Supplementary
Identification and Expression Profiling of the Auxin Response Factors in Capsicum annuum L. under Abiotic Stress and Hormone Treatments Chenliang Yu 1,*, Yihua Zhan 2, Xuping Feng 3, Zong-An Huang 4 and Chendong Sun 2 1
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Vegetable Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China;
[email protected] (Y.Z.);
[email protected] (C.S.) Key Laboratory of Spectroscopy, Ministry of Agriculture, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China;
[email protected] Institute of Vegetable Sciences, Wenzhou Academy of Agricultural Sciences, Wenzhou 325014, China;
[email protected]
* Correspondence:
[email protected]; Tel.: +86-18768141629
Figure S1. Sequence identity matrix of pepper ARF proteins. Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/) program were employed to examine sequence identity of 22 pepper ARF proteins.
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Figure S2: Phylogenetic relationships of CaARF proteins (a) and Gene structure analysis of CaARF genes (b).
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Figure S3. Conserved motif analysis of CaARF proteins. a. Conserved motif of CaARF proteins predicted by MEME tool. b. Conserved motif sequences of CaARF proteins.
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a
CaARF5
5’--GGGCATGCAGGGAGCCAGGCA---3’
CaARF15
5’--TGGCATGCAGGGAGCCAGGCA---3’
CaARF8
5’--AGGCATACAGGGAGCCAGGCA---3’
CaARF17
5’--AGGCATACAGGGAGCCAGGCA---3’ :
AtmiRNA160
:
3’--ACCGUAUGUCCCUCGGUCCGU---5’
b CaARF22
5’--AAGATCAGGCTGGCAGCTTGT---3’
CaARF13
5’--GAGATCAGGCTGGCAGCTTGT---3’
CaARF21
5’--GAGATCAGGCTGGCAGCTTGT---3’ :
AtmiRNA167
: :
:
3’--AUCUAGUACGACCGUCGAACU---5’
Figure S4. Sequences of AtmiRNA160 (a) and target AtmiRNA167 (b) sites on CaARF mRNAs. The Watson-Crick base pairings to the AtmiRNA160 and AtmiRNA167 sequence are shown.
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Table S1. List of primers used in the present study. Gene name
CaARF7 qRT CaARF8 qRT CaARF9 qRT CaARF10 qRT CaARF11 qRT CaARF12 qRT CaARF13 qRT
Forward primer(5'to3') ACAAACCGAGATATATGGGCA CT TCTCTGCACAGTGATCAGTCA TACGGAGCTTTGGCGTTCAT AGATGGCCTGGTTCCAAGTG CCCTTCATTCTCTGCCGTGT AACTTCTCACATTGGCAGTGCT C TTGGGCCAATCCAGGATCAC TCTGGCCGTTTAGGCACATT AGCATACTGCCAACGGGAAT AGCCTGTCGTGACACCATTT ACAAGCAGCTGATCACACGA TGCTTTCATCTTTCTCAGGCAA ATATATTTCGCGGACAAGCGG
CaARF14 qRT
ATTGGTTGCTGGCAATGCTT
CaARF15 qRT CaARF16 qRT CaARF17 qRT CaARF18 qRT CaARF19 qRT CaARF20 qRT CaARF21 qRT CaARF22 qRT CaACTIN
TTCACCGTGGAGGCTTCTTC CGCGGAGGGTATTCAGCTTT AACCCGTGTGCTCTATCACG TCAGGAGCTGATTGCCAAGG TCGGAACAAGAACTGGCTGG TCACATGCAAGGGGTAGCTG GGTTTCTTGCAGTCCTCCGA ATGTGGAAGGAAGGACAGCC GCCCCTTGTTTGTGACAATG GGTACCATGGCAACGATTTATC TCTG GGTACCATGAAGGCACCATCA AACGG GGTACCATGGCAAACCTTTCTG GAAA
CaARF1 qRT CaARF2 qRT CaARF3qRT CaARF4 qRT CaARF5 qRT CaARF6 qRT
CaARF2 qRT CaARF10 qRT CaARF12 qRT
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Reverse primer(5'to3') GCCTTTTGTTCTTCCCTGCG GGGTATAGGGGAAGGGTGGT CACCACGCGGCATAGTATCT CGCCCCCAAAAAGGTAGACT CAACCTCCTTCTCCACGTCC GAGTTGCACGACGAATTCCC CCTCCCCGTGCAGTTATTGT CCGATTGGGTTTCCTGTCCA GCTGCAACCCCTTCAAAAGAA TGGTTTGGTATCAAGCTCAGTC AGCTGAGCTGGATGCAACAT AACACCGAGAAGAAGGTGCC CTGCTGCAGCTAGAAGACCA ATGAATCCCAAGACGAGAAGAA G GGTGGCCATCGAGAGGAAAA ACGTGACGCCTTCACAAAGA GTTGCGAGACCAGTGATCCA AGCCCGTTTCTAGGTCTTGC TAGCATCATAGCCTTCTGCAACA GCCAAGGATGATCGCCCATA CCAACAAGCCTTCTAGCCCA CAGTCTGGGGTCGAATTGCT ACACGAAGCTCATTGTAGAA GTCGACCAGAGCAATATCAAGA AGCA GTCGACTTCAGTTCCACTGCTAG CTT GTCGACTGCTTTCCCCTCATTGCT AA
