000017954 P80426 P80429
Takifugu rubripes2 (Fugu) Tetraodon nigroviridis2 (Spotted green pufferfish) Gasterosteus aculeatus2 (Stickleback) Oryzias latipes2 (Medaka) Xenopus tropicalis2 (Western clawed frog) Anolis carolinensis2 (Anole lizard) Gallus gallus2 (Chicken) Gadus morhua3 (Cod fish) Gasterosteus aculeatus3 (Stickleback) Tetraodon nigroviridis3 (Spotted green pufferfish) Takifugu rubripes3 (Fugu) Oryzias latipes3 (Medaka) Danio rerio2 (Zebrafish) Lates calcarifer (Asian seabass) Takifugu rubripes4 (Fugu) Oryzias latipes4 (Medaka) Gasterosteus aculeatus4 (Stickleback) Salmo salar1 (Atlantic salmon) Salmo salar2 (Atlantic salmon)
No
5.80
25
8
11
Yes
5.89
26
6
12
Yes
6.22
21
8
9
No
5.67
29
9
9
No
7.10
19
8
6
No
6.22
13
6
6
Yes
6.79
25
5
10
No
6.57
26
3
9
Yes
6.95
29
6
8
No
8.30
36
6
8
Yes
8.01
34
9
9
No
7.66
26
4
7
Yes
6.87
27
5
5
Yes
5.85
27
5
10
No
6.50
28
8
13
Yes
6.08
20
7
11
Yes
5.80
24
9
11
Yes
7.07
32
7
12
Yes
7.07
32
7
11
P79815 ENSGMOP00000011156 O93429 Saxiphilin
P31226
Amphibian serotransferrin Ovotransferrin-like
P20233 ENSACAP00000009871
Ovotransferrin
ENSGALP00000010383 P02789
Inhibitor carbonic anhydrase
Q9DBD0 Q29545
Mammals serotransferrin
P02787 P19134 Q921I1 Q29443 P27425
Mammals lactoferrin
P02788 P08071 P24627
Oncorhynchus kisutch (Coho salmon) Gadus morhua4 (Cod fish) Paralichthys olivaceus (Japanese flounder) Rana catesbeiana (American bullfrog) Xenopus laevis (African clawed frog) Anolis carolinensis3 (Anole lizard) Gallus gallus3 (Chicken) Gallus gallus4 (Chicken) Mus musculus3 (Mouse) Sus scrofa (Pig) Homo sapiens2 (Human) Oryctolagus cuniculus2 (Rabbit) Mus musculus2 (Mouse) Bos taurus1 (Bovine) Equus caballus (Horse) Homo sapiens3 (Human) Mus musculus4 (Mouse) Bos Taurus2 (Bovine)
Yes
6.15
24
6
11
No
6.29
20
0
9
Yes
6.06
18
11
7
Yes
8.03
29
10
13
Yes
7.73
19
9
9
Yes
6.92
26
10
11
Yes
6.85
20
8
7
Yes
6.85
18
10
8
Yes
7.00
20
8
8
Yes
5.88
19
8
6
Yes
6.81
18
13
8
Yes
6.43
14
9
9
Yes
6.94
16
8
10
Yes
6.75
22
12
8
Yes
6.83
21
11
7
Yes
8.50
26
6
9
Yes
8.90
23
13
7
Yes
8.69
18
12
8
