(V) 235 158 20 Acrolein-DNPH

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Appendix Figure S2. (A) Effect of acrolein on GPX5 and GST1 protein levels of mixotrophic. Chlamydomonas reinhardtii treated with 0 and 120 ppm acrolein for ...
Q1 Mass Q3 Mass Time (Da) (Da) (msec) 235 158 20 307 152 20 251 204 20 277 181 20 263 46 20 331 286 20

ID Acrolein-DNPH 2-Ethylhexanal-DNPH (IS) Malondialdehyde-DNPH trans-2-Hexenal-DNPH trans-2-Pentenal-DNPH b-Cyclocitral-DNPH

DP (V) -55 -90 -60 -70 -55 -80

CE (V) -20 -26 -22 -28 -44 -32

CXP (V) -10 -13 -15 -11 -5 -9

A

Acrolein (ppm) 0

120

4 8

4 8

(h)

GST1

24kD

GPX5

16kD

B

70 40 35 25 15 10 (kD)

Appendix Figure S2. (A) Effect of acrolein on GPX5 and GST1 protein levels of mixotrophic Chlamydomonas reinhardtii treated with 0 and 120 ppm acrolein for 4 and 8 h. (B) Coomassiestained identically loaded parallel ran gel is shown for loading control.

α-Tocopherol (% of control)

a

100

a

a a

75 50

b

25 0 0

600 1200 1800 2400 Acrolein treatment (ppm)

Appendix Figure S3. Effect of 4 h acrolein treatments on α-tocopherol contents of mixotrophic

Chlamydomonas reinhardtii. Different letters indicate significant difference at P