Supplemental figure S4: Annotated sequence ...

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Supplemental figure S4: Annotated sequence ...

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Supplemental figure S4: Annotated sequence alignment of wild type and G444C BLOC1S1 expression constructs. Annotation key: BLOC1S1 mRNA sequence ...

Supplemental figure S4: Annotated sequence alignment of wild type and G444C BLOC1S1 expression constructs. Annotation key: BLOC1S1 mRNA sequence, Translation start site in bold, numbering from start site Polyadenylation signal Guanine or Cytosine 444 qPCR tag Restriction sites for cloning WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

TATCGATCACGAGACTAGCCTCGACGTATACCCACGTGGTTTAAACACAC TATCGATCACGAGACTAGCCTCGACGTATACCCACGTGGTTTAAACACAC **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

AGCGGTCACGTGACATGGCCCCGGGGAGCCGAGGTGAGCGTTCCAGCTTC 36 AGCGGTCACGTGACATGGCCCCGGGGAGCCGAGGTGAGCGTTCCAGCTTC 36 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

CGGAGCCGGAGGGGGCCCGGCGTACCCAGCCCCCAGCCCGACGTGACCAT 86 CGGAGCCGGAGGGGGCCCGGCGTACCCAGCCCCCAGCCCGACGTGACCAT 86 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

GCTGTCCCGCCTCCTAAAAGAACACCAGGCCAAGCAGAATGAACGCAAGG 136 GCTGTCCCGCCTCCTAAAAGAACACCAGGCCAAGCAGAATGAACGCAAGG 136 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

AGCTGCAGGAAAAGAGGAGGCGAGAGGCTATCACTGCAGCGACCTGCCTG 186 AGCTGCAGGAAAAGAGGAGGCGAGAGGCTATCACTGCAGCGACCTGCCTG 186 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

ACAGAAGCTTTGGTGGATCACCTCAATGTGGGTGTGGCCCAGGCCTACAT 236 ACAGAAGCTTTGGTGGATCACCTCAATGTGGGTGTGGCCCAGGCCTACAT 236 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

GAACCAGAGAAAGCTGGACCATGAGGTGAAGACCCTACAGGTCCAGGCTG 286 GAACCAGAGAAAGCTGGACCATGAGGTGAAGACCCTACAGGTCCAGGCTG 286 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

CCCAATTTGCCAAGCAGACAGGCCAGTGGATCGGAATGGTGGAGAACTTC 336 CCCAATTTGCCAAGCAGACAGGCCAGTGGATCGGAATGGTGGAGAACTTC 336 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

AACCAGGCACTCAAGGAAATTGGGGATGTGGAGAACTGGGCTCGGAGCAT 386 AACCAGGCACTCAAGGAAATTGGGGATGTGGAGAACTGGGCTCGGAGCAT 386 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

CGAGCTGGACATGCGCACCATTGCCACTGCACTGGAATATGTCTACAAAG 436 CGAGCTGGACATGCGCACCATTGCCACTGCACTGGAATATGTCTACAAAG 436 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

GGCAGCTGCAGTCTGCCCCTTCCTAGCCCCTGTTCCCTCCCCCAACCCTA 486 GGCAGCTCCAGTCTGCCCCTTCCTAGCCCCTGTTCCCTCCCCCAACCCTA 486 ******* ******************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

TCCCTCCTACCTCACCCGCAGGGGGAAGGAGGGAGGCTGACAAGCCTTGA 536 TCCCTCCTACCTCACCCGCAGGGGGAAGGAGGGAGGCTGACAAGCCTTGA 536 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

ATAAAACACAAGCCTCCGTTTCTCGAGCCACGTATCGCCTGACTCTGTGG 586 ATAAAACACAAGCCTCCGTTTCTCGAGCCACGTATCGCCTGACTCTGTGG 586 **************************************************

WT_BLOC1S1_pRRLsin G444C_BLOC1S1_pRRLsin

TGTGTTTCAGACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGA 636 TGTGTTTCAGACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGA 636 **************************************************