www.sciencemag.org/cgi/content/full/332/6025/103/DC1
Supporting Online Material for Perception of UV-B by the Arabidopsis UVR8 Protein Luca Rizzini, Jean-Jacques Favory, Catherine Cloix, Davide Faggionato, Andrew O’Hara,2 Eirini Kaiserli, Ralf Baumeister, Eberhard Schäfer, Ferenc Nagy, Gareth I. Jenkins, Roman Ulm* *To whom correspondence should be addressed. E-mail:
[email protected] Published 1 April 2011, Science 332, 103 (2011) DOI: 10.1126/science.1200660 This PDF file includes: Materials and Methods Figs. S1 to S7 References
1
SUPPORTING ONLINE MATERIAL
MATERIALS AND METHODS
Plant material and growth conditions. cop1-4 and uvr8-6 are in the Columbia ecotype (Col) (1, 2). The cop1-4/Pro35S:YFP-COP1, cop1-4 uvr8-6/Pro35S:YFP-COP1 and Col/ Pro35S:CFP-UVR8 lines were described before (2, 3).
UV-B irradiation. Plants were grown and irradiated exactly as described previously (2, 4). Irradiation of cell-free protein extracts was carried out on ice. Longer-term irradiations (>1h) in experiments with HEK293T cell extracts and yeast two-hybrid analysis used narrowband UV-B tubes (Philips TL20W/01RS, 1.5 mol m-2 s-1) (see spectrum in fig. S7). Short-term irradiations (
