Table S3. List of the primers used in the study.

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Table S3. List of the primers used in the study.

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Table S3. List of the primers used in the study. Primers used to produce U6shSLY vectors. Primer name. Sequence sh136 reverse primer.

Table S3. List of the primers used in the study. Primers used to produce U6shSLY vectors Primer name

Sequence

sh136 reverse primer

AAAAAAAGGACGATGACATAAAAGTTTCCCATCTGTGGCTTTACAGAAACTTTTATGTCATCGTCCAAACAAGGCTTTTCTCCAAGGG

sh367 reverse primer

AAAAAAGGATAAATCTGGAGATGACACCCATCTGTGGCTTTACAGTGTCATCTCCAGATTTATCCAAACAAGGCTTTTCTCCAAGGG

shIRR reverse primer

AAAAAACACTACCGTTGTTATAGGTGCCCATCTGTGGCTTTACAGCACCTATAACAACGGTAGTGAAACAAGGCTTTTCTCCAAGGG

U6 Forward primer

ATCCTCTTAAGTCGACGCCGCCATCTCTAG

Primer name

Sequence

β-actin-F

GGCACCACACCTTCTACAATG

β-actin-R

GTGGTGGTGAAGCTGTAGCC

Slx-F

TTCAGATGAAGAAGAAGAGCAGG

Slx-R

TCCATATCAAACTTCTGCTCACAC

Slx-like-F

TTGGAGGACGCTCATTCTG

Slx-like-R

ACGACTTGTTGTTGATCATCTCC

H2AL1-F

CCAGAGAGGTGAGCTTCCTC

H2AL1-R

CAGGCATCATCAGTTGTCATC

Actrt1-F

CTCAAAAATGGTCTGCAACAGC

Actrt1-R

TCTTGATAGGGGTTCCCTCAAA

1700008I05Rik-F

AAAGCCAATTCGTGGAGACAAT

1700008I05Rik-R

TGGGAGAGATGCAGAATATCCA

Ssty1-F

AGAAGGATCCAGCTCTCTATGCT

Ssty1-R

CCAGTTACCAATCAACACATCAC

Ssty2-F

CAGGTGCCATTCTTACAGGACTAT

Ssty2-R

ACCCAGGAACCTATTAAGAAGTCAT

Asty-F

GRGGAGTAGAACTCATCATC

Real time PCR primers* Gene name β-actin

Reference [58]

Slx (aka Xmr )

[6]

Slx-like (aka AK015913 )

[6]

H2AL1 (aka AK005922 )

[6]

Actrt1*

1700008I05Rik*

Ssty1

[6]

Ssty2

[6]

Asty

[6]

Asty-R

CAGGAGATGACTAACATAGCA

Orly-S1.f2

TCCCAGTGGTGTATGAAAGG

Orly-N1.r

GCCATTGTCTGATGAAAGTACC

Zfy2-F

CTTAATTCCAGACATTTTAACTTCCA

Zfy2-R

ATCACTTGTTCAAAATGTCCTACATT

Ubb-F

GAGGGGTGGCTATTAATTATTCG

Ubb-R

CTAAACTTAAATTGGGGCAAGTG

Acrv1-F

TGAGTACACCACTTCCAAGCA

Acrv1-R

AAGCACATGTGTGGCAATTT

Protamine1-F

ACAAAATTCCACCTGCTCACA

Protamine1-R

GTTTTTCATCGGaCGGTGGC

Hsf2-F

ACCCACACCAACGAGTTCAT

Hsf2-R

TGCTCATCCAAGACCAGAAA

Oas1b-F

ATCTCATCAGTGGGCGTACC

Oas1b-R

CCAGGCATAGACTGTGAGCA

Global Sly F

CATTTATAAGACGCTTCACATAAAG

Global Sly R1

TCCTCCATGATGGCTCTTTC

Global Sly R2

ATTCTCCATGATGGCTCTTTC

Probe name

Sequence

mir-t3 probe

TTACATGAGCTCAAAATCATAC

mir-t25 probe

AGTAACTGCTACTTCTCTCAGCA

U6snRNA probe

TTCACGAATTTGCGTGTCATCCTTGCG

Orly

[16]

Zfy2*

Ubb

[6]

Acrv1 (aka SP-10 )

[59]

Protamine 1

[60]

Hsf2

[59]

Oas1b*

Sly (global PCR)*

Additional probes for small Northern blot

* newly designed primer pairs for Real time PCR were designed to span an intron boundary and the identity of the amplified product was confirmed by sequencing.

[61]