Additional file 3 Figure S1. Validation of differentially ...
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Additional File 3: Differentially expressed genes (3.25 fold) in double HAT mutants. Down-regulated. âgcn5âelp3. âgcn5âmst2. âmst2âelp3. Gene. Log2.
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Additional file 3. 0. 60. 120. 180. Ica1 c-fos. Ifrd1. Btg2. Uhrf. Hox2a. Ube2. F o ld C h ang e. 0. 1. 2. 3. 4. 5. Ica1 c-fos. Ifrd1. Btg2. Uhrf. Hoxa2 Ube2b. Fo ld. Ch a.
Additional file 3
Fold Change
5
cDNA microarray
4 3 2 1 0 Ica1
Fold Change
20
Ifrd1
Btg2
Uhrf
Hoxa2
Ube2b
Illumina BeadChips
18 16 14 12 10 8 6 4 2 0
ND Ica1
180
Fold Change
c-fos
c-fos
Ifrd1
ND Btg2
Uhrf
Hoxa2
Ube2b
qRT-PCR assay
120
60
ND
0 Ica1
c-fos
Ifrd1
Btg2
Uhrf
Hox2a
Ube2
Figure S1. Validation of differentially expressed genes by SYBR greenbased quantitative real-time PCR. The figure shows fold changes from a few selected genes on the microarry platforms and relative gene expression data from qRT-PCR assays (lower panel). qRT-PCR was performed in triplicates. Relative gene expressions (fold changes) were calculated by 2-ΔΔCt [1] and mean ± SD values are shown. Red bars indicate genes up-regulated in AR42J cells compared to NRK52E cells. Green bars indicate genes relatively highest expressed in the NRK52E cell line. ND: Not Detectable. More detailed information is given in Additional file 4. PCR primers and qRT-PCR protocol are given in Additional file 5. 1.
Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25(4):402-408.
