A Microfluidic Device for Temporally Controlled Gene Expression and
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Background. Imaging single cells with fluorescent markers over multiple cell cycles is a powerful tool for unraveling the mechanism and dynamics of the cell cycle. Over the past ten years, microfluidic techniques in cell biology have emerged that allow for good control of growth environment. Yet the control and quantification of transient gene expression in unperturbed dividing cells has received less attention. Methodology/Principal Findings. Here, we describe a microfluidic flow cell to grow Saccharomyces Cerevisiae for more than 8 generations (
