Page 1 Table S1: List of the primers used in this study. Primer ...

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Table S1 List of primers used in the study

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SUPPLEMENT Table S1. Primers used in protocol

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Page 1 Table S1: List of the primers used in this study. Primer ...

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CRT-PCR Locus 18638. CRT-PCR_Pal-18638-01 AATGGTAAAATAGTCCGAAGGTCCAC. CRT-PCR_Pal-18638-02 CGAACCCGCGACCCTTCAG.

Table S1: List of the primers used in this study.

Primer Sequence (5'à3') LAM-PCR (TRE5-A ORF1-specific) Rep-234_bio Biotin-CAAAATAAATAACTGTTGAAATTG Rep-235 CTTGAACATCTTCACCATCC 454B-6N GCCTTGCCAGCCCGCTCAGNNNNNN bsr PCR screening for TRE5-A integrations at tRNA gene families (LAM-PCR library) AlaAGC CCCTTTACCTCTCGCATGCTAAGC AlaUGC GATCCCTTTACCTTATGGAACTCTG ArgACG CTACCGGTTCGTAGCCGG ArgCCU GCATCTTATTCGTTAGGACCG ArgUCU CCTATTGATTCGAAGTCAATCGC AsnGUU GGACCTAGCGGTTAACAGCCGCTC AspGUC CCTTTCGCGTGACAGGCGAAAATC CysGCA GACCTAACGATTTGCAGTCGCTTG GlnUUG CAGATTACAGGATTCAAAGTCCTG GluUUC CGAGGTACCAGTGTGAAAGACTAG GlyUCC CGGTTCAACTGCTTGGAAGGCAGC HisGUG GGTCAACAGAGCCACAATCTGATG IleAAU CGATGCAGGGATCGAACCTGC IleGAU GCTTATCAAACGAATACTCTAACC IleUAU CTCGCGACCAAAGCATTGC LeuUAA GAACCGAATGGATGAGATCTTAAG LeuUAG CCTAAATCTATTGTCTTAACCAC LysUUU CCGACCACAAGGTTAAAAGCCTTG MetCAU GGACCTTAGGATTATGAGACC PheGAA CGACCTTAAGATCTTCAGTCTCAC ProUGG CCTATAATCTTTAGTTCCCAAAAC SerAGA CGAAGGGATCTGATTTCTAGTC SerCGA CTTTTATATGATTCGAGTCCAACTC SerGCU GAGAACGCCTTAGCAGGGC SerUGA GCGCCTCCTAGGAGATTTGATTTC ThrAGU GACCTCTTCCTTACTAGGGAAGCG ThrCGU GTATAATTTTATACGAGAGAAATGTCTTAC ThrUGU GACCCTCTGTTATCGCTTTAGCAC TrpCCA CTGGACCAACTGCTAACTTCTAAC TyrGUA CAACCGATTGATCTCAATTATTAC ValAAC ACGACCTAATCCGTGTTAAGGATT ValCAC GACCTAACCCGTGTGAAGGGC ValUAC GAGACCTGTTGCGTGTAAAGCAAC bsr PCR screening for TRE5-A integrations at B-box loci on the rDNA palindrome Pal-2656-rev GAATTTAGAGATTAAAACTTTTTGGAG Pal-6106-rev CTTTAGTGCAGATCTTGATGGTAGTAG Pal-6483-rev GGGTATCATTTTAATTCACTTAATTGG Pal-7762-rev CACCTTCTACGACTCGAAATTCCAAAC Pal-9175-rev TGTGATGCCCTTAGATACCTTGGGCCG Pal-10430-rev GGTATTGATATTTATTGTCACTACTTC

Pal-13997-rev CCCACTTTTTTGTACCCTTTGTAGGTC Pal-15009-rev TTCCTAAATGAGCAATTTGACATTCCC Pal-15156-rev AACAACTTTTTTAAGGTATAGTTACTC Pal-18638-rev AAATGGTAAAATAGTCCGAAGGTCCAC Pal-25439-rev GTGGATGCTCCAGGGAGTTATCTATTC Pal-26963-rev GTAGATGCCAATTGGAGTAATATATTC Pal-27726-rev TGAGGTAACAGTAGATTCTCCATTGAG Pal-29052-rev ATTAGGTCATAGGTTTCTGGATCGATC Pal-29059-rev CAACGGGGAATGGAAACGTCTTGAAAG Pal-29115-rev TCTAAGTTAGATTTTTACTAGTAATTC Pal-31700-rev TACCATTAGATAGCCCTATTTCTCTAG Pal-33496-rev GGAGTTGTCTATCCAACAAGGGTAATC bsr PCR screening for TRE5-A integrations at the r5S gene on the rDNA palindrome 5S-rDNA-03 CCAACCAGAGTACTTGGAAGGC PCR screening for endogenous TRE5-A integrations at the r5S gene on the rDNA palindrome 5S-rDNA-04 GTATACAGCACCCTATGTTCCCCTG Rep-190 CCATAGGTTGGGCTGAAACT 5S-rDNA-03 CCAACCAGAGTACTTGGAAGGC Rep-109Q GTTAGATTGTCTAGTTCAATGATAGTGTC PCR screening for endogenous TRE5-A integrations at B-box loci on the rDNA palindrome Pal-06 GTAATGGAGTAAGCTATCTAATGATAC Rep-190 CCATAGGTTGGGCTGAAACT Pal-18638-rev AAATGGTAAAATAGTCCGAAGGTCCAC Rep-157Q CTTCCGTTGTTAGTCTCTTTGTTTCGCC cRT-PCR Locus 18638 cRT-PCR_Pal-18638-01 AATGGTAAAATAGTCCGAAGGTCCAC cRT-PCR_Pal-18638-02 CGAACCCGCGACCCTTCAG cRT-PCR_Pal-18638-03 GTATCATTAGATAGCTTACTCCATTAC cRT-PCR_Pal-18638-04 GTTGCTTTGGCTTAGTTGGTTAAGCG cRT-PCR_Pal-18638-05 GCATACTATTATATATTTTT GGCC cRT-PCR Locus 25439 cRT-PCR_Pal-25439-01 CTATTACATAATAATGCAACC cRT-PCR_Pal-25439-02 GAGAATCGAACCCTGGAC cRT-PCR_Pal-25439-03 CTTTATATTGTCTAATGACCTCC cRT-PCR_Pal-25439-04 GATGTCTAAAAATAAACCGAG cRT-PCR_Pal-25439-05 TGCTGACTAATCTACCAC cRT-PCR Locus 26963 cRT-PCR_Pal-26963-01 GACCCCTCTATAATGCAACC cRT-PCR_Pal-26963-02 AAAATCGAGAATCGAACCCG cRT-PCR_Pal-26963-03 CAATTGGCATCTACCATTACC cRT-PCR_Pal-26963-04 GGTGGATTAGTCATCATATGTC cRT-PCR_Pal-26963-05 CTTTATGTGGTCTAATGACCTTC RT-PCR palindrome locus 18638/22168 Pal-RT_04 GTTGCTTTGGCTTAGTTGGTTAAGCG Pal-RT_05 AATGGTAAAATAGTCCGAAGGTCCAC