phosphatidylinositol-4-phosphate 5-kinase, type I, alpha ... coatomer protein complex, subunit alpha ... heat shock protein 90kDa beta (Grp94), member 1.
unilaterallary in PVN, paraventricular nucleus; LC, locus ceruleus; VMH, ventromedial hypothalamus; NTS, nucleus of the solitary tract; DMV, dorsal motor ...
EGFR-TKIs. Pre EGFR-TKI treatment. Types of EGFR mutation. No. Post EGFR-TKI. T790M (%). P. Our study. Gefitinib,. Erlotinib or. Afatinib. Exon 19 deletion.
Comparative pharmacokinetics of caffeine and its primary demethylated metabolites paraxanthine, theobromine and theophylline in man. Br J Clin. Pharmacol.
HWE rs11206510. 1. 55,268,627. C/T. C. 0.16. A/G. G. 0.19 0.987 0.438 rs17114036. 1. 56,735,409. A/G. G. 0.11. A/G. G. 0.11 0.924 0.229 rs599839. 1.
Incorporation efficiency of azido-modified nucleotide analogs showed ... and nucleotide composition of a sequencing library synthesized by the phosphoramidite.
... for hdPS deciles for the all-cause mortality model. Abbreviations: CI = confidence interval; HR = hazard ratio; hdPS = high-dimensional propensity score ...
The sample preparation process was carried out using the automated ... For ions with counts greater than 2 million, an accurate mass measurement ... Ions with less than two million counts require fragmentation ...... Carbohydrate. Glycolysis. TCA cyc
utilizing services provided âin the cloudâ by Amazon Web Services (Amazon Seattle, WA). A detailed schema for the survey and analytic data collection was ...
SME3358 ECL116 ârhaS λΦ(Phts-lacZ) recA::cat. This study. SME3359 SME3358 + pHG165lacI. This study. SME3632 SME3000 malP::lacIq zhc-511::Tn10 ...
Footnotes: (1) A generous gift from J. Kaufman, University of Cambridge, UK. (Kaufman et al. 1990, J. Immunol 133, 2258-2272). (2) Putative anti-CD11c.
GAL40. Corsica. 36. 42° 28.210'N 8° 38.950'E. PGP2016. Marseille. 10. 43° 11.190'N 5° 23.470'E. Table S2. Counts of SNP loci after each step of filtering. Step.
Construction of pBS-Z10 Construction of pBS-Z11 Construction of pBS-Z12 Construction of pBS-Z13 Construction of pBS-Z14 Construction of pBS-Z15 Construction of pBS-Z16 Construction of pBS-Z17 Construction of pBS-Z18 Construction of pBS-Z19 Construction of pBS-Z20 Construction of pBS-Z21 Construction of pBS-Z22
SUPPLEMENTARY FIGURES Figure S1. Nucleotide sequence of the DNA fragment introduced into pBluescript SK+ to give pBS-Z1. Cloning sites (KpnI and SacI) are underlined. Figure S2. Formation of a stable ternary complex between Cas1-Cas2, protospacer P1 and CRISPRcontaining plasmid pCOLA-Z0. Samples contained combinations of the indicated compounds: 400 nM of the DY782-labeled protospacer P1 (Fig. 1), 140 nM of Cas1 or of Cas1-Cas2 complex and 7.5 nM of either supercoiled pCOLA-Z0 or pCOLA-Duet1. All samples were analyzed by EMSA. The sample with Cas1-Cas2, protospacer P1 and plasmid pCOLA-Z0, was also treated with SDS at a final concentration of 0.5% (w/w) prior to electrophoresis (lane 1). The gel was scanned for fluorescence of DY782 (in green) and stained with ethidium bromide. The right side of the Figure is a zoom of the central part of the gel. Figure S3. Binding of Cas1-Cas2 to acceptor DNA, in the presence or absence of protospacer P1. Samples contained the indicated concentrations of Cas1-Cas2 (abscissa of the top graph) and 7.5 nM of either supercoiled pBS-Z0 or pBluescript SK+. If present, DY782-labeled protospacer P1 was included at a concentration in excess by a factor 1.2 over that of Cas1-Cas2. After 4 h incubation at 22°C, samples were analyzed by EMSA. Gels were stained with ethidium bromide and, when applicable, scanned for fluorescence of DY782. Bound and free plasmid concentrations were calculated from the gel experiments. Experiments were made in triplicate. Error bars in the top graph represent standard deviations (s. d.). The gels under the top graph display representative examples of the experiments. Shown are the parts of the gels where free and bound plasmids migrate. With pBSZ0 plus protospacer, DY782-labeled bound plasmid could be followed, not with pBluescript SK+. The weighted least-square fit of the data obtained with pBS-Z0 plus protospacer (thick blue line) yielded an apparent Kd value of 8.7 ± 1.3 nM and a plateau value of 6.4 ± 0.1 nM.
Figure S1. Sequence of the insert of plasmid pBS-Z1
