Custom TaqMan gene expression assay for retroviral ... Bisulfite methylation analysis of Oct4 distal enhancer ... Taqman assay for ChIP analysis of gene.
Bam. GTGGATCCCCCTCCTTACTATAGAAAATTCG. Primers used for the amplification of promoters of C. thermocellum DSM 1313 cellulosomal genes during ...
[7, 8, 9]. C. burnetii com1. CBCOS. CBCOE. GCTGTTTCTGCCGAACGTAT ... c The positive control for the B. microti (q)PCR is a DNA lysate of a spleen from a ... Maanen C, Butler CM, Földvári G, Szekeres S, van Duijvendijk G, Tack W, Rijks ...
CGA ATT GTG GAT AAG CTT TCA TCT GC. pG+host4 flanking multiple cloning site. M14. TTG TAA AAC GAC GGC CAG TGA. RedR. ACA GGA AAC AGC TAT ...
Table S3. Primers used in this work. Primer. Sequence (5`-3`)a. Locationb. PCR product size (bp). Use. Reference or source. ACrnaF.
Primer Number. Sequence. Information. CGCGCGCCAAGCTTGATGTCTTTGTTCA Clone LapD for B2H Fwd. AACAGCTGTTGATCGCTATC.
51.25 kDa recombinant OsPAP21b protein (2 µg) purified by Ni2+-affinity chromatography. (d) Western blot analysis of purified 6XHIS-OsPAP21b with anti-.
pNE1 PermAM-FLAG-RitR (C128A). Maule et.al. 2015. pE-SUMO. N-terminal His6-SUMO fusion protein expression vector. Life Sensors Inc. pE-SUMO-RitRWT.
Sep 17, 2015 - sequencing. Primer Name Primer. Source. Primer sequence (5' to 3'). PE Adapter. Illumina^. ACACTCTTTCCCTACACGACGCTCTTCCGATCT.
Oct 8, 2014 - DONOR VECTOR. USED. pAA11. attL4::wee-1.3 short promoter::attR1. oAKA2 and oAKA3. pDONR(P4-P1r). pAA13. attR2::wee-1.3 3'UTR:: ...
GAT CAA ATT TAT GGT TTG ATC GCT ATT CG. Arm 2 of relAÎSYN mutagenesis. RelA::D264G-D. GGC CAC ATA GCC CTG CTC. Arm 2 of relAÎSYN ...
Cytokinin (BAP) bud induction assay. WT protonemal tissue (a) and (c);. âdek1 protonemal tissue (b) and (d). Protonemal tissue in (c) and (d) were treated for 48.
at 30°C, washed twice with dH2O, diluted to 0.01 OD600, (except for the tpk1/tpk1 ... TPK2 complemented strain rescues hyphal growl and biofilm formation.
Sequence (5' to 3'). Primers for gene cloning. ccpAF. CTAGCTAGCGGCTATTTTTATATGGAAAAACAAAC. ccpAR. CGAACTTAATCAGCAGACTTGG. Primers for ...
Primers used in this study to measure gene expression level by qPCR. Gene Symbol. Primer sequence. RAD18. Forward: GGGAAGCATCACATAAAAACG.
TABLE S1 Real time PCR primers. Gene. Primer sequence. ACO2. AATGGATGTACTCGTTGGGC. ACO2. ACAGCCTACTGGTGACTCGG. ACO2.
Qrr2 was present at 1.4 µM in each case. On the right, the upper panels show a ribbon representation of Qrr2, predicted by iFoldRNA (26,27), superimposed with.
B. Digital panning of a diverse donor-17 scFv library against a clade-C V1V2-ferritin nanoparticle (negative control). H. 28,145. 26,448. 16.5%. Pan0. 171,308.
Table S1 Primers used in our experiments Primers Primer ... - mBio
Construction of ligase-defective strain Amplifiing PilE Construction of PglLmut
Primers below were applied to create mutation of amino acids by using nested-PCR SacI-U was the upstream primer for the following amplifications. 5573-D1(5569D1)
