Supporting Information: Exploring the Structure and Phase Behavior of
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Supporting Information: Exploring the Structure and Phase Behavior of
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Supporting Information: Exploring the Structure and Phase Behavior of Plasma Membrane Vesicles under Extreme Environmental Conditions Janine Seeliger, Nelli Erwin, Christopher Rosin, Marie Kahse, Katrin Weise, and Roland Winter* Physical Chemistry I – Biophysical Chemistry, Department of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn-Str. 6, 44227 Dortmund, Germany
fresh 75-cm2 cell culture plates containing 25 mL GPMV medium composed of 90% MEM (without L-glutamine) supplemented with 10% FCS, 100 U/mL penicillin and 100 µg/mL streptomycin. If chemically induced blebbing was carried out with 25 mM formaldehyde and 3 mM DTT, almost all GPMVs showed lipid phase coexistence at RT (Table S1, row marked in green). In general, higher Tmisc was observed with increasing DTT concentrations (as reported previously [Levental, I., Grzybek, M., and Simons, K. (2011) Proc. Natl. Acad. Sci. U.S.A. 108, 11411−11416]) and poorer cell growth.
Additional Results Establishment of a cell growth and GPMV vesiculation protocol that generates GPMVs with lipid phase coexistence at room temperature.
Table S1. Cell growth and GPMV blebbing conditions including 25 mM formaldehyde and 2-5 mM DTT.
