Table S1 N-terminal sequence and predicted processing of ... - PLOS

Table S1 N-terminal sequence and predicted processing of yeast signal sequences

Protein ADP1 AGA1 AGA2 AIM6 ANS1 APE3 ASP3-1 ASP3-2 ASP3-3 ASP3-4 ATG15 ATG27 ATH1 AWA1 AXL2 BAR1 BGL2 BIG1 CCW12 CCW14 CDA1 CDA2 CIS3 CNE1 CPR4 CPR5 CRH1 CRR1 CSG2 CTS1 CTS2 CWH43 CWP1 CWP2 CYP2 DAN1 DAN2 DAN3 DAN4 DCW1 DDR2 DFG5 DIA3 DSE2 DSE4 ECM14 ECM33 EGT2

N-terminal residues MG MT MQ MLG MK MHF MR MR MR MR MLG MV MK MFN MT MS MR MQ MQ MR MK MR MQ MK MWL MK MK MR MS MS MV MLI MK MQ MK MS MV MV MV MLV MK MI MV MK MQ MLH MQ MN

Predicted Met cleavage Cleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Cleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved

Predicted Acetylation* Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated *

EMC1 EMP24 EMP46 EMP47 EMP70 EPS1 ERJ5 ERO1 ERP1 ERP2 ERP3 ERP4 ERP5 ERP6 ERV25 EUG1 EXG1 EXG2 FET3 FET5 FIG2 FIT1 FIT2 FIT3 FLC1 FLC2 FLC3 FLO1 FLO10 FLO11 FLO5 FLO9 FMN1 FPR2 FRE1 FRE2 FRE3 FRE4 FRE5 FRE6 GAB1 GAS1 GAS2 GAS3 GAS4 GAS5 GPI16 GPI17 GPI8 GRX6 GTB1 HKR1 HOR7

MK MAS MT MMM MI MK MN MR MLL MI MS MR MR MLS MQ MQ MLS MP MT MLF MN MK MK MK MQ MI MR MT MP MQ MT MS MFT MMF MV MHW MY MLL MLF MHR MD MLF MN MQ MMV MLL MI MS MR MI MV MV MK

Uncleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Cleaved Uncleaved Cleaved Cleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Cleaved Cleaved Uncleaved

Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated * Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated * Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Acetylated Non-acetylated Acetylated * Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated

HPF1 HRD3 HSP150 IRC22 IRE1 JEM1 KAR2 KAR5 KEX1 KEX2 KNH1 KRE1 KRE27 KRE5 KRE9 LHS1 LRC1 MEL1 MEL2 MEL5 MEL6 MFALPHA MFALPHA2 MID1 MID2 MKC7 MNL1 MNN5 MPD1 MPD2 MRL1 MSB2 MTL1 MUC1 NCP1 NCR1 NPC2 NVJ1 OST1 OST3 OST6 PAU10 PAU13 PAU15 PDI1 PEP1 PEP4 PER1 PGA1 PGU1 PHO11 PHO12 PHO3

MFN MI MQ MR MR MI MFF MFE MFY MK MLI MMR MS MR MR MR MLG MFA MFA MFA MFA MR MK MI MLS MK MV MLI MLF MK MLK MQ MAS MQ MP MN MT MT MR MN MK MV MV MV MK MI MFS MR MV MI MLK MLK MFK

Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Cleaved Uncleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Cleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved

Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Acetylated Acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Acetylated * Acetylated Acetylated Non-acetylated Acetylated * Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Acetylated Acetylated

PHO5 PIR1 PIR3 PLB1 PLB2 PLB3 PRB1 PRC1 PRY1 PRY2 PRY3 PST1 PST2 PTM1 RAX2 RNY1 ROT1 ROT2 RRT12 SAG1 SCJ1 SCW10 SCW11 SCW4 SED1 SHE10 SIA1 SIL1 SIM1 SLG1 SLP1 SOP4 SPI1 SPO19 SPR1 SPS100 SPS22 SRL1 SSP120 STA1 STA2 SUC1 SUC2 SUC4 SUN4 SVS1 SWP1 TED1 THI22 TIP1 TIR1 TIR2 TIR3

MFK MQ MQ MK MQ MI MK MK MK MK MLE MQ MP MR MFV MLL MWS MV MK MFT MI MR MI MR MK MG MR MV MK MR MAN MFS MLS MK MV MK MN MLQ MR MV MQ ML MLL ML MK MI MQ MLR MV MS MAY MAY MS

Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Cleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Cleaved Cleaved Cleaved Cleaved

Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated * Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Acetylated

TIR4 TMN2 TMN3 TOS1 UIP5 UTR2 VOA1 VTH1 WBP1 WSC2 WSC3 WSC4 YBL008W-A YBR013C YBR200W-A YCL012C YCL048W-A YCL049C YCR012C YDR053W YDR134C YDR246W-A YDR262W YDR366C YDR415C YDR524C-B YER067W YFL051C YFR012W-A YGP1 YGR079W YHC3 YHL017W YHL042W YHR138C YHR214W YIL156W-B YIL169C YJL052C YJL160c YJL171C YJR120W YKL018C-A YLR001C YLR040c YLR042C YLR104W YLR194C YLR406C-A YLR413W YLR414C YMR247W-A YMR272W-B

MAY MK MR MLQ MS MAI MV MAL MR MHL ME MQ MK MI MLL MK MQ MFS MK MR MQ MR MI MV MR MQ MT MS MLP MK MS MS MD MK MK MFN MT MFN MHL MHY MLQ MR MLG MN MI MK MS MK MI MN MR MAH MR

Cleaved Uncleaved Uncleaved Uncleaved Cleaved Cleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Cleaved Cleaved Uncleaved Uncleaved Cleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved

Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Acetylated Acetylated Acetylated Non-acetylated Non-acetylated Acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Acetylated * Non-acetylated Non-acetylated Acetylated Non-acetylated Non-acetylated Acetylated * Non-acetylated Non-acetylated Non-acetylated

YMR315W-A YNL019C YNL024C-A YNL033W YNL217W YOR008C-A YOR214C YOR365C YOR389W YOS9 YPS1 YPS3 YPS5 YPS6 YPS7 YSP3 ZPS1

MT MLY MS MLY ME MWR MLG MLP MR MQ MK MK MQ MQ MT MK MK

Cleaved Uncleaved Cleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Uncleaved Cleaved Uncleaved Uncleaved

Non-Acetylated Non-acetylated Acetylated Non-acetylated Acetylated Acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated Non-acetylated

* MN acetylation is predicted in ony 55% of cases