Validation of a network-based strategy for the ...
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Validation of a network-based strategy for the ...
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www.impactjournals.com/oncotarget/
Oncotarget, Supplementary Materials 2016
Validation of a network-based strategy for the optimization of combinatorial target selection in breast cancer therapy: siRNA knockdown of network targets in MDA-MB-231 cells as an in vitro model for inhibition of tumor development SUPPLEMENTARY FIGURES
Supplementary Figure S1: The knockdown of individual components of the network target has no detectable effect on MDA-MB-231 cells growth, survival and death. MDA-MB-231 cells were transfected with scrambled siRNA or individual
siRNA to HSP90AB1, CSNK2B, TK1, YWHAB and VIM. Cells were processed for growth A. and MTT survival B. assays as described in Materials and Methods. Percent dead cell in transfectants was determined by propidium iodide staining and cytometry C-H. The percentages of cell death (sub-G1) are indicated as “M1”.
www.impactjournals.com/oncotarget/
Oncotarget, Supplementary Materials 2016
Supplementary Figure S2: The knockdown of individual components of the network target has no detectable effect on MDA-MB-231 cells foci formation, growth in soft agar, migration or invasion. MDA-MB-231 cells were transfected with
scrambled siRNA or individual siRNA to HSP90AB1, CSNK2B, TK1, YWHAB and VIM. Cultures were processed for foci formation A,B., soft agar growth C., migration D. and invasion E. as described in Materials and Methods. Histograms represent the average number of colonies, migrated/invaded cells ± SD of 3 independent experiments.
