RP-HPLC Method Development and Validation for the Determination ...
Recommend Documents
Sep 24, 2016 - PMCID: PMC3480760. Development and Validation of RPHPLC Method for Simultaneous. Determination of Granisetron and Dexamethasone.
reproducibility. (GOLFINOPOULOS et al., 2001). However, the fibers are fragile and expensive, and some pesticides, such as DDT and endrin, suffer thermal.
Jul 11, 2013 - for the determination of nimodipine in sustained release tablets was developed. The method involved using a SinoChoom ODS-BP.
Recombinant human insulin was provided by Eli Lilly &. Co (USA). Water was Milli-Q grade and all other chemicals and solvents used were of analytical grade.
Oct 9, 2013 - apply a simple LC-MS/MS assay for quantification of testosterone level ... LC-MS/MS analysis was performed on Waters Alliance HPLC.
2Department of Pharmaceutical Analysis, K.V.S.R Siddhartha College of
Pharmaceutical Sciences, ... RP-HPLC method were developed and validated for
.
Wanda E. Whitfield, Elaine W. Gunter, Barbara B. Miller, Donald G. Patterson, Jr.,. Larry L. .... 5-Methyl- cotinine was a generous gift from Peyton Jacob, Univer-.
KAMINI KALRA, MANJEET AGGARWAL and R. K. KHANDAL*. Shriram Institute for ... Figure 1. Chemical structures of voglibose as per different publications.
Manual injections were made using a. Rheodyne .... Budavari S, O'Neil M, Ann Smith, Heckelman P and Obenchain J, Anastrozole, The Merck. Index An Encyclopedia of Chemicals, Drugs, and Biologicals, 12th Edition, Merck Research.
Sep 3, 2018 - Determination of Antidiabetic Drug Alogliptin Benzoate in. Bulk and Tablets ..... Industry, FDA, Silver Spring, MD, USA, 2015. [18] N. Batrawi, H.
Mar 15, 2012 - The separation was carried out on Merck HPTLC aluminum plates of silica ..... [5] M. J. O'Neil, The Merck Index: An Encyclopedia of Chemicals,.
Mar 15, 2012 - The separation was carried out on Merck HPTLC aluminum plates of silica ..... [5] M. J. O'Neil, The Merck Index: An Encyclopedia of Chemicals,.
RP-HPLC Method Development and Validation for the Determination ...
Mar 22, 2017 - validation of Alfuzosin HCl and Dutasteride in pharmaceutical dosage form by RP. HPLC. Int J Novel Tends PharmaSci 2015; 5: 94 101.
Indian Journal of Novel Drug Delivery 9(1), Jan-Mar, 2017, 38-43
Indian Journal of Novel Drug Delivery
IJNDD An Official Publication of Karnataka Education and Scientific Society
Research Article
RP-HPLC Method Development and Validation for the Determination of Alfuzosin HCl in Bulk and Pharmaceutical Dosage Form UMAY CHEN, SHADIA AFRIN, ANTARA GHOSH, SUJAN BANIK* Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh.
ARTICLE DETAILS
ABSTRACT
Article history: Received on 15 February 2017 Modified on 22 March 2017 Accepted on 25 March 2017
The present study was undertaken to develop a simple, rapid, accurate and sensitive reverse phase HPLC method for determination of Alfuzosin HCl in bulk powder and pharmaceutical dosage form. The chromatographic separation was accomplished on Hypersill ODS (250×4.6mm, 5μm) column using a mobile phase methanol: water is 90:10 (v/v) at a column temperature of 25°C. The eluents were monitored at 244 nm and total run time was 5 min with a flow rate of 1 ml/min. The drug was well resolved on the stationary phase and the retention time was found as 1.3±0.89 min with injection volume of 20µl.The method was found to be linear at concentration ranges of 10-50μg/ml with correlation coefficient of 0.999. The method was validated for linearity, precision, robustness and accuracy as per ICH guidelines. The results of all the validation parameters were well within their acceptance values (%RSD
