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World Journal of Microbiology and Biotechnology
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Identification of non-TAL effectors in Xanthomonas oryzae pv. oryzae Chinese
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strain 13751 and analysis of their role in the bacterial virulence
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Shuai Zhao · Wei-Lan Mo · Fan Wu · Wei Tang · Ji-Liang Tang · Boris Szurek · Valérie
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Verdier ·Ralf Koebnik ·Jia-Xun Feng
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S. Zhao ·W.-L. Mo ·F. Wu ·W. Tang ·J.-L. Tang ·J.-X. Feng ()
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State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Key
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Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life
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Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China
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E-mail:
[email protected]
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S. Zhao ·B. Szurek ·V. Verdier ·R. Koebnik
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Institut de Recherche pour le Développement, UMR RPB IRD-CIRAD-UM2, 911 Avenue
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Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
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Online Resource 1 Validation of mutants of genes encoding T3SS secreted
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non-TAL effectors in Xoo Chinese strain 13751. The confirmation of the mutation of
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the nine identified T3SS non-TAL effector genes (xopW13751, xopF13751, xopAE13751,
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xopN13751, xopY13751, xopAA13751, xopAB13751, xopP13751 and xopR31751) by PCR was
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shown in a-i. The deletion mutants of xopW13751, xopF13751, xopN13751, xopAB13751,
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xopP13751 and xopR31751 and the insertional mutants of xopAE13751, xopY13751,
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xopAA13751 were confirmed using the corresponding primer pairs given in Table S4. M:
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1kb DNA ladder, a (5), b (8), c (4), d (6), e (4), f (3), g (1-2), h (6) and i (7) represent
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Xoo wild-type strain 13751, others shown were mutants for the non-TAL effector
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genes xopW13751
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xopY13751 (e1-3), xopAA13751 (f1-2), xopAB13751 (g3-5), xopP13751 (h1-5) and xopR31751
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(i1-6).
(a1-4), xopF13751 (b1-7), xopAE13751 (c1-3), xopN13751 (d1-5),
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