Table S1. Bacterial strains and plasmids used in this ...
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Table S1. Bacterial strains and plasmids used in this study. Strain/plasmid. Descriptiona. Source. S. pneumoniae. D39. Serotype 2 strain, cps 2. PcopY-lacZ.
SUPPLEMENTARY MATERIAL
Table S1. Bacterial strains and plasmids used in this study. Strain/plasmid
This study This study This study This study This study This study This study This study
ΔcupA copA
D39 spd0634-5::SpecR
This study
copA-
D39 spd0635::SpecR
This study
E. coli EC1000 Plasmids pPP2
pORI280 pSS1 pSS2 pSS3 pSS4 pSS5 pSS6
KmR; MC1000 derivative carrying a single copy Laboratory of the pWV1 repA gene in glgB collection AmpR TetR; promoter-less lacZ. For replacement of bgaA with promoter lacZ fusion. Derivative of pTP1 ErmR; ori+ repA-; deletion derivative of pWV01; constitutive lacZ expression from P32 promoter pORI280 copY-stop, containing two stop mutations after 6 base of coding sequence pORI280 cupA-stop, containing two stop mutations after 21 base of coding sequence pPP2 PcopY-lacZ, containing the whole promoter pPP2 PcopY-wt-lacZ, containing cop motif 1 and 2 pPP2 PcopY-mut-lacZ containing cop motif 1 only pPP2 PcopY+copY-lacZ
1
Halfmann et al., 2007 Leenhouts et al., 1991 This study This study This study This study This study This study
